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Fe(lll) 환원능을 가진 Cellulomonas(Cellu-2a) 균주에 의한 Cr(VI)의 직 · 간접적 환원
어나미까커날 ( Anamika Khanal ),이지훈 ( Ji-hoon Lee ) 한국환경농학회 2020 한국환경농학회 학술대회집 Vol.2020 No.-
Heavy metals contamination and health risk assessment issues have been raised as one of the global concerns. Even though there are various strategies ongoing for the remediation of such pollution, the use of direct and indirect microbial reduction of heavy metals offers a potential mechanism for immobilizing, transforming, or reducing some soluble form of heavy metals to insoluble forms. Besides that these methods are cost-effective and environmentally friendly too. In this study, fermentative Fe(III)-reducing bacterial strains (Cellu-2a, Cellu-5a, and Cellu-5b) from the groundwater in an anaerobic condition has been isolated and characterized by an emphasis on iron reduction and iron biomineralization. The isolated colonies were found to have 99 % similarities with Cellulomonas species. Biochemical characterization of these colonies revealed to utilize L-arabinose, D-xylose, D-glucose, D-fructose, D-mannose, D-mannitol, N-acetylglucosamine, Esculine ferric citrate, Salicin, D-saccharose, D-xylose, D-galactose, D-cellobiose, Amidon (starch), Glycogen, D-trehalose within 24 hr to 48 hr. Among these three strains, Cellu-2a was used for further analysis. The isolated strain Cellu-2a showed the reduction of ferric citrate (2 mM) and HFO (2 mM) as well as direct and indirect reduction of Cr(VI) (15 uM) in a time-dependent manner using sucrose as a carbon source.
실시간 중합효소연쇄반응을 이용하여 논 토양 내 질소순환 관련 기능 유전자의 평가
어나미까커날 ( Anamika Khanal ),이슬 ( Seul Lee ),여준구 ( Junkoo Yeo ),노그라도캐시린 ( Kathyleen Nogrado ),조아현 ( Ahyeon Cho ),송윤진 ( Yoonjin Song ),권미지 ( Miji Kwon ),이지훈 ( Ji-hoon Lee ) 한국환경농학회 2018 한국환경농학회 학술대회집 Vol.2018 No.-
The nitrogen cycle and the microbes responsible for mediating this process have an important role in natural ecosystems, terrestrial habitats and also have a major impact on climate change. In this study, we use molecular techniques such as conventional PCR, clone library construction, sequencing, phylogenetic analysis and real-time PCR to explore microbial communities in a rice paddy soil by detecting and quantifying some of the key functional genes that are involved in the process of nitrogen cycle i.e., nitrogen fixation (nifH), hydrazine synthase (hzsA), nitrous oxide reductase (nosZ), copper-containing (nirK), cytochrome cd1- containing nitrite reductase (nirS), nitrite oxidoreductase (nxrB), and ammonium monoxygenase (amoA). The sequence assessment from the clone library targeting these different genes i.e., nifH, hzsA, nosZ, nirK, nirS, nxrB, and amoA showed a high diversity and dominance of bacterial community. Besides that, Real-time PCR using SYBR green dye and some old and recently developed primers specific for each individual gene revealed the high abundance of nxrB gene and low abundance of hzsA gene. This finding will be very helpful in exploring and exploiting microorganism in terrestrial habitat.
살코스키 시약과 Indole-3-acetic Acid의 반응 중 트립토판의 간섭
이슬 ( Seul Lee ),어나미까커날 ( Anamika Khanal ),여준구 ( Junkoo Yeo ),노그라도캐시린 ( Kathyleen Nogrado ),조아현 ( Ahyeon Cho ),송윤진 ( Yoonjin Song ),권미지 ( Miji Kwon ),이지훈 ( Ji-hoon Lee ) 한국환경농학회 2018 한국환경농학회 학술대회집 Vol.2018 No.-
Indole-3-acetic acid (IAA) is the most common auxin hormone produced in the plant and is very important because it regulates various aspects of plant growth and development. Tryptophan is the main precursor for IAA while IAA can be synthesized by two pathways i.e., tryptophan-dependent pathway and tryptophan-independent pathway. There have been many studies using Salkowski regent to measure the IAA production by bacteria. In this study, we have found that there was the interference of tryptophan on the colorimetric estimations of indole-3-acetic acid (IAA) by using Salkowski reagent (35% HClO4 and 10 mM FeCl3), which developed a pink color complex of tris-(indole-3-acetate) iron(III) with IAA. Moreover, we detected the effect of different concentration of tryptophan (100, 200, and 300 μM) on different concentration of IAA (100, 200, and 300 μM). The absorbance on IAA for the tested concentrations was found to be increased with the increasing concentration of tryptophan. It seems that both the IAA and tryptophan show the maximum absorbance at the similar wavelengths. It was also observed that the difference between the predicted and measured concentrations of IAA became larger as the predicted IAA concentration decreased. From this study, it is expected that tryptophan would interfere with accurate measurements of IAA, and therefore care should be taken for the measurement of IAA generated by the precursor, tryptophan.