배양된 치주인대세포와 치은섬유아세포에서 상이하게 발현된 유전자들의 검토 양상

윤혜정,최미혜,여신일,박진우,최병주,김문규,김정철,서조영,Yoon, Hye-Jeong,Choi, Mi-Hye,Yeo, Shin-II,Park, Jin-Woo,Choi, Byung-Ju,Kim, Moon-Kyu,Kim, Jung-Chul,Suh, Jo-Young 대한치주과학회 2006 Journal of Periodontal & Implant Science Vol.36 No.3

Periodontal ligament(PDL) cells and human gingival fibroblasts(HGFs) play important roles in development, regeneration, normal function, and pathologic alteration. PDL cells and HGFs have the similarity related with general characteristics of fibroblast such as spindle shaped morphology, the presence of vimentin intermediate filament and the synthesis of interstitial collagens and fibronectin. There were many studies about the differences between PDL cells and HGFs, but they were not about whole gene level. In this study, we tried to explain the differences of gene expression profiles between PDL cells and HGFs, and the differences among three individuals by screening gene expression patterns of PDL cells and HGFs, using cDNA microarray. Although there were some variants among three experiments, a set of genes were consistentely and differentially expressed in one cell type. Among 3,063 genes, 49 genes were more highly expressed in PDL cells and 12 genes were more highly expressed in HGFs. The genes related with cell structure and motility were expressed more highly in PDL cells. These are cofilin 1, proteoglycan 1 secretory granule, collagen type I(${\alpha}$ 1), adducin gamma subunit, collagen type III(${\alpha}$ 1), fibronectin, lumican(keratan sulfate proteoglycan), and ${\alpha}$ -smooth muscle actin. Tissue inhibitor of metalloproteinase known as the enzyme controlling extracellular matrix with matrix metalloproteinase is more highly expressed in PDL cells, osteoprotegerin known as osteoclastogenesis inhibitory factor is more highly expressed in HGFs. We performed northern blot to verify cDNA microarray results on selected genes such as tissue inhibitor of metalloproteinase, fibronectin, osteoprogeterin. The result of northern blot analysis showed that each cell expressed the genes in similar pattern with cDNA microarray result. This result indicates that cDNA microarray is a reliable method in screening of gene expression profiles.

장애인일자리사업과 삶의 질에 대한 동향 분석 : 2011년~2021년 논문을 중심으로

윤혜정(Yoon Hye Jeong),황예림(Hwang Ye Rim),윤수빈(Youn Su Bin) 한국장애인재단 2021 장애의 재해석 Vol.2021 No.-

본 연구는 장애인일자리사업과 삶의 질 관련 연구 동향을 분석하여 장애인일자리사업과 삶의 질 관련 연구에 대한 향후 연구 방향과 주제를 모색하고 활성화시키며, 근로 장애인을 위한 방안 마련과 제언적 패러다임을 제시하였다. 이는 현재 장애인일자리사업이 갖는 현실적인 문제와 요구에 대응할 수 있는 연구 방향에 길잡이가 되는 기초자료를 제공했다는 점에 의의를 두고 있다. 이를 위해 2011년부터 2021년까지 국내에서 발표된 학술논문, 학위논문(박사), 학위논문(석사) 34편을 대상으로 연구시기, 연구주제, 연구방법, 연구제언 동향 분석을 실시하였다. 그 결과가 다음과 같다. 첫째, 장애인일자리사업과 삶의 질 관련 연구는 2011년~2017년 사이 급증하였고, 그 이후 다소 주춤한 동향을 보이고 있다. 둘째, 장애인일자리사업과 삶의 질 관련 연구의 주제는 장애인 일자리사업과 영향요인의 주제에 편중되어 이루어져 온 것으로 나타났다. 셋째, 장애인일자리사업과 삶의 질 관련 연구의 대상은 장애인고용패널조사 등의 문헌대상이며 연구대상이 가장 다양하게 나타난 시기는 2017년~2019년이다. 넷째, 장애인일자리사업과 삶의 질 관련 연구의 자료수집방법은 양적연구가 32편(94%)을 차지하였으며, 양적 연구 중 실험법을 통한 연구가 대부분을 차지하였다. 마지막으로, ‘직무개발 및 배치를 통한 고용의 질 향상’, ‘물리적, 사회적인 근무환경 개선’ 등은 장애인일자리사업과 삶의 질 관련 연구에서 10년동안 꾸준하게 제시되는 제언이며 최근 2020년대에 들어서, 초기에는 제시되지 않은 새로운 제언들의 패러다임이 나타나고 있다. 본 연구는 양적인 분석과 적은 연구대상으로 심층적 분석에 어려움을 가짐을 밝히며 장애인일자리사업과 삶의 질에 대한 관심을 제고한다. 또한, 이에 관련된 현실적 문제를 개선하고 삶의 질 개선에 도움을 줄 수 있으며 현대적인 패러다임을 수반할 수 있는 연구가 필요하다는 것을 제안한다. This study analyzed research trends on job programs and quality of life for the disable d, sought and activated future research directions and topics on job programs and quality of life for the disabled, and suggested a new paradigm for the disabled. This is significant in that it provides basic data that serves as a guide to the direction of research that can respond to the practical problems and demands of the current job program for the disabled. To this end, from 2011 to 2021, 34 academic papers, dissertation (Doctor’s/Master"s thesis) published in Korea were analyzed for research period, research topic, research method, and research suggestion trend. The result is as follows. First, research on job programs and quality of life for the disabled increased rapidly between 2011 and 2017, and has been somewhat stagnant since then. Second, it was found that the topics of research related to job programs and quality of life for the disabled have been concentrated on the topics of job programs for the disabled and influencing factors. Third, the subjects of research on job programs and quality of life for the disabled are personal subjects such as the Employment Panel Survey for the Disabled, and the most diverse subjects were from 2017 to 2019. Fourth, 32 quantitative studies(94%) accounted for the data collection method of research related to job programs and quality of life for the disabled, and most of the quantitative studies were conducted through experimental methods. Finally, “improving the quality of employment through job development and placement” and "improving the physical and social working environment" are proposals that have been steadily presented for 10 years in research on job programs and quality of life for the disabled, and in the 2020s, a paradigm of new proposals has emerged. This study reveals that it is difficult to analyze in-depth with quantitative and small research subjects, and enhances interest in job programs and quality of life for the disabled. In addition, it is suggested that research that can improve the practical problems related to this reserch, help improve the quality of life, and involve a modern paradigm is needed.

사무직 직장인에게 스포츠 마사지가 체열과 피로도에 미치는 영향

윤혜정(Hye-Jeong Yoon),박동삼(Dong-Sam Park) 한국발육발달학회 2023 한국발육발달학회지 Vol.31 No.1

옥외 광고물의 외래어 사용실태 연구 - 대구지역을 중심으로 -

윤혜정 ( Hye Jeong Yoon ) 한국어의미학회 2000 한국어 의미학 Vol.7 No.-

This research purposes to closely analyze languages use for outdoor advertisements from the social-linguistic point of view and to find out the tendencies and the problems of them. Social-linguistic study is to contemplate group canon derived from calculated and averaged proverbs. Therefore, this study was based on field studies coupled with quantifical methods and was examined according to the morphologic and lexical theory. The study focuses on analyzing the actual state on borrowed words and characteristic forms of words used on the advertisements by the class, origin, structure, and character of them. In the section of the class, analysis was made for the distribution of native and foreign languages, chinese character, and peculiar letters. The origin part includes studies on the origin of foreign languages. As for the character part, forms of expression were examined by such languages as Korea, Roman, Chinese, et cereta. The structure section contains the examination of the structure on the words, phrases and sentences.

사회연결망분석을 활용한 거대사 교육 콘텐츠 개발 방향 제안

윤혜정(Hye-Jeong Yun),서희창(Hee-Chang Seo),박은수(Eun-Soo Park),이윤선(Yoon-Sun Lee),김재준(Jae-Jun Kim),이희수(Hee-Soo Lee),임성빈(Seong-Bin Lim),이태식(Tai-Sik Lee) 한국콘텐츠학회 2015 한국콘텐츠학회논문지 Vol.15 No.6

본 연구는 기존의 서양 중심 세계사를 바탕으로 한 암기식 교육의 문제를 개선 하고자 거대사 관점의 새로운 역사 교육 콘텐츠 개발 방향을 제시하는 것이 목적이다. 연구의 범위는 인류문화가 시작된 농업혁명 부터 산업혁명까지의 역사적 사건으로 설정하였다. 전문가들을 대상으로 한 델파이 기법을 통해 총 56개의 주요 역사적 콘텐츠를 도출하였고, 이를 사회연결망 분석의 데이터로 활용하였다. 사회연결망 분석을 통한 개별 콘텐츠의 영향력 분석 결과, 총 56개의 역사적 핵심사건 가운데 가장 영향력이 큰 사건의 키워드는 문명의 발생으로 나타났다. 또한 역사적 사건 키워드 네트워크를 구축하여 시각적으로 응집 분석한 결과 7개의 그룹으로 키워드가 분류되는 것으로 나타났다. 이러한 결과는 현행 교과과정의 시대별 분류기준과는 다른 새로운 분류기준으로 본 연구에서 제안한 역사 교육 콘텐츠 개발 체계는 거대사 관점에서 역사교육콘텐츠 네트워크를 구성한 새로운 역사 해석 방법이 될 것으로 기대한다. This study aims to provide suggestions for the development of educational contents on historical events that can solve the existing curriculum’s problems, such as the disproportionate weight given to Western historical events. The study focuses on content ranging from the start of the Agricultural Revolution (7000 BC.) to the start of the Industrial Revolution (AD. 1760). The results are as follows. We used the Delphi technique for deriving global historical events. Among them, 56 historical events were selected as the data for Social Network Analysis (SNA). The results of SNA showed that topics related to Civilization has a high priority. In addition, the results of a coagulation analysis showed the events can be divided into seven groups. The classification criteria is different from the criteria used for the current period. We expect that the suggested framework developed for historical contents will constitute a new approach to historical interpretation through network visualization and linkage analysis.

뇌성마비 환자의 전치부 수복

윤혜정(Hae Jeong Yoon),정복영(Bok-yeong Jung),김승혜(Seung-Hye Kim),송제선(Je Seon Song),이제호(Jae-ho Lee) Asia association of Disability and Oral health 2010 대한장애인치과학회지 Vol.6 No.1

Cerebral palsy is an umbrella term encompassing a group of non-progressive non-contagious motor conditions that cause physical disability in human development. Motor disorder of cerebral palsy is often accompanied by disturbances of sensation, perception, cognition, communication, behavior and seizure disorder. Disharmony of motor function leads to frequent falling down. Moreover patients have high prevalence of class II malocclusion. Compared to normal patients, the patients with cerebral palsy tends to have high prevalence of recurrent trauma and bruxism which make restoration of the anterior tooth more difficult. This case report is consisted of three cases of cerebral palsy patients who have challenging problems with restoration of anterior teeth.

한국 성인의 주관적 구취 자각증상에 대한 연구

윤미숙 ( Mi Sook Yoon ),윤혜정 ( Hye Jeong Youn ) 한국치위생과학회 2008 치위생과학회지 Vol.8 No.3

식품 중 수단색소의 분석법에 관한 연구

김희연(Hee-Yun Kim),윤혜정(Hae-Jung Yoon),최장덕(Jang-Duk Choi),최우정(Woo-Jeong Choi),박선영(Sun-Yong Park),이경주(Kyoung-Joo Lee),김지혜(Ji-Hye Kim) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.3

본 연구는 식품 중 식품에 사용이 불가한 수단 1호, 2호, 3호 및 4호 등 4종의 유용성색소에 대한 안전성 확보 및 효율적 품질관리를 위해 박층크로마토그래피(TLC)와 고속액체 크로마토그래피(HPLC-PDA)를 이용한 정성, 정량분석법을 확립하고자 수행하였다. C18-silica를 사용하여 methanol : water(95 : 5)으로 전개하였을 때 수단색소들의 R_f 값은 0.27~0.59로 산출되었다. HPLC-PDA를 이용하여 water와 acetonitrile 용매를 사용하여 분석한 결과 20분내에 모두 분리되었으며 검량선의 농도 범위는 0.1~100.0 ㎍/mL으로 나타났다. 본 연구에서 고춧가루, 김치 및 깍두기 등 식품을 대상으로 적용한 결과, 회수율은 고춧가루에서는 85.84~96.95%, 김치에서는 86.24~104.30%, 깍두기에서는 83.02~99.93%의 양호한 결과를 얻었으며, 검출한계는 수단 1호 및 2호는 0.01 ㎍/mL, 수단3호 및 4호는 0.02 ㎍/mL으로 나타났다. A simple, efficient and accurate method was developed for the simultaneous determination of non-permitted oil soluble colorants (sudan Ⅰ, Ⅱ, Ⅲ and Ⅳ) in foods. The identification has been carried out for sudan colorants by TLC as well as HPLC with photodiode array (PDA) detection. Separation of sudan colorants was achieved within 20 min by a gradient elution with water and acetonitrile as eluents. Sudan colorants showed good linear relationships in the range of 0.1~100 ㎍/mL. The correlation coefficients of the calibration curve for sudan colorants exceeded 0.999. The detection limits (signal-to-noise ratio 3 : 1) for sudan Ⅰ, Ⅱ, Ⅲ and Ⅳ were 0.01, 0.01, 0.02 and 0.02 ㎍/mL, respectively. This method has been successfully applied to the analysis of red pepper powder, Kimchi and Kakdugi, and the average recoveries for real samples ranged from 83.02% to 104.3%.

식품 중 인공감미료의 분석법에 관한 연구

김희연(Hee-Yun Kim),윤혜정(Hae-Jung Yoon),홍기형(Ki-Hyung Hong),이창희(Chang-Hee Lee),박성관(Sung-Kwan Park),최장덕(Jang-Duck Choi),최우정(Woo-Jeong Choi),박선영(Sun-Young Park),김지혜(Ji-Hye Kim),이철원(Chul-Won Lee) 한국식품과학회 2004 한국식품과학회지 Vol.36 No.1

일부 치위생과 학생의 식이섭취실태와 치아우식증과의 상관관계 연구

윤미숙 ( Mi Sook Yoon ),정은주 ( Eun Ju Jung ),윤혜정 ( Hye Jeong Youn ) 한국치위생과학회 2012 치위생과학회지 Vol.12 No.2

The purpose of this study was to utilize it as useful basic data for improving oral health and for developing educational guidelines, by surveying the intake frequency in cariogenic food and the dietary intake status in detergent food through dietary life diary of some female undergraduates, and by researching into relationship between dietary intake factor and dental caries. It allowed dietary life diary for 5 days to be recorded targeting female undergraduates for the Dept. of Dental Hygiene, selected dietary life diary of 66 people without omission of record, and used it in this study. The following conclusions were obtained. 1. As a result of surveying the food intake status, it was indicated to be 12.82 for cariogenic food intake frequency, 256.36 for caries incidence available time and 8.32 for detergent food intake frequency. 2. As a result of surveying in order to examine relationship between cariogenic food intake frequency, detergent food intake frequency & cariogenic light-meal intake frequency and dental caries by dividing group with caries and group with non-caries, the group with caries was indicated to be a little higher than the group with non-caries. However, statistically significant difference wasn`t indicated. 3. In the correlation between dietary intake status and dental caries, it was indicated that the more rise in cariogenic food intake frequency leads to the more rise in detergent food intake frequency and even in total light-meal intake frequency, but wasn`t shown correlation with dental caries. In light of the above results, many limitations were indicated in finding and analyzing the dental caries incidence level and cause just with a method through surveying dietary intake. However, given the more in cariogenic food intake frequency and detergent food intake frequency, the possibility of dental caries incidence could be predicted.