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배양세포에서 전염성조혈장기괴사증 바이러스항원의 면역조직화학적 검출
문운경 ( Oun Kyung Moon ),이민권 ( Min Kwon Lee ),진영배 ( Young Bae Jin ),김순복 ( Soon Bok Kim ) 한국동물위생학회 2002 한국동물위생학회지 (KOJVS) Vol.25 No.3
This experiment was done to set up the immunohistochemical detection method for infectious hematopoietic necrosis virus(IHNV) antigens in the monolayers of CHSE-214 cell cultures inoculated with IHNV. Specific identification of IHNV antigens was detected in the cytoplasms of infected cells by the use of monoclonal antibodies to glycoproteins. The specific positive signal was observed as a distinct red color. The result showed that streptavidin alkaline phosphatase immunohistochemistry specifically identified IHNV antigens in infected cultured cells.
고병원성 조류인플루엔자 (HPAI)의 에어로졸을 통한 공기 전파 예측을 위한 공기유동학적 확산 모델 연구
서일환,이인복,문운경,홍세운,황현섭,권경석,김기연,Seo, Il-Hwan,Lee, In-Bok,Moon, Oun-Kyung,Hong, Se-Woon,Hwnag, Hyun-Seob,Bitog, J.P.,Kwon, Kyeong-Seok,Kim, Ki-Youn 한국농공학회 2011 한국농공학회논문집 Vol.53 No.1
HPAI (High pathogenic avian influenza) which is a disease legally designated as an epidemic generally shows rapid spread of disease resulting in high mortality rate as well as severe economic damages. Because Korea is contiguous with China and southeast Asia where HPAI have occurred frequently, there is a high risk for HPAI outbreak. A prompt treatment against epidemics is most important for prevention of disease spread. The spread of HPAI should be considered by both direct and indirect contact as well as various spread factors including airborne spread. There are high risk of rapid propagation of HPAI flowing through the air because of collective farms mostly in Korea. Field experiments for the mechanism of disease spread have limitations such as unstable weather condition and difficulties in maintaining experimental conditions. In this study, therefore, computational fluid dynamics which has been actively used for mass transfer modeling were adapted. Korea has complex terrains and many livestock farms are located in the mountain regions. GIS numerical map was used to estimate spreads of virus attached aerosol by means of designing three dimensional complicated geometry including farm location, road network, related facilities. This can be used as back data in order to take preventive measures against HPAI occurrence and spread.
송윤경 ( Yun Kyung Song ),정병열 ( Byeong Yeal Jung ),최정수 ( Jeong Soo Choi ),문운경 ( Oun Kyung Moon ),이수형 ( Soo Hyung Lee ),정덕화 ( Duck Hwa Chung ),박선일 ( Son Il PAK ),박최규 ( Choi Kyu Park ) 한국수의공중보건학회 2010 예방수의학회지 Vol.34 No.3
Aflatoxins produced by Aspergillus spp. are recognized as a major concern in animal and human health. In pigs, ingestion of aflatoxin-contaminated feeds causes immunosuppression, hepatotoxicosis and poor feed efficiency. In this study, we screened the contaminated level of aflatoxins in 449 pig feeds from 12 swine farms in Korea. For rapid and efficient screening of aflatoxins in pig feeds we evaluated the feasibility of three commercial ELISA kits for the screening of aflatoxins in pig feeds. Twenty-nine pig feed samples were examined for total aflatoxins using three ELISA kits, simultaneously. From three repetitions of each assay, the average intra-assay precisions and the average inter-assay precisions expressed as coefficient of variation (CV, %) for Veratox(R) Quantitative Aflatoxin test were 6.90 and 12.29, respectively. A statistical comparison of the results between HPLC and ELISAs showed that the correlation coefficient values for Veratox(R) was 0.96. The results demonstrated that we can apply the Veratox(R) Quantitative Aflatoxin test for the detection of aflatoxins in pig feed from the field. The screening of field samples with this ELISA kit showed that 11 out of 265 pig feeds for growers and weaners were contaminated with total aflatoxin levels exceeding 10 ppb, maximum tolerable limits for their compound feeds and the aflatoxins levels of remaining 184 pig feeds for other age groups of pigs were confirmed as below 20 ppb. The results from the screening indicated overall low levels of aflatoxins contamination in pig feeds.
양돈장의 소모성질병 확산 분석을 위한 현장 모니터링 및 공기유동학적 분석
서일환,이인복,문운경,권경석,Seo, Il-Hwan,Lee, In-Bok,Moon, Oun-Kyung,Kwon, Kyeong-Seok 한국농공학회 2014 한국농공학회논문집 Vol.56 No.1
Pig chronic wasting disease, including porcine reproductive and respiratory syndrome (PRRS) and postweaning multisystemic wasting syndrome (PMWS), have made a continuous economic damage in pig farms. Airborne spread of livestock viruses are an important spread factor which is difficult to analyze due to invisible airflow and limitation of measurement. The objective of this study is to analyze airborne disease spread between buildings in the experimental pig farm by means of field experiment and computational fluid dynamics (CFD). The field experiments were conducted to capture airborne virus using air sampler and teflon filter along multi points in the experimental pig farm. The samples were tested in terms of virus detection resulting in positive reaction for PRRS and PCV-2 viruses, which can be a firm evidence of airborne virus spread. The CFD simulation model was developed by considering complex topography, wind conditions, building arrangement, and ventilation systems and was used to analyze airborne virus spread according to different wind conditions. The CFD computed result showed a possibility of airborne virus spread via livestock aerosol from infected pig house to neighboring pig houses according to wind directions. The CFD simulation technique is expected to provide significant data for estimating and making a counterplan against airborne disease spread.
In situ hybridization 조직화학법을 이용한 오제스키병 바이러스 동정
김순복,서정향,문운경,Kim, Soon-bok,Sur, Jung-hang,Moon, Oun-kyung 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.2
The purpose of this study was to establish a rapid diagnostic method detecting Aujeszky's disease virus (ADV) DNA in the cultured cell monolayers (PK-15) and tissue sections of ADV(NYJ-1-87)-infected rats and pigs by in situ hybridization(ISH). Detection of specific ADV-DNA in infected cells was conducted by radiolabeled ISH method using $^{32}P-labeled $ DNA probe (BamH1 7 fragment) which contains a 6.3 Kb ADV-DNA insert. Where ADV-DNA was detected by radiolabeled ISH, the deposition of black photographic grains occurred in the nuclei and the cytoplasms of ADV-infected cells. Positive hybridization signal was often observed in the spinal trigerminal nucleus of the pons, the nucleus of the trigerminal ganglion neuron and the epithelial cells of tonsillar crypts. The results suggested that ISH is considered as a highly sensitive and reliable tool for confirmative diagnosis of this viral disease.
중합효소연쇄반응을 이용한 돼지 증식성 장염 진단기법 확립
임숙경,이희수,우승룡,윤순식,문운경,이유영,고홍범,Lym, Suk-kyung,Lee, Hee-soo,Woo, Sung-ryong,Yoon, Soon-seek,Moon, Oun-kyong,Lee, Yoo-young,Koh, Hong-bum 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.1
Porcine Proliferative Enteropathy(PPE) is an infectious enteric disease and a major cause of economic loss in swine industry due to weight loss, poor growth and sudden death in growing and finishing pigs at 6 to 20 weeks of age. PPE has been diagnosed by clinical signs, syndrom and lesions in the intestine in Korea. However, the diagnostic method had several problems in the detection of infected or carrier pigs. Therefore, in this study, we established the polymerase chain reaction(PCR) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect 1ng of genomic DNA as a template. Identity of the PCR products was confirmed by comparison of pattern of restriction endonuclease analysis with restriction enzyme Hae III and Pst I. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific diagnostic tool for PPE.
전산유체역학 기법을 이용한 돈사 내 습식 공기 정화기의 적정 위치 설계
권경석,이인복,황현섭,홍세운,서일환,최지선,송상현,문운경,Kwon, Kyeong-Seok,Lee, In-Bok,Hwang, Hyun-Seob,Bitog, Jessie.P.,Hong, Se-Woon,Seo, Il-Hwan,Choi, Ji-Sun,Song, Sang-Hyeon,Moon, Oun-Kyung 한국농공학회 2010 한국농공학회논문집 Vol.52 No.3
In South Korea, as the living standard has been getting higher, meat consumption is steadily increasing. To meet the country's demand, livestock houses become larger and wider with increased raising density. In larger livestock houses, pollutants such as flake of pig skin, excrement, odor, various dusts and noxious gas like ammonia are excessively accumulated inside the facility. These will cause weak immunity for the pigs, diminution of productivity and degeneration of working condition. These problems can be solved through the ventilation performance of the facility. In the winter time, ventilation must be controlled to minimum to maintain a suitable thermal condition. However, this affects the other internal environmental condition because of the minimum ventilation. The installation of "wet air cleaner" especially in the winter time can be an alternative solution. For efficient application of this machine, there is a need to understand the existing ventilation condition and analyze the interaction of existing ventilation system with the wet air cleaner considering its appropriate location. In this study, the existing ventilation system as well as the internal environmental condition negatively inside the facility with the wet air cleaner has been studied using CFD technology. The CFD simulation model was validated from the study conducted by Seo et al. (2008). Results show that the elimination rate of ammonia was 39.4 % and stability could be improved to 35.1 % (Comparing case 5 to 1 where wet air cleaner machine was not used). It can therefore be concluded that case 5 shows the optimum location of a wet air cleaner in the livestock house.
서일환 ( Il Hwan Seo ),이인복 ( In Bok Lee ),황현섭 ( Hyun Seob Hwang ),배연정 ( Yeon Jeong Bae ),배승종 ( Seung Jong Bae ),문운경 ( Oun Kyung Moon ) 한국농촌계획학회 2013 농촌계획 Vol.19 No.4
Most of livestock houses are concentrated in certain area with mass rearing system resulting in rapid spread of infectious diseases such as HPAI (highly pathogenic avian influenza). The livestock-related vehicles which frequently travel between farms could be a major factor for disease spread by means of transmission of airborne aerosol including pathogens. This study was focused on the quantitative measurement of aerosol concentration by field experiment while vehicles were passing through the road. The TSP (total suspended particle) and PM10 (particle matter) were measured using air sampler with teflon filter installed downward the road with consideration of weather forecast and the direction of road. And aerosol spectrometer and video recorders were also used to measure the real-time distribution of aerosol concentration by its size. The results showed that PM2.5 was not considerable for transmission of airborne aerosol from the livestock-related vehicle. The mass generated from the road during the vehicle movement was measured and calculated to 241.4 μg/㎥ by means of the difference between TSP and PM2.5. The dispersion distance was predicted by 79.6 m from the trend curve.