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Electrophoretic pattern of seed proteins of perilla
수실런(K. N. Suseelan),방진기(Jin Ki Bang),미트라(R. Mitra) 한국육종학회 1996 한국육종학회지 Vol.28 No.4
Total seed proteins of 26 accessions of perilla were analysed by SDS-PAGE. The major polypeptides were separated into three zones. Zone 1 consisted of a single polypeptide of molecular weight of 47.8 kDa, while Zone 2 had four polypeptides of 31.6, 30.9, 30.2 and 29.5 kDa and Zone 3 showed three polypeptides of 22.3, 21.8 and 20.9 kDa. SDS-PAGE of unreduced proteins demonstrated that the polypeptides of Zone 2 and 3 were the products of larger polypeptides with S-S linkage. A polypeptide of 27.5 kDa was resolved between Zone 2 and Zone 3 and appeared to be single polypeptide component. A radiation-induced mutant did not show this polypeptide. All the accessions had similar electrophoretic pattern suggesting a narrow genetic base of the accessions. The perilla seed protein pattern has similarity with legumin and vicilin-like proteins, usually present in legumes.
A rapid and simple technique for determination of α-linolenic acid by spot test in perilla seeds
방진기(Jin Ki Bang),바이댜(Umesh. J. Vaidya),크리쉬나(T.G. Krishna),미트라(R. Mitra) 한국육종학회 1996 한국육종학회지 Vol.28 No.4
The objective of this study was to establish the effective use of spot test for early selection of perilla mutants which have low α-linolenic acid in seed oil. The average α-linolenic acid content with 30 accessions determined by GLC was 57.9% and the contents ranged from 51.4 to 65.6%. The results of spot test, red colour is related to high α-linolenic acid content in perilla whereas yellow colour indicates absence of α-linolenic acid. The colour density by an image analysis was highest in perilla among oil crops tested. The correlation coefficient between colour density by spot test and α-linolenic acid content determined by GLC was highly significant. Therefore, the spot test was considered useful as a preliminary screening method for the relative α-linolenic acid content in mutation breeding.