수종의 광독성 약물투여 후 시간에 따른 피부농도 및 최소 광독량에 관한 연구

박윤기,조무연,한승경,임성빈 ( Yoon Kee Park,Moo Yon Cho,Seung Kyung Hann,Sung Bin Im ) 대한피부과학회 1991 大韓皮膚科學會誌 Vol.29 No.5

N/A The Skin Concentration and Minimal Phototoxic Dose Following Administration of Phototoxic Drugs as a Function of Time Yoon-Kee Park, M.D., Moo Yon Cho, M.D., Saung Kyung Hann, M.D., Sungbin im, M.D. Department of Dermatology Yonsei Univeraity College of Medicine, Seoul, Korea Psoralen photochemotherapy(PUVA) is defined as a treatment that depends on the interaction of a photosensitizing substance, psoralen, and long wave jltraviolet(UVA) radiation that results in therapeutically beneficial effects. The rationale of its use is to clear skin disease by repeated controlled phototoxic reactions. The efficacy of PUVA depends on the drug, the UVA dose and wave length, the individual sensitivity to the phototoxic reaction and on the disease to be treated. However, the major factor is the amount of psoralen at the site of photochemical reaction in skin. Daspite the extensive experiences which have now accumulated in the use of PUVA, the relationship between the therapeutic response and phototoxicity as it occurs in normal skin is not established. Comparative data on different psoralen molecules and their skin sensitizing ability are required. From studies on the skin concentration using HPLC and minimal phototoxic dose following oral administration, intraperitoneal administration, a bath of phototoxic drugs as a function of time in guinea pigs, the following results are obtained. 1. The skin concentration of phototoxic drugs after oral administration peaked at 1.5 hours, and the concentration of 8-methoxypsoralen(8-MOP) was 3.7 times greater than that of 5-methoxypsoralen(5-MOP). The skin concetration of 4,5,8-trimethylpsoralen(TMP) was not detected in this study(limit of sensitivity 5ng/g). 2. The skin conentration of phototoxic drugs after intraperitoneal administration peaked at 30 minutes, and the concentration of 8-MOP was slightly greater than that of 5-MOP. The skin concentration of TMP was not detected in this study(limit of sensitivity 5ng/g). 3. The skin concentration of phototoxic drugs after bathing peaked immediately after bathing, and the skin concentrations of phototoxic drugs after bathing decreased in the order of 5-MOP, TMP, and 8-MOP. 4. The minimal phototoxic dose of phototoxic drugs after oral administration was lowest at 1.5 hours, and the minimal phototoxic dose of 8-MOP was 8 times less than 5-MOP, 24 times less than TMP. The phototoxicity of 8-Mop after oral administration was strongest. The time of lowest phototoxic dose and highest skin concentration was the same. 5. The minimal phototoxic dose of phototoxic drugs after intraperitoneal administration was lowest at 30 minutes, and the minimal phototoxic dose of 8-MOP was 1.3 times less than 5-MOP, 5.7 times less than TMP. The phototoxicity of 8-MOP after intraperitoneal administration was strongest. The time of lowest phototoxic dose and highest skin concentration was same. 6. The minimal phototoxic dose of phototoxic drugs after bathing was lowest at 15 minutes. The minimal phototoxic dose of 8-MOP was not significantly different but was significantly greater than that of 5-MOP.

C3H 생쥐에서 자외선 A조사 후 표피내 Langerhans세포의 회복에 관한 연구

박윤기(Yoon Kee Park),김수민(Soo Min Kim),한승경(Seung Kyung Hann),조무연(Moo Yon Cho) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.3

This study was undertaken to investigate the recovery of epidermal Langerhans cells in relation to time after UVA irradiation through different amounts and ways of exposure in CH mice. We irradiated the ears of C2H mice with UVA 200J/cm2 and 400J/cm2 in a single dose at one time or 5 fractionated doses for 5 days and performed biopsies on the ears of the control and experimental groups after 2, 7, 14, 21days of irradiation and stained them with immunoperoxidase method. The results are summarized as follows, l. We observed a significant decrease in the number of the Ia-positive epidermal Langerhans cells in the single-dose-exposed group compared to the fractionated- dose-exposed group on 7th and 14th days irradiated with UVA 200J/cm. 2. There was no significant difference in the change in the number of the Ia- positive epidermal Langerhans cells until 21 days of exposure between the single- dose-exposed group and the fractionated-dose-exposed group irradiated with UVA 400 J/cm 3. In the group irradiated with UVA 2003/cm, the reduced number of the Ia-positive epidermal Langerhans cells returned to normal on the 14th day after irradiation in the fractionated-dose-exposed group and on the 21st day in the single- dose-exposed group. In the group irradiated with 400J/cm, the number returned to normal on the 21st day of irradition both in the fractionated-dose-exposed group and in the single-dose-exposed group. (Kor J Dermatol 28(3): 261 268, 1990)

백반증 환자에서 항멜라닌세포 자가항체 활성도의 변화에 관한 연구 - 시험관내 보체매개 멜라닌세포 독성능의 측정 -

박윤기(Yoon Kee Park),한승경(Seung Kyung Hann),송민석(Min Seok Song),윤정구(Jung Ku Yoon),김형일(Hyung Il Kim) 대한피부과학회 1991 大韓皮膚科學會誌 Vol.29 No.3

Several observation suggest that the antimelanocyte autoantibodies could play a role in melanocyte destruction. Some experiments indicate that melanocyte antibodies from patients with vitiligo can kill melanocyte in vitro. In these experiments, we demonstrated that vitiligo patient's sera containing antimelanocyte antibodies can lyse cultured human melanocytes by complement activation. Melanocyte cytotoxicity was measured using the ethidium bromide/ acridine orange viability assay. Significant melanocyte cytotoxicity was seen in sera from patients with both active and inactive vitiligo(p<0.01). Melanocyte cytotoxicity measured with complement-mediated cytotoxicity decreased after systemic steroid treatment(p<0.05) ; however melanocyte cytotoxicity showed no significant change with systemic PUVA therapy. (Kor J Dermetol 20(3): 391 3081, 1991)

건선 치료에 있어서 PUVA와 Etretinate 의 병용 치료법에 대한 임상적 고찰

박윤기(oon Kee Park),한승경(Seung Kyung Hann),홍경태(Kyung Tai Hong) 대한피부과학회 1987 대한피부과학회지 Vol.25 No.4

RePUVA therapy is the combination of an oral aromatic retinoid(etretinate) with oral photochemotherapy (PUVA) for the treatment of psoriasis. This combination therapy has the advantage of faster clearance with fewer side effects. Twenty three ambulatory psoriasis patients received RePUVA treatment. Etretinate was administered in a dosage of approxirnately 0.75 mg/kg body weight for 2 weeks and discontinued. One week after discontinuance of etretinate, PUVA therapy was begun. Complete clearance occured in 96% of the patients with an average of 9.5+4 irradiations and an accumulated ultraviolet A (UVA) dose of 67.5+42 J/cm. The primary side effects were dry rnucous membranes and mild cheilitis. Laboratory findings revealed no significant abnormalities before or after etretinate treatment.

백반증 환자에서 표피 Langerhans 세포수의 변화에 관한 연구

박윤기(Yoon Kee Park),한승경(Seung Kyung Hann) 대한피부과학회 1987 대한피부과학회지 Vol.25 No.4

Report. about numeric change of LC between vitiliginous skin(VS) and adjacent normal appering skin (ANAS) are hard to find, Epidermal Langerhans Cell (LC) dersities in VS and ANAS were studied in 18 patient with generalized vitiligo. Adenosine triphosphatase(ATPase) stain was used to characterize LC. Epidermal LC densities were calculated by means of an eye piece reticule and expressed per mm. The results were as follows; 1. The was significant body site variation of LC densities in ANAS and the range of densities of LC per mm were from 668+165 to 1,241 _3 and the mean density of LC per mm was 944+258. 3. The LC densities of VS was similar to that of ANAS(p: not significant). In conclusion quantiative change of epidermal LC in vitiligo doesn't seem to have significant relation with pathogenesis of vitiligo.

Indomethacin 투여가 마우스의 접촉 과민 반응에 미치는 영향

한승경(Seung Kyung Hann),박윤기(Yoon Kee Park),김형일(Hyung Il Kim),최응호(Eung Ho Choi) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.3

To study the effect of indomethacin treatment on the reactivity of contact hypersensitivity and discuss relevant mediators which could affect contact hypersensitivity, the following items were evaluated: the change in the plasma concentration of prostsglandin E following indomethacin treatment; the change of contact hypersensitivity following indomethacin treatment. The results ore summarized as follows: 1. The plasma level of prostaglandin E in indomethacin treated mice decreased. 2. The contact hypersensitivity of indomethacin treated mice was depressed. Considering the suppression of contact hyper sensitivity by indomethacin treatment, the metabolites of cyclo-oxygenase such as prostaglandin E may be the possible mediators of induction of contact hypersensitivity. (Kor J Dermatol 28(3): 278 282, 1990)

전신성 백반증 환자 174예의 임상적 고찰

한승경(Seung Kyung Hann),박윤기(Yoon Kee Park),황규천(Kyu Chun Whang),김형주(Hyung Joo Kim) 대한피부과학회 1986 大韓皮膚科學會誌 Vol.24 No.6

This is an analysis of 174 patients classified as generalized vitiligo by Ortonnes' classification during the period from May till December in 1985. The retults are summarized as follows. There were 89 females(51.l%) and 85 males(48, 9%). 2. The mean age of onset was 24.2 years(male: 23.4, female: 24. 8). 3. The mean age at the first visit was 33 years(male: 32. 2, female: 34, 8) 4. The mean duration of t.he disease was 10. 8 years (male: 10. 3, female: 11. 3). 5. The mean interval between onset and visiting was 9 years(male: 8. 8, female: 10). 6. Mode of onset was unicentric in 90, 2% and multicentric in 9. 8% 7.The most common site of initial involvement was abdomen(15. 0%). 8. The most common precipitating factor was trauma(11%). 9.The most common previous treatment was sunlight irradiation after application of topical oxoralene(33.3%). 10. The most common degree of depigmented lesion was 5~10%.(39.7%). 1l. Family history was present in 12% and thyroid disease was associated in only one case.

지속성 이색성 홍반 25예의 임상적 연구

이승헌(Seung Hun Lee),한승경(Seung Kyung Hann),박윤기(Yoon Kee Park),이성낙(Sung Nack Lee) 대한피부과학회 1986 大韓皮膚科學會誌 Vol.24 No.6

We studied 25 cases of clinically diagnosed erythema dyschrornicum perstans (EDP) who were seen in our clinic within 11-year period, from 1975 through 1985, and compared light and electron microscopic findings, and direct immunoflunrescence findings. The results are summarized as follows: 1. There were 16 males(64%) and 9 females(36%). The peak age incidence was in the second decade. 2. The mean age of onset was 18 years(M: 16. 6, F: 19. 4) and the rnean duration was 1.8 years(M: 2.6, F: 1.0). 3. The annual incidence increased from 1981 and was peaked in 1985. 4. The most commonly involvement site was the trunk(92%), the least common involvement(4%) was one of the face, neck, and shoulder combined. 5. The histopathologic findings in most patients shows focal vacuolization of the basal cell layer, pigment incontinence, dermal melanophages. 6. Electron microscopic findings of five patients consist of vauoles with abundant melanosomes in the basal cells, widened intercellular spaces and dermal melanophages.

지속성 광선반응증

박경득(Kyung Deuk Park),임성빈(Sung Bin Im),한승경(Seung Kyung Hann),박윤기(Yoon Kee Park) 대한피부과학회 1992 대한피부과학회지 Vol.30 No.6

Persistent light reaction is a condition of chronic photodermatitis in which photosensitive reaction persists even after the rernoval of all photosensilizers. A 56-year-old man had experienced a recurrent dermatitis involving primarily the face, neek, forearms and hands for 9 years, this condition was aggravated by sunexposure. Photopatch testing disclosed a strongly positive reaction to chloropromazine, promethazine, acid trichlorocarbanilide, Phototesting also revealed lowered MED with UVA and UVB thar norrmal mean value. (Kor J Dermatd 1992;30(8):901-905)

Chronic Actinic Dermatitis Showing a Good Response to Cyclosporine

한승경 ( Seung Kyung Hann ),조흔정 ( Heun Jung Cho ),이광훈 ( Kwang Hoon Lee ),박윤기 ( Yoon Kee Park ) 대한피부과학회 1996 초록집 Vol.34 No.10