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단세포전기영동법(Single Cell Gel Electrophoresis Assay)을 이용한 농약 살포자의 DNA손상 평가
이연경(Yeon Kyeng Lee),이도영(Do Young Lee),이은일(Eunil Lee),이동배(Dong Bae Lee),류재천(Jae Chun Ryu),김해준(Hae-Joon Kim),설동근(Donggeun Sul) 한국환경성돌연변이발암원학회 2001 한국환경성돌연변이·발암원학회지 Vol.21 No.2
Single cell gel electrophoresis (SCGE) assay, also called comet assay, is a rapid and sensitive method to detect DNA damage in single cell level. To evaluate the DNA damage of lymphocytes of pesticides sprayers, SCGE assay was carried out for 50 pesticides sprayers and 58 control subjects. They were interviewed with structured questionnaire to get the information about the kinds and amount of pesticide. Insecticides and fungicides were predominant among pesticides. Major components of pesticides were organophosphorus, organosulfate, cartap, carbamates, and triazole. Sprayed pesticides were classified into two groups. Group I included organophosphorus, organoarsenic, organotin, tetrazine, triazole and gramoxone, which were known to cause DNA damages. Group II pesticide were carbamates, surfactants, organosulfates, etc., which were not found as DNA damaging agents in scientific documents. Olive tail moments of 100 lymphocytes were measured by<br/> KOMET 3.1 program for each person. The means of tail moments were compared between farmers exposed to pesticides and control subjects. Farmers showed higher tail moments than control subjects (2.07±1.40 vs 1.53±0.77, p<0.05). The means of tail moments also were compared among group I sprayers (n=36), group II sprayers (n=24) and, control subjects, and the means of tail moments were 3.45±3.20, 2.66±2.20 and 1.53±0.77 respectively. The difference between means of group I sprayers and controls was statistically significant (p<0.05). In conclusion, this study showed higher DNA damage in farmers exposed to pesticides than control subjects, and<br/> comet assay could be useful as a biological monitoring method of genotoxic pesticides for farmers.
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