http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
홍화씨 추출물이 MC3T3-E1 조골세포의 증식과 분화에 미치는 영향
이승용 ( Sung Yong Lee ),김수관 ( Su Gwan Kim ),이슬아 ( Seul Ah Lee ),박민경 ( Min Gyeong Park ),박선영 ( Sun Young Park ),오지수 ( Ji Su Oh ),김진수 ( Jin Soo Kim ),이동설 ( Dong Seol Lee ),문소라 ( So Ra Moon ),모신엽 ( Shin Ye 조선대학교 치의학연구원 2013 Oral Biology Research (Oral Biol Res) Vol.37 No.2
Purpose: The main aim of this study was to determine the effect of Carthamus tinctorius seed extract in the proliferation and differentiation of MC3T3-E1 osteoblastic cell line. Materials and Methods: To determine the characteristic alteration of MC3T3-E1 cells by C. tinctorius, it was examined by cell culture, MTT assay, Von Kossa staining, reverse transcriptase-polymerase chain reaction and quantitative real-time polymerase chain reaction analysis in MC3T3-E1 cells. Results: The treatment of C. tinctorius did not significantly affect the cell proliferation in MC3T3-E1 cells. C. tinctorius significantly accelerated the mineralization as compared with the control culture in the differentiation of MC3T3-E1 cells. C. tinctorius significantly increased the expression of alkaline phosphatase mRNA as compared with the control in the differentiation of MC3T3-E1 cells. C. tinctorius significantly accelerated the decrease of osteonectin mRNA expression as compared with the control in a time-dependent manner in the differentiation of MC3T3-E1 cells. Carthamus tinctorius significantly increased the expression of osteocalcin mRNA as compared with the control in the differentiation of MC3T3-E1 cells. Conclusion: These results suggest that the C. tinctorius facilitate the osteoblast differentiation and mineralization in the differentiation of MC3T3-E1 osteoblastic cells, and therefore, that it may have potential properties for development and its clinical application of bone regeneration materials.