노인복지시설의 미용프로그램 현황에 관한 연구

문정혜(Jung-Hye Moon),도주연(Joo-Yun Do) 한국인체미용예술학회 2013 한국인체미용예술학회지 Vol.14 No.2

Recently, life expectancy in Korea has increased along with economic growth. As the demand for a better life increases rather than just making ends meet, people have become more interested in new employment, continuing education and high-quality leisure life as well as in economy and leisure activities. As we get older, however, it is very hard to avoid senile diseases along with economic difficulties. The healthy aged may have a chance totravel around or further their education and get a new job. If they are sick, however, they will not be given these opportunities. Thanks to the long-termcare insurance system which was launched in 2008, the aged have been able toget the care they’ve needed at a welfare facility for the elderly, community facility, or at home. People want to spend a peaceful and beautiful life in the later chapters of their lives. However, a beauty care program has not been familiar to the aged who have received medical care anduse elderly care facilities. In fact, volunteer services have been given by mostly volunteer hair dressers. As a result, it hasn’t been common for the aged to participate in the program and benefit from the therapeutic welfare program. In reality, it is necessary to perform more specified demonstration studies to promote the beauty welfare psycho-therapy program for the aged.

군소 (Aplysia kurodai)의 중추신경계로부터 myomodulin A와 E의 정제

김찬희,서해점,황은영,김은정,고혜진,김인혜,서정길,문정혜,허민도,박남규,KIM Chan-Hee,SEO Hae Jeom,HWANG Eun-Young,KIM Eun Jung,GO Hye-Jin,KIM In Hye,Seo Jung-Kil,Moon Jung-Hye,HUH Min-Do,PARK Nam Gyu 한국수산과학회 2001 한국수산과학회지 Vol.34 No.3

군소의 중추신경계로부터 myomodulin A (MMA)과 myomodlin E (MME)의 정제에 대해 보고하고자 한다. 500마리 군소의 중추신경계를 산조건으로 추출한 후 4종류의 HPLC column system에 적용하여 두가지 신경성 펩타이드를 정제하였다. HPLC 분리과정에서 얻어진 모든 분획의 활성을 조사하기 위하여 bioassay system으로 진주담치 (Mytilis edulis)의 ABRM을 이용하였다. 최종 정제된 신경성 펩타이드는 Edman 분해법을 이용한 아미노산 서열 분석기, 질량분석기와 화학합성을 통해 완전한 일차구조를 밝힐 수 있었다. 정제한 MMA와 MME의 일차구조는 각각 Pro-Met-Ser-Met-Leu-Arg-Leu-$NH_2$, (847.41 Da) 과 Gly-Leu-Gln-Met-Leu-Arg-Leu-$NH_2$, (830.50Da) 이었다. 또한 이들 합성 펩타이드는 진주담치의 ABRM에 대해 phasic contration의 조절활성을 나타내었다. This paper reports the purification of myomodulin A (MMA) and myomodulin E (MME) from the sea hare. The central nervous systems of 500 sea hare were extracted in an acidified solvent, after which four HPLC column systems were used to obtain pure peptides, The phasic contraction bioassay using a Mytilus edulis anterior byssus retractor muscle (ABRW) was applied to monitor all collected fractions. The pure peptides were submitted to Edman degradation based automated microsequencing. Mass spectrometry and chemical synthesis confirmed the sequence. The primary structures of MMA and MME were Pro-Met-Ser-Met-Leu-Arg-Leu-$NH_2$, (847.41 Da) and Cly-Leu-Gln-Met-Leu-Arg-Leu-$NH_2$, (830.50 Da), respectively. Synthetic peptides showed a modulating activity of phasic contraction in the ABRM of Mrtilus edulis.

20대 여성의 화장 행동과 화장품 선호도 분석

김칠순 ( Chil Soon Kim ),문정혜 ( Jung Hye Moon ) 대한가정학회 2005 Human Ecology Research(HER) Vol.43 No.11

열연 공정에서의 영상을 이용한 캠버 및 최적 절단선 검출 알고리즘

공남웅(Nam Woong Kong),문정혜(Jung Hye Moon),박부견(PooGyeon Park) 대한전기학회 2007 대한전기학회 학술대회 논문집 Vol.2007 No.10

This paper presents the vision-based camber and optimal cutting line detection algorithm for hot-rolling process. It is important to measure the camber of head and tail part of strips because many problems are caused by the camber in the hot-rolling process. The hot-rolling process has time constraints. The camber detection algorithm of head and tail parts requires fast and less complex for satisfying time constraints. The proposed algorithm consists of two parts: measurement of the camber in the head and tail part of strips and decision part of the optimal cutting line of hot-rolled strip. First, we obtain the camber value of the strip from the difference between the real center line and the center line of head, tail part. Second, the head and tail part of strips isn't suitable for strips connections. Therefore, the cutting process is needed in the hot-rolling process. The optimal cutting line is determined by the head and tail images obtained from cameras. The algorithm is applied into the vision system with two area cameras, Matrox image processing board and host PC for verification.

직장여성과 여대생의 화장품 선호도 및 구매행동에 관한 연구

김칠순(Chil-Soon Kim),문정혜(Jung-Hye Moon) 한국의상디자인학회 2004 한국의상디자인학회지 Vol.6 No.2

Conditions of Quantitative Analysis for Free Amino Acid in Fermented Proteins

류홍수(Hong-Soo Ryu),문정혜(Jung-Hye Moon),이강호(Kang-Ho Lee) 한국식품영양과학회 1988 한국식품영양과학회지 Vol.17 No.2

발효단백질 중의 유리아미노산을 효과적으로 정량하기 위하여 정량방법(추출조건, 除蛋白 및 발색법)에 따른 영향을 검토하였다. 낙농발효품의 수용성 유리아미노산은 75℃, 40분, 대두발효품은 40℃, 3시간에서 효과적으로 추출되었다. 여러 침전방법(95% ethanol 처리, 100℃, 3분 가열 및 1% picric acid phthaldialdehyde)로 발색시켜 D.L.-lysine을 표준아미노산으로 삼아 정량, 계산하였을 때가 아미노산자동분석기에 의한 결과에 가장 접근하였으나 ethanol로 침전시킨 뒤 OPDA로 발색시킨 결과도 이와 유사하였다. TNBS(2,4,6-trinitrobenzensulfonic acid)에 의한 발색정량에는 ethanol 침전처리가 가장 효과적이었으나 종래의 銅監法(copper salt method, Spies and Chamber, 1958.)에 의한 결과는 다른 비색정량 및 아미노산자동분석기에 의한 결과에 비하여 시료에 따른 과소의 차이가 심하였고 재현성이 결여되었다. This study was performed to provide the optimal conditions of quantitative analysis for free amino acid in fermented protein foods. The water extractable free amino acid from dairy fermented foods was extracted effectively at 75℃ for 40 min., while it were extracted from fermented soy products at 40℃ for 3 hours. A close results of free amino acid content to those from amino acid analyzer were obtained using OPDA method with lysine standard after deproteinizing with 1% picric acid. 95% ethanol used as a deproteinizing reagent could give a comparable results to those from picric acid treatment in determining free amino acid content using OPDA method. Therefore, ethanol treatment was more recommendable than picric acid treatment which has some troubles in removing excess picric acid through Dowex resin column. The most desirable precipitation method for free amino acid determination using TNBS method was 95% ethanol treatment among the various deproteinizing procedure. The copper salt method was not suitable owing to its lacking reproducibility and pronounced discrepancy in determining free amino acid.

홍삼에탄올추출물의 염증유발인자에 대한 억제효과

최경민,황승미,임지예,고은실,박종혁,문정혜,이민정,장지은,차정단,Choi, Kyung-Min,Hwang, Seung-Mi,Lim, Ji-Ye,Ko, Eun-Sil,Park, Jong-Hyuk,Moon, Jung-Hye,Lee, Min-Jung,Jang, Ji-Eun,Cha, Jeong-Dan 한국미생물·생명공학회 2015 한국미생물·생명공학회지 Vol.43 No.2

In this study, we investigated the effects of 40% ethanol extract of Red Ginseng (RGE) on the productions of inflammatory proteins in Antigen I/II (Ag I/II)-N, a recombinant protein isolated from Streptococcus mutans -stimulated in RAW 264.7 cells. RGE inhibited the expression of Ag I/II-N-induced pro-inflammatory mediators, both mRNA and protein synthesis levels, without any cytotoxic effects. Moreover, RGE significantly inhibited Ag I/II-N induced NF-κB translocation into the nucleus by preventing the degradation of inhibitor κB-α. In conclusion, RGE down regulates the expression of pro-inflammatory genes involved in the synthesis of NO and iNOS in Ag I/II-N-stimulated RAW 264.7 cells by suppressing NF-κB activity. 본 연구에서, 우리는 S. mutans Ag I/II 재조합단백질에 의해 유도되어진 염증유발 단백질의 발현에 홍삼 40% 에탄올 추출물의 효과를 알아보고자 하였다. 홍삼에탄올추출물은 Ag I/II 재조합단백질에 의해 유도되어진 염증유발물질들의 mRNA와 단백질의 발현을 억제하였다. 더불어 홍삼에탄올 추출물은 NF-κΒ p65가 핵내로 이용하는 것이 억제하였다. 결론적으로 홍삼 40%에탄올추출물은 NF-κB의 활성에 의해 NO 생성과 iNOS 발현이 조절되어지는 것으로 생각되어지며, 염증유발 관련 유전자들의 낮은 발현을 유도하는 것으로 관찰되어졌다.

Erratum to: 홍삼에탄올추출물의 염증유발인자에 대한 억제효과 (Red Ginseng Ethanol Extract Suppressed Ag I/II-induced Up-expression ofInflammatory Mediators in RAW 264.7 Macrophages)

최경민,황승미,임지예,고은실,박종혁,문정혜,이민정,장지은,차정단,Choi, Kyung-Min,Hwang, Seung-Mi,Lim, Ji-Ye,Ko, Eun-Sil,Park, Jong-Hyuk,Moon, Jung-Hye,Lee, Min-Jung,Jang, Ji-Eun,Cha, Jeong-Dan 한국미생물 · 생명공학회 2016 한국미생물·생명공학회지 Vol.44 No.2

Bowfin-과 Shark-neuropeptide $\gamma$의 구조 및 수축효과

김은정,서정길,김찬희,고혜진,허민도,문정혜,박장수,박남규,KIM Eun Jung,SEO Jung-Kil,KIM Chan-Hee,GO Hye-Jin,HUH Min-Do,MOON Jung-Hye,PARK Jang-Su,PARK Nam Gyu 한국수산과학회 1999 한국수산과학회지 Vol.32 No.2

Tachykinin peptide의 구조와 생리활성간의 상관관계를 조사하기 위해 고상법으로 합성한 어류 유래의 neuropeptide $\gamma$(Mammalian-, Bowfin- 그리고 Shark-neuropeptide $\gamma$)를 사용하였다. 이들의 이차 구조를 알아보기 위해 circular dichroism (CD)을 이용하였다. CD 연구 결과에 따르면, mammalian-neuropeptide $\gamma$, bowfin-neuropeptide $\gamma$와 shark-neuropeptide $\gamma$는 완충액과 인공지질막 조건하에서 random구조를 나타내었다. 또한 이들 neuropeptide $\gamma$의 장관에 대한 수축 활성을 조사하기 위해 guinea-pig의 회장, rat의 십이지장과 carp intestine을 사용하였다. Carp intestine에 서 bowfin-neuropeptide $\gamma$>shark- neuropeptide $\gamma$>mammalian-neuropeptide $\gamma$순으로 활성 이 나타났다. 그러나, guinea-pig 회장과 rat 십이지장에 대해서 mammalian-NP$\gamma$의 활성이 어류 유래의 neuropeptide $\gamma$들보다 높게 나타났다. 이러한 결과들은 neuropeptide $\gamma$가 종-특이적인 활성을 나타냄을 제시한다. The relationship between structure and contractile activity was studied on the three neuropeptide $\gamma$ (mammalian-, bowfin-, shark-NP$\gamma$) that were synthesized by the solid-phase method. Circular dichroism spectra showed that mammalian-, bowfin- and shark-neuropeptide $\gamma$ took an unordered structure in buffer solution and artificial liposome. The intestinal motility response was investigated with guinea-pig ileum, rat duodenum and carp intestine. In case of carp intestine, contractile activity was as follows; bowfin-NP$\gamma$> shark-NP$\gamma$>mammalian-NP$\gamma$, On the other hand, the contractile activity of mammalian-neuropeptide $\gamma$ was more potent than those of bowfin-, shark-neuropeptide $\gamma$ in the guinea-pig ileum and rat duodenum. These results suggest that NP$\gamma$ show the species-specific activity.

개불(Urechis unicinctus)에서 추출한 당단백질의 항암효과 및 면역활성

이종열(Jong-Yeoul Lee),류홍수(Hong-Soo Ryu),문정혜(Jung-Hye Moon),서재수(Jae-Soo Suh) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.28 No.4

개불 당단백질의 sarcom 180 cell 고형암 성장 저지능은 4㎎/㎏에서 43.63%로 나타났으나, 20㎎/㎏이상의 농도에서는 고형암 성장 저지능을 보이지 않았다. Sarcom 180 cell에 대한 수명 연장 실험에서도 2㎎/㎏와 4㎎/㎏의 저농도에서는 상당한 수명연장 효과를 보였으나, 20㎎/㎏ 이상의 고농도 투여군에서는 수명연장 효과를 나타내지 않았다. 개불 당단백질의 sarcoma 180 cell에 대한 직접적인 세포 독성 효과는 농도의 증가에 따라 약간의 종양세포 살해효과를 보였으나, 그 효과는 미약하였다. 개불 당단백질의 면역능 증진효과는 체중에 대한 간과 비장의 중량비 증가, 총 복강세포수의 증가 그리고, 당단백질을 고농도 투여시 30.78%~46.30%정도의 백혈구 수 증가 등으로 확인되었다. 이러한 결과로서 개불에서 추출한 당단백질의 항암효과는 면역활성의 증진과 면역자극의 효과라고 볼 수 있었으나, 전반적인 면역활성의 증가에도 불구하고, sarcoma 180 cell에 대한 고형암 성장 저지실험과 수명연장실험에서 고농도로 투여시에는 오히려 활성이 나타나지 않았다. 따라서 개불 당단백질의 최적투여 농도와 고농도 투여시의 부작용에 관한 더 많은 연구가 필요하였다. 이상의 결과에서 개불에서 추출한 당단백질의 면역자극에 의한 항종양 활성을 확인되므로, 일반적으로 사용되어지는 항암 화학요법제와 더불어 병행될 수 있는 면역증진제로서의 개발 가능성이 있다고 보여진다. To confirm therapeutic functionality of Urechis unicinctus which have been favored as a special seafood in Korea, the antitumor and immunological effect of those glycoprotein were studied. 4㎎/㎏ dose of glycoprotein from Urechis unicinctus was most effective in solid tumor growth inhibition(43.63%) of sarcoma 180 cells. However, in case of mice injected with more than dose of 20㎎/㎏, tumor growth was not inhibited. The higher prolongation ratios were achieved at levels of 2㎎/㎏ with 31.2% and 4㎎/㎏ with 28.9%. The cytotoxic effect of glycoprotein on sarcoma 180 cells was increased slightly as administering level was increased. Number of total peritoneal exudate cells in all the glycoprotein administered groups increased remarkably meaning that Urechis unicinctus gly-coprotein could help to improve immunity. Notable body weight change was not resulted in the glycoprotein treated mice compared with control group, but the ratios of both liver or spleen to body weight were increased in mice injected with 20㎎/㎏ and 40㎎/㎏. These results suggest that the glycoprotein from Urechis unicinctus could stimulate immunity of the mouse bearing tumor cells. Furthermore, the number of leucocytes was also increased by 38.78% at the dose of 20㎎/㎏ and by 46.30% of control at 40㎎/㎏, while the lower level of 2㎎/㎏ or 4㎎/㎏ showed no effect in increasing leucocyte number. The biochemical values such as GOT and GPT in serum were not changed in mice injected with glycoprotein in comparision with control group.