시뮬레이션을 이용한 응급실 위기관리 팀 훈련 프로그램의 개발 및 시범적용

백희진,정시경,박상현,박규남,박지은,김영민 대한응급의학회 2008 대한응급의학회지 Vol.19 No.6

Purpose: Emergency departments (EDs) are complex and dynamic working environments in which crises can easily develop. ED members must be trained to recognize and prevent impending crises or resolve active ones. Patient simulation (PS) is emerging as an effective tool in team training. We investigate the participants’ responses to a simulation-based ED Crisis Management Team Training (CMTT) program we developed and applied. Methods: We developed a pilot 2-hour simulation-based ED-CMTT program and applied to ED members who worked in three different types of EDs. The program was consisted of a lecture for principles of crisis resource management (CRM) and team dynamics, orientation to simulator and simulation environment, PS session with videoassisted debriefing and survey. After finishing the program, participants completed a survey and were solicited comments. Results: Fifty-two trainee participated in more than one of total seven pilot programs. The responses for the program are as follow: enjoyable and satisfied, 4.00±0.74; helpful for their teamwork, 3.98±0.73; understanding of CRM principles, 4.00±0.63; attainment of learning objectives, 3.80± 0.83; quality of faculty, 3.90±0.63; time allocation, 3.40± 0.70. The responses for PS session are as follow: realism of scenario, 3.98±0.63; realism of simulator, 3.10±1.05; realism of simulation environment, 3.44±0.98; quality of audio-visual system, 3.81±0.74. Overall comments were positive and commonest request was to have more experiences in variable scenarios. However, some problems like time shortage and lack of realism were also pointed. Conclusion: The participants’ reaction show that the simulation- based ED-CMTT program was well accepted by ED members. Creating more variable crisis scenarios and realistic simulation setting will be necessary for improving the program.

에토프로포스 살충제를 분해하는 토양 미생물 Sphingobium sp. EP60845 분리 및 특성

김수연,백희진,최수지,김지선,이창묵,권순우,한범수,김동험,윤상홍,심준수 한국국제농업개발학회 2017 韓國國際農業開發學會誌 Vol.29 No.3

An organophosphorus pesticide, ethoprophos, has been widely used in agriculture to control major insect pests. As ethoprophos is a well-known neurotoxin, its accumulation in soils and groundwater is concerning worldwide. In this study, we constructed an artificial ethoprophos-enrichment soil system, and its active concentration in soils was measured by gas chromatography on 15-days intervals during 90 days after ethoprophos treatment. Furthermore, the changes in bacterial community and microorganisms responsible for efficient bioremediation were investigated while ethoprophos was degraded in soils. From 15 to 60 days after the treatment, ethoprophos was actively degraded in soils and members of genera Collimonas and Sphingobium appeared dominantly in a natural microbial community especially in 60-days-after-treatment soil. We isolated a bacterium EP60845 from this soil sample, showing significant ethoprophos biodegradation activity in vitro. When we challenged EP60845 strain into ethoprophos-enrichment soils (250 mg/kg of soil), most ethoprophos was removed within 5-days. Phylogenetic 16S rRNA gene sequence analysis and biochemical properties by API 20GN kit demonstrated that the EP60845 strain was a novel Sphingobium sp., which could be used as an efficient ethoprophos- degrading agents for bioremediation purposes.

Chest compression quality, exercise intensity, and energy expenditure during cardiopulmonary resuscitation using compression-to-ventilation ratios of 15:1 or 30:2 or chest compression only: a randomized, crossover manikin study

곽세정,김영민,백희진,김세홍,임현우 대한응급의학회 2016 Clinical and Experimental Emergency Medicine Vol.3 No.3

Objective Our aim was to compare the compression quality, exercise intensity, and energy expenditure in 5-minute single-rescuer cardiopulmonary resuscitation (CPR) using 15:1 or 30:2 compression-to-ventilation (C:V) ratios or chest compression only (CCO). Methods This was a randomized, crossover manikin study. Medical students were randomized to perform either type of CPR and do the others with intervals of at least 1 day. We measured compression quality, ratings of perceived exertion (RPE) score, heart rate, maximal oxygen uptake, and energy expenditure during CPR. Results Forty-seven students were recruited. Mean compression rates did not differ between the 3 groups. However, the mean percentage of adequate compressions in the CCO group was significantly lower than that of the 15:1 or 30:2 group (31.2±30.3% vs. 55.1±37.5% vs. 54.0±36.9%, respectively; P<0.001) and the difference occurred within the first minute. The RPE score in each minute and heart rate change in the CCO group was significantly higher than those of the C:V ratio groups. There was no significant difference in maximal oxygen uptake between the 3 groups. Energy expenditure in the CCO group was relatively lower than that of the 2 C:V ratio groups. Conclusion CPR using a 15:1 C:V ratio may provide a compression quality and exercise intensity comparable to those obtained using a 30:2 C:V ratio. An earlier decrease in compression quality and increase in RPE and heart rate could be produced by CCO CPR compared with 15:1 or 30:2 C:V ratios with relatively lower oxygen uptake and energy expenditure.

Hypoglycemic and hypolipidemic effects of samnamul (shoot of Aruncus dioicus var. kamtschaticus Hara) in mice fed a high-fat/ high-sucrose diet

김정인,윤정아,정유경,백희진 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.5

The hypoglycemic and hypolipidemic effects of samnamul were investigated. The a-glucosidase inhibitory activity of samnamul in vivo was determined in normal mice. Oral administration of samnamul extract (500 mg/ kg) or acarbose (50 mg/kg) significantly reduced the postprandial glucose response. The effects of chronic consumption of samnamul on fasting hyperglycemia and dyslipidemia were determined in C57BL/6 J mice with diabetes mellitus induced by a high-fat/high-sucrose (HFHS) diet. Consumption of samnamul extract at 0.5% of the diet for 12 weeks decreased serum glucose, triglyceride, and cholesterol levels, the homeostasis model assessment for insulin resistance index, and activities of maltase and sucrase in the small intestine. These results suggest that samnamul had hypoglycemic and hypolipidemic effects in an animal model of type 2 diabetes and that the hypoglycemic effect occurred partly via the inhibition of a-glucosidase activity.

일부지역 직장건강검진 대상자들의 감기에 대한 이해

신새론,최지호,주규진,백희진 대한가정의학회 2010 Korean Journal of Family Medicine Vol.31 No.7

Background: To collect basic data which of conceptions about cold by age, sex, education state, economic state, this survey was carried out in a workers' periodic health examination. Methods: 1,056 workers who are working at the one company were included for this survey from Jun 1 to July 7 2006. We obtained self-reported questionnaire from them. The contents of the questionnaire were the general characteristics of workers, the cause and the management of cold. We analyzed true recognition ratio of conception about cold by age, sex,education state, and economic state. Results: According to sex and economic state, true recognition ratio was not different. According to age, 'Virus', 'Germ','Cold weather' were lowest above fifties about the cause of cold. ‘Self-limited disease’, ‘Flu vaccination prevents cold’,‘Percussion to chest is helpful to remove sputum’, ‘Inhaling warm steam is helpful to decrease nasal obstruction’, ‘Washing hands is helpful to prevent cold’, ‘Many people crowd in a area are susceptible to cold’ were lowest above fifties about the management of cold. According to education state, 'Virus', 'Food' were highest above fifties. Except ‘Medicine cures fast’, ‘Injection therapy cures fast’, ‘Tonsillectomy prevents cold’, the others were highest in the above college education. According to education state by age group, only 'Virus' in the forties and 'Virus, 'Food' in the above fifties were highest. 'Flu vaccination prevents cold' in the twenties, 'Injection therapy cures fast' in the forties, 'Medicine cures fast' and 'Injection therapy cures fast' were lowest in the above college education. Conclusion: Age, education level were significantly related to true recognition ratio of conception about cold. So we had to educate low recognition ratio of conceptions. 연구배경: 인구학적 특성에 따른 감기에 대한 이해정도를 파악하여 보다 비중을 둬야 할 환자교육 기초자료를 얻고자 시행하였다. 방법: 2006년 6월 1일부터 7월 7일까지 일개 의료센터에서 검진을 받는 1,178명을 대상으로 하였다. 회수된 설문지 중 분석이 가능하였던 1,056부를 분석하였다. 설문지는 대상자들의일반적 특성과 감기의 원인, 치료에 관한 내용이며 성별, 연령,교육수준, 경제수준에 따른 감기에 대한 이해정도를 분석하였다. 결과: 성별, 소득수준에 따른 감기개념의 옳은 인식률의 차이는 없었다. 연령대에 따른 감기의 원인에 대한 질문들에서 ‘바이러스’, ‘세균’, ‘추운 날씨’ 모두 50대 이상에서 옳은 인식률은가장 낮았고 치료에 대한 질문들에서 ‘스스로 나을 수 있는 질환이다’, ‘독감 예방접종은 감기를 예방한다’, ‘편도절제술은감기를 예방한다’, ‘가슴을 두들겨 주는 것은 가래제거에 도움이 된다’, ‘뜨거운 증기를 들이마시는 것은 코막힘 증상에 도움이 된다’, ‘손씻기는 감기예방에 도움이 된다’, ‘사람이 많은 곳은 감기가 전염되기 쉽다' 등에서는 모두 50대 이상에서 낮았다. 교육수준에 따른 분류에서는 원인에서는 ‘예’라고 응답한비율이 차이가 없었던 ‘세균’, ‘추운 날씨’, 치료에서는 ‘독감예방접종은 감기를 예방한다’, ‘따뜻한 물의 섭취는 가래제거에도움이 된다’, ‘사람이 많은 곳은 감기가 전염되기 쉽다’는 질문들을 제외한 나머지 질문들에서 옳은 인식률이 대학교졸업이상에서 높았다. 연령대별로 나눈 후 교육수준별로 분석했을 때는 원인에서는 40대에서는 ‘바이러스’만 50대 이상에서는 ‘바이러스’, ‘음식’에서 대학교졸업 이상에서 높았고 20대에서는 ‘독감예방접종은 감기를 예방한다’, 40대에서는 ‘주사를맞으면 빨리 낫는다’만이 50대 이상에서는 ‘약을 먹어야 빨리낫는다’, ‘주사를 맞으면 빨리 낫는다’ 들에서만 대학교졸업 이상에서 낮았다. 결론: 옳은 인식률이 차이가 있는 인구학적 특성은 연령, 교육수준이었고 옳은 인식률이 낮은 개념들에 대해 보다 중점적으로 교육해야 하겠다.

Violacein을 생산하는 Massilia sp. EP15224 균주

윤상홍 ( Sang Hong Yoon ),백희진 ( Hee Jin Baek ),권순우 ( Soon Wu Kwon ),이창묵 ( Chang Muk Lee ),심준수 ( Joon Soo Sim ),한범수 ( Bum Soo Hahn ),구본성 ( Bon Sung Koo ) 한국미생물생명공학회(구 한국산업미생물학회) 2014 한국미생물·생명공학회지 Vol.42 No.4

비올라세인은 항균, 항암, 항산화, 항말라리아, 항설사 활성과 같은 다양한 생리활성을 가지는 보라색소 물질로 알려져 있다. 본 연구에서는 한국의 산림토양에서 분리되었으며 보라색 색소를 생산하는 EP15224 균주를 선발하여 16S rRNA유전자의 염기서열을 분석하였다. 그 결과 Massilia sp. BS-1과 97%의 가장 높은상동성을 보였다. 또한 16S rRNA 유전자에 근거한 계통분류학적 분석에서 EP15224는 기존 보고된 비올라세인 생산 세균들과는 별개로 구분되는 Massilia속의 새로운 종임을 확인하였다. 이 균주가 생산하는 보라색소물질을 분리하여 LC/MS/MS로 분석한 결과, 분자량이 343.34인 비올라세인과 일치하였다. 또한 비올라세인의 생산효율을 높여주는 최적의 배지성분[glucose 2 g/l, (NH4)2SO4 1 g/l, Na2HPO4· 7H2O 2 g/l, KH2PO4 1 g/l, MgSO4· 7H2O0.1 g/l, L-tryptophan 0.24 g/l]과 배양조건[25oC에서 72시간배양, 250 rpm, 10% 접종량]을 조사하였으며 이 조건에서 액체배양 하였을 때 리터 당 280 mg의 비올라세인이 생산되었다. Violacein has received much attention due to its various important biological activities, including broad-spectrum antibacterial and antifungal activity, anti-malarial, anti-tumoral, anti-oxidant, and anti-diarrheal activities. EP15224 strain isolated from forest soils in Korea was found to be a new species belonged to the genus Massilia based on its 16S ribosomal DNA sequences. The 16S ribosomal DNA of strain EP15224 displayed 97% homology with Massilia sp. BS-1, the nearest violacein-producing bacterium. Strain EP15224 produced bluish-purple pigment well in a synthetic MM2 medium containing glucose, (NH4)2SO4, Na2HPO4·7H2O, KH2PO4, MgSO4·7H2O, and 1 mM L-tryptophan. The chemical analysis of the pigment by LC/MS/MS showed that it is violacein with molecular weight of 343.34. This is the second report on the production of violacein by a Massilia species. In this study, the optimal culture conditions for violacein production were established under which 280 mg/l crude violacein was produced : glucose 2 g/l, (NH4)2SO4 1 g/l, Na2HPO4·7H2O 2 g/l, KH2PO4 1 g/l, MgSO4·7H2O 0.1 g/l, L-tryptophan 0.24 g/l, 25 ml medium in a 250 ml flask, with an inoculumn size of 10% (v/v), 72 h of cultivation with 250 rpm at 25oC.

국내 외래 양서·파충류 인공 번식 현황

구교성 ( Kyo Soung Koo ),김유진 ( Eugene Kim ),백희진 ( Hee Jin Baek ),서희정 ( Hui Jeong Seo ),황영혜 ( Young Hae Hwang ),장이권 ( Yikweon Jang ) 한국환경생태학회 2021 한국환경생태학회 학술대회지 Vol.2021 No.2

In the late 1990s, American bullfrogs and red-eared sliders, imported for food, pets, and religious events in South Korea. By natural or artificial factors, however the non-native species were introduced into nature. The Korean government designated the two species as ecosystem disruptors, and subsequently banned imports and transactions because of the risk to the nature. Unfortunately, with the recent expansion of the pet market, more diverse non-native reptiles are being imported into the country. This increase in imports of non-native organisms is linked to an increase in potential inflows to ecosystem and confounding threats. A recent survey found that nearly 20 species of non-native amphibian-reptiles discovered in the wild. Furthermore non-native amphibian-reptiles are actively bred by people for hobby or profit reason, but it has not been determined how many amphibians and reptiles are artificially bred in South Korea. Therefore in this study, we investigated how various non-native amphibian-reptiles are subject to artificial reproduction. For the collection of data, all non-native amphibian-reptiles were collected from 1st to 31st August 2021. We used searching engine such as Google, YouTube, Naver, and Daum to find breeding recorded. Keywords that can be related to artificial breeding such as "cultivation, breeding, non-native species, and rare species were used to gain accurate data. In the collected data, it was divided into 1) breeding period, 2) breeding location, 3) breeding target species, 4) success or failure of breeding, and 5) breeding subjects. We found 368 cases of artificial breeding. The first case was recorded in 2008 with Burmese python (1 case) and have since increased steadily until 2021. The most of breeding recorded in 2021, with 117 (31.7%) cases. Artificial breeding took place at a total of 146 locations, Gyeonggi-do has the most abundant site of 57 locations, followed by Seoul has 30 locations, Chungnam has 15 locations, Incheon has 10 locations and Gyeongbuk has 8 locations. The breeding population (n=368) had the largest number of 126 lizard cases (34.2%) with 94 snakes, 92 turtles, 54 frogs and 2 salamander cases. There was a total of 129 non-native amphibian-reptiles that were subject to reproduction. Among them, Leopard Gecko was 42 cases followed by corn snake 33, king snake 17, and ball python 17. From 350 cases that identified the breeding results, 75.4% (264) were successful (hatching). 226 cases of breeding were conducted by individuals this is twice as many as 113 cases by companies. The breeding of non-native amphibian-reptiles tended to be concentrated mainly in Seoul and Gyeonggi-do, the metropolitan area. A total of 677 species of non-native amphibian-reptiles were announced in 2019, 19.1% of which were subject to artificial breeding. Among the various non-native amphibian-reptiles, Leopard gecko had the highest frequency of reproduction, which can be related to various factors such as low breeding difficulty, high selling price, and high reproduction rate. A risk assessment of the top 10 species including Leopard gecko will need to be made for preparing the potential introduction into nature. Considering the more cases of individual breeding than businesses and the success rate of breeding exceeds 70%, there is a possibility that the number of non-native amphibian-reptiles will increase rapidly in the future. Therefore management and safeguards for artificial reproduction by individuals and companies must be prepared.

당근식초의 췌장 라이페이스 저해활성 및 항산화 활성

김정인(Jung-In Kim),한도원(Do-Won Han),윤정아(Jung-A Yun),백희진(Hee-Jin Baek),임상욱(Sang-Wook Lim) 한국생물공학회 2018 KSBB Journal Vol.33 No.2

The purpose of this study was to investigate pancreatic lipase inhibitory activities and antioxidant activities of carrot juice and fermented carrot vinegar in vitro. Carrot juice was prepared using a low-speed masticating juicer. Carrot juice was fortified with pear extract to 24 obrix, and then alcoholic fermentation was conducted using Saccharomyces cerevisiae (EC-1118) (3%) at 30℃. The alcohol fermentation product was fermented using Acetobacter pasteurianus (KCCM 12654) (5%) at 25℃ to produce carrot vinegar. Pancreatic lipase inhibitory activity and DPPH radical scavenging activity of carrot vinegar were 4.1 and 4.6 times higher than that of carrot juice, respectively (p<0.01). Total polyphenol contents of carrot juice and vinegar were 8.6 and 18.1 mg TAE/100 mL, respectively. Flavonoid contents of carrot juice and vinegar were 1.0 and 10.5 mg CTE/100 mL, respectively. The result demonstrated that fermented carrot vinegar could be a possible candidate for anti-obesity agent.