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배진주(Jinjoo Bae),이선이(Sun-Yi Lee),송재영(Jae-Young Song),이정로(Jung-Ro Lee),윤문섭(Munsup Yoon),이정윤(Jung-Yoon Yi),김행훈(Haeng-Hoon Kim),이영이(Young-Yi Lee) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.6
Cryopreservation method using a droplet vitrification was applied to the thirty-one strawberry accessions of in vitro grown shoot tips. A protocol with 0.3 - 0.5 M preculture followed by C4 loading and B1 dehydration solutions efficiently implemented cryopreservation of twenty-six strawberry accessions. The highest regrowth rate was 85.8% for PHS0007 and others were ranged between 85.8% and 21.0%. A slightly modified protocol was applied to five accessions. With these two protocols, twenty-eight accessions obtained more than 40% regrowth rate. This study showed that the droplet vitrification method was able to practically implement cryopreservation of in vitro grown shoot tips of broad range of strawberry germplasm (105).