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K-562 백혈병 세포주에서 저근백피와 Gleevec을 처리에 의한 유전자 발현 비교 분석
차민호,안원근,전병훈,윤용갑,윤유식,Cha, Min-Ho,An, Won-Gun,Jeon, Byung-Hun,Yun, Yong-Gab,Yoon, Yoo-Sik 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3
In this study, we investigated gene expression patterns induced by Ailanthus altissima extract and compared it with Gleevec, a well-known anti-leukemia drug, in K562 chromic leukemia cells. Ailanthus altissima extract(100 ug/ml) and Gleevec(50 ug/ml) were treated to cells for 1h, 2h, 4h, and 16h and total RNA was extracted. Gene expressions were evaluated using cDMA microarray, in which 24,000 genes were spotted. Hierarchical clustering analysis showed that expression of genes included in two clusters were increased or decreased time dependently by both Ailanthus altissima extract and Gleevec. Genes included in another cluster were induced by Ailanthus altissima extract but not by Gleevec. In biological process analysis, expression of genes involved in apoptosis, growth arrest and DNA-damage were increased, but genes stimulating cell cycle were decreased. This study provides comprehensive comparison of the patterns of gene expression changes induced by Ailanthus altissima extract and Gleevec in K-562 leukemia cells.
참옻 추출물의 MCF-7 인체 유방암 세포에서 증식 억제효과
김민성,안원근,이장천,Kim, Min Sung,An, Won Gun,Lee, Jang Cheon 대한한의학방제학회 2016 大韓韓醫學方劑學會誌 Vol.24 No.4
Objectives : The purpose of this study was to investigate the anti-cancer effects of extract of Rhus verniciflua Stokes (RVS) in human breast cancer cell lines. Methods : In cultured human breast cancer MCF-7 cells, we investigated growth inhibitory effect of RVS. MCF-7 cells were cultured with various concentrations (0, 200, 300, and 400 ug/ml) of RVS at $37^{\circ}C$ for 24 h. We performed CCK-8 assay and flow cytometry for detection of Annexin V-PI staining. Results : As a result, RVS inhibits the cell growth and induction of apoptosis in dose dependent manner in MCF-7 breast cancer cells. Conclusion : RVS has anti-cancer activities and induced apoptosis in human breast cancer MCF-7 cells. Therefore we suggest that RVS can use as a novel class of anti-cancer drugs.
Anti-Inflammatory and Antioxidant Effect of Astaxanthin Derived from Microalgae
곽태원(Tae Won Kwak),차지영(Ji Young Cha),이철원(Chul Won Lee),김영민(Young Min Kim),유병홍(Byung Hong Yoo),김성구(Sung Gu Kim),김종명(Jong-Myoung Kim),박성하(Seongha Park),안원근(Won Gun An) 한국생명과학회 2011 생명과학회지 Vol.21 No.10
Astaxanthin (ATX)은 다양한 생명체에서 생성되는 카로티노이드 색소이다. 본 연구에서는 ATX가 RAW264.7 cell에서 LPS에 의한 inducible nitric oxide synthase (iNOS), nitric oxide (NO), 염증성 사이토카인, nuclear factor-kappa B (NF-κB)와 reactive oxygen species (ROS)의 생성을 억제 시키는 지 또한, superoxide radical 소거능이 있는 지를 조사하였다. iNOS와 NF-κB는 immunoblot analysis로, interleukin (IL)-6와 tumour necrosis factor-α (TNF-α)는 ELISA 법으로 분석하였다. NO 양은 nitrite의 양을 측정하였고, ROS는 2",7"-dichlorodihydro-fluorescin diacetate (DCFH-DA) 법으로 superoxide radical 소거능은 superoxide radical scavenging activity assay로 검증하였다. 100 μM의 ATX 농도에서 LPS로 유도된 NO, IL-6 및 TNF-α 같은 염증성 사이토카인의 생성 뿐만 아니라 iNOS 및 NF-κB의 발현도 억제되었다. 특히, IL-6 및 TNF-α 생성에 있어 ATX의 최대 억제율은 각각 65.2% 및 21.2% 이었으며 LPS로 유도된 NF-κB의 전사활성을 억제하였다. 이러한 현상은 세포질에서 핵으로 NF-κB의 전위를 억제하는 것과 관련이 있다. 또한, 25-100 μM의 ATX 농도에서 세포 내 ROS 생성을 억제하였으며, 5 mg/ml 농도의 ATX는 동일농도의 α-tocopherol에 비해 superoxide radical 소거능이 1.33배 높았다. 이러한 결과들은 ATX가 대식세포에서 ROS 생성 및 NF-κB 활성을 저해하므로 iNOS의 발현, NO 및 염증성 사이토카인의 생성을 억제하며, 또한 우수한 superoxide radical 소거능을 보유한다는 것을 나타내었다. 결론적으로, ATX가 항염증제 및 항산화제로서 유용하게 사용될 수 있을 것으로 사료된다. Astaxanthin (ATX) is a red-orange carotenoid pigment that occurs naturally in a wide variety of living organisms. In this study we investigated the inhibitory effects of ATX on the induction of inducible nitric oxide synthase (iNOS), nitric oxide (NO), proinflammatory cytokines, nuclear factor-kappa B (NF-κB) and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. In addition, we tested the superoxide radical scavenging activity of ATX by scavenging assay. iNOS and NF-κB expressions were determined by immunoblot analysis. Interleukin (IL)-6 and tumour necrosis factor-α (TNF-α) were assayed by ELISA. NO production was monitored by measuring the amount of nitrite. ROS was examined by using the 2", 7"-Dichlorodihydrofluorescin diacetate (DCFH-DA) method. At a concentration of 100 μM, ATX inhibited the expression level of LPS-induced NF-κB, as well as the production of LPS-induced NO and proinflammatory cytokines (IL-6 and TNF-α), by suppressing iNOS expression. In particular, the maximal inhibition rate of IL-6 and TNF-α production by ATX (100 μM) was 65.2% and 21.2%, respectively. In addition, ATX inhibited the LPS-induced transcriptional activity of NF-κB, and this was associated with suppressing the translocations of NF-κB from the cytosol to the nucleus. Moreover, at various concentrations (25-100 μM), ATX inhibited the intracellular level of ROS. At a concentration of 5 mg/ml, the superoxide radical scavenging activity of ATX was 1.33 times higher than α-tocopherol of the same concentration. These results showed that ATX inhibited the expression of iNOS and the production of NO and proinflammatory cytokines resulting from ROS production and NF-κB activation in macrophages. Furthermore, ATX was found to be more effective in superoxide radical scavenging activities compared to α-tocopherol. These findings are expected to strengthen the position of ATX as anti-inflammatory medicine and antioxidant.