Evaluation of Matrix Effect in Body Fluid Chemistry on Roche Cobas 8000 c702 System

서종도,이지교,김승환,남영원,이준희,이경훈,송정한,송상훈 대한진단검사의학회 2021 Laboratory Medicine Online Vol.11 No.4

Background: Analysis of body fluids aids in diagnosis and monitoring disease. However, only a few testing platforms and reagents have been validated for a range of body fluids or analytes. In this study, we evaluated a testing system, which has been approved for blood samples, in analyzing body fluid specimens upon matrix mixing. Methods: Serum and body fluid samples, including cerebrospinal fluid (CSF), ascites, pleural fluid, amniotic fluid, and synovial fluid, were mixed, then the matrix effect and linearity for major analytes, namely amylase, chloride, glucose, LDH, and protein were evaluated (N = 30 serum-body fluid pairs) on the Cobas 8000 c702. The obtained data was compared with that of open reagents evaluated on the Architect c16000. Results: For all analyte-body fluid pairs, the mean percent recovery ranged from 98.4% to 101.7%, and this was within the acceptable range for matrix effect. In the linearity test, maximum non-linearity for each analyte-body fluid pair ranged from -5.0% to +4.2%. In interference test, proteins showed positive hemolytic, icteric, and lipemic interference in CSF and hemolytic interference in amniotic fluid. There was no significant interference in the other analyte-body fluid pairs. Results were highly correlated between the Cobas 8000 c702 and the Architect c16000 system. Conclusions: Our findings revealed that the matrix effect of major analytes in body fluid specimens can be excluded and they also validated the linearity of the analytes in the body fluid specimens. Therefore, reagents specified for blood samples can be readily adopted for the analysis of body fluids.

Evaluation of Test Standardization for 25-OH Vitamin D Immunoassays

서종도,이준희,조성은,송정한,이용화,윤여민 대한진단검사의학회 2024 Laboratory Medicine Online Vol.14 No.1

Background: Accurately measuring 25-OH vitamin D (25-OH-D) is important for monitoring vitamin D status. Despite the efforts of all involved parties, achieving standardization remains challenging. The present study assessed the standardization status of commercial immunoassays for measuring 25-OH-D. Methods: The diagnostic accuracy of commercial immunoassays Roche Elecsys Vitamin D Total, Siemens Atellica IM Vitamin D Total, Abbott Alinity 25-OH Vitamin D, and Beckman Coulter Access 25-OH Vitamin D Total was evaluated by comparing the quantitative values and qualitative decisions for 150 residual serums with various concentrations obtained from each test to the results determined using mass spectrometry (MS). Then, correlation and concordance were estimated. Results: When the measured 25-OH-D values from each assay were compared to those determined using MS ranging from 5.86 to 67.75 ng/mL, no evaluated immunoassay was equivalent to MS by showing slope and intercept not containing 1 and 0 in their 95% confidence interval, respectively. Regression estimates at the medical decision limits of 10, 20, and 30 ng/mL showed that only 30 ng/mL of the Beckman Coulter test was within the acceptable bias limit of 5.0% recognized by the VDSCP. The concordance of decisions with MS ranged from 90.0 to 94.0% in immunoassays. Conclusions: Despite achieving the certification by the VDSCP, most immunoassays differed significantly regarding acceptance criteria in measuring 25-OH-D, which may affect clinical decision-making. Therefore, attention should be paid to interpreting the results, and further efforts for test standardization are required.

사망유형 분석을 위한 자살위험요인 탐색 : 사례-대조 심리부검 연구를 중심으로

서종한,김나애,김경일 대한범죄학회 2018 한국범죄학 Vol.12 No.3

The purpose of this research is to identify risk factors that can classify the cause of different types of deaths, including suicide, through case-controlled psychological autopsy study. The criteria for judging suicide patterns were largely divided into four important areas, referring to prior study results from national and international studies (Sneedman, 1976; Litman, 1968; Seo Jong Han et al., 2012; Seo Jong Han et al., 2018). Four areas were divided into: problems in life or adaption, suicide/death attempts in the past, physical and mental health characteristics, and information related to suicidal deaths such as family, friends or other acquaintances. We looked the differences between 122 cases of suicide and 40 cases in the control group. The suicide cases group was divided by generations to see if there were any noticeable differences in the causes of suicide between generations. As a result, we found that job, education and economic conditions, social network, past self-suicidal attempts, physical and mental health, and surrounding factors were highly related to suicides. Each factor was compared to a national situation based on the results of an overseas study, and additional specific information and implication related to each factor were discussed. 본 연구는 사례-대조 심리부검 연구를 통해 자살을 포함한 사망 유형의 구분 및 자살의 원인 분석을 용이하게 할 위험요인을 찾아보았다. 국내외의 심리부검 선행연구와 문헌을 참고하여 자살 유형 판단 기준을 크게 네 가지로 나누어 (Shneidman, 1969; Litman, 1968; 서종한 등, 2012; 서종한 등, 2018) 그 안에서 자살사 사례군 122건과 대조군 40건 간의 차이를 알아보았다. 또한, 자살 사례군을 세대별로 나누어 각 세대 간에 두드러진 차이가 나타나는 자살 요인의 있는지도 알아보았다. 그 결과, 자살과 관련성이 높은 요인들로 1) 직업, 교육, 경제적 상태, 2) 사회적 관계망, 3) 과거 자해 및 자살시 도, 4) 신체 및 정신건강 상태, 5) 주변인 등의 요인들이 관련 있는 것으로 확인되었다. 각 요인은 해외 연구결과를 토대로 국내의 상황과 비교되었으며, 구체적 정보 및 시사점이 추가로 논의되었다

A Case of Fungemia with Co-isolation of Candida parapsilosis and Trichosporon asahii Confirmed by MALDI-TOF MS and D2 rRNA Sequencing

서종도,김남희,홍윤지,김택수,황상미,박정수,성문우,박경운,송정한,박성섭,김의종 대한진단검사의학회 2019 Laboratory Medicine Online Vol.9 No.2

Fungi are a major cause of human infections with diverse clinical manifestations. The incidence of fungal infections has increased over time, particularly in patients who have risk factors such as neutropenia, immune suppression, an intravascular catheter, parenteral nutrition, a prosthetic device, and prior broad spectrum antibiotic therapy. Here, we present an unusual case of co-infection by 2 distinct fungi, Candida parapsilosis and Trichosporon asahii, isolated from a patient who did not have any known risk factors initially, except active pulmonary tuberculosis. Despite the negative conversion of sputum acid-fast bacilli (AFB) culture test after treatment, clinical symptoms were refractory to therapy. The patient developed symptoms suggesting septic shock, and 2 distinct colonies were isolated from a blood specimen, which were identified as C. parapsilosis and T. asahii by MALDI-TOF and rRNA sequencing. Fever and hypotension were relieved after anti-fungal agent injection, and pulmonary lesions identified by imaging also improved.

안전 코칭 프로그램이 안전행동과 안전 분위기에 미치는 효과 -고속도로 안전순찰원을 중심으로-

서종도,석봉준,문광수 한국안전학회 2024 한국안전학회지 Vol.39 No.2

- This study investigated the impact of a safety coaching program on the safety behavior and safety climate among expressway safety patrols. Four to seven patrols from each of the three branches participated in this study. The safety coaching program was developed based on the GROW model, with main contents including recognizing individual differences, exploring safety values, communicating near-misses, providing effective and efficient feedback, employing non-violent communication, and fostering commitment toward safety behaviors. Additionally, each session included self-monitoring and peer review of each item based on a critical behavior checklist developed for this study, with challenging goals set based on the monitoring and review. The safety coaching program comprised six sessions in three branches, while three other branches were assigned as a control group. A non-equivalent control group experimental design was applied. Dependent variables included observed and perceived safety behavior, safety climate, psychological safety, and feedback. The results indicated that the safety coaching program effectively increased patrols’ safety behavior and safety climate. Furthermore, psychological safety and feedback improved. These findings suggest that the developed safety coaching program could serve as an alternative method to enhance safety management for expressway safety patrols. Finally, the implications, limitations, and directions for future research are discussed.

한국사회에서의 사이코패스 개념, 믿음, 태도에 관한 연구 : 사이코패스성격장애종합평가(CAPP)를 중심으로

서종한 ( Jonghan Sea ),신강현 ( Kanghyun Shin ),김경일 ( Kyungil Kim ) 대한범죄학회 2018 한국범죄학 Vol.12 No.2

본 연구는 국내 일반인들이 사이코패스에 대해 갖는 원형성을 살펴봄으로써 사이코패스성격장애종합(CAPP)평가의 내용타당도를 확인하는데 있다. 이를 위해 10차례의 번역과 역번역을 실시하여 최종 번역본을 완성하였다. 또한 남성과 여성 사이코패스에 대해 갖는 원형성에 어떤 차이점이 있는지 조사하고자 하였다. 더 나아가 일반 대중들이 사이코패스 갖는 인식, 태도, 가치 등 신념에 대해 탐색적 수준에서 살펴보았다. 이를 위해 1,021명의 일반 대중들을 대상으로 온라인과 오프라인을 이용하여 설문조사를 실시하여 결과를 얻었다. 분석 결과, 사이코패스증상이 이와 상관없는 증상과 유의미하게 높은 원형성을 보였고 2개의 외국 집단과 비교한 결과 유사한 패턴을 보였다. 또한 여성이 남성사이코패스보다 수다스런, 의심하는, 감정 안정성이 부족한, 가식적인, 불안정한 자기개념 등에서 차별성을 보였다. 일반인들이 사이코패스에 대해 갖는 태도는 사이코패스가 더 심각한 수준의 폭력 잠재성이 존재하는 부분에 대해서는 모두 공감하고 있었다. 그리고 옳고 그름을 이해할 수 없어 일종의 정신병자 내지는 사회악 혹은 악마로 규정하며 앞으로 변화될 여지가 없는 불변의 특성으로 받아들이고 있었다. 따라서 더 강력한 사법적 치료와 통제가 필요한 부분에 대해서는 공감하고 있었고 일부 이들의 친사회적 기술 등은 인정하고 있지만 직업이나 인생에서 성공하지 못할 것으로 보았다. 사이코패스 범죄에 대한 불필요한 두려움과 불안감에 일정 부분 기여하는 것으로 보인다. 이 관련하여 본 연구의 시사점과 한계, 향후 연구에 대해 구체적으로 논하였다. This study aimed to investigate the content validity of CAPP(Cooke et al., 2004) through prototypicality analysis (Kreis et al., 2012). We conduct ten-(back-)translation procedures of the original version of the CAPP until completed. Then we explored the differences and similarities between male and female psychopathy through prototypicality analysis. Moreover, various perception, attitude, and beliefs concerning psychopathy in lay people were comprehensively explored. Thus this study surveyed 1,020 lay people regarding various perception, attitudes, and beliefs they had concerning psychopathic personality (i.e., psychopathy). The questionnaire designed by Smith et al. (2014) was used, which consists of 5 domains(criminality, rehabilitation potential, ethological underpinnings, moral judgement, legal sanctions) and 28 items as a form of question toward lay people. Principally in concordance with previous results of earlier studies using lay people raters, they rated most of the 33 CAPP items and 6 CAPP domains as at least moderately prototypical, and compared to two abroad samples(e.g., Spain, North America), it displayed very similar pattern among 33 CAPP symptoms which were different from 9 foils. Also, female psychopath were significantly rated more prototypical in 4 symptoms such as Garrulous, Lacks emotional stability, Insincere, and Unstable self-concept. Lay people viewed psychopath as much severe violent in criminal potentiality, as not capable of determining right from wrong, and as generally ‘insane’ or ‘evil’. It means they recognized that psychopath can not change or rehabilitated. Accordingly, they endorsed that psychopathy should be incarcerated and even acknowledging some pro-social features in psychopathy, they not successful in life. These perceptions and belief may contribute to excessive fear of psychopathic crime and negative attitude in dealing with psychopathy. Finally, implication and limitations of the study are discussed and suggestions are added for later research direction.

Bel-Canto 창법에 대하여

서종일 서라벌문화예술제작소 1971 예술 서라벌 - 서라벌문화예술제작소 Vol.6 No.1

우울증과 방화사건 간의 연관성 사례연구

서종한,김병수,박조연 한국법과학회 2012 한국법과학회 학술대회 Vol.2012 No.1

Yoshimura 실린더 구조의 효율적인 수납 방법 및 실험적 검증

서종은 (Jong-Eun Suh),김태현(Tae-Hyun Kim),양진규(Jinkyu Yang),한재흥(Jae-Hung Han) 한국항공우주학회 2020 한국항공우주학회 학술발표회 논문집 Vol.2020 No.11

모바일 기기의 스크롤 조작 UI에 Vibrotactile Flow 적용

서종만(Jongman Seo),최승문(Seungmoon Choi) 한국HCI학회 2014 한국HCI학회 학술대회 Vol.2014 No.2