구강편평세포암종 이식 누드마우스에서의 혈액 점도 변화

명훈(Hoon Myoung),이종호(Jong-Ho Lee),정필훈(Pill-Hoon Choung),김명진(Myung-Jin Kim) 대한구강악안면외과학회 2004 대한구강악안면외과학회지 Vol.30 No.2

It is well known that malignant tumor have hypoxic cell fraction, which is radio resistant and is one of the most important cause of local recurrence after radiotherapy. One of the causes of hypoxia in tumor is blood flow decrease due to increase in blood flow resistance and one of the causes of increased blood flow resistance could be attributed to the increase in blood viscosity. For the evaluation of the change of blood viscosity in oral cancer, experiments were carried out to test the change of blood viscosity among the normal control and xenografted oral cancer nude mice. Relative viscosity measured against distilled water was 3.30±0.14 for normal control, and 3.67±0.62 for tumor bearing mice at the first time of blood sampling in experimental period (100 mm3 〈tumor volume〈200 mm3). There was no statistically significant difference between the control group and experimental group (p〉0.05). However, as the tumor grew, significant difference of blood viscosity was detected at the third time of blood sampling (control group:3.37±0.59, and experimental group: 4.31±0.41 300 mm3 〈tumor volume, p〈0.05). Increase in blood viscosity was confirmed with this experimental study and it can be postulated that modification blood viscosity might contribute to decrease of hypoxia fraction in oral cancer, thus improve the effect of radiotherapy.

유동적 결합성을 가지는 다중프로세서의 구조와 성능분석

선우 명훈(Sunwoo Myung Hoon) 한국정보과학회 1994 정보과학회논문지 Vol.21 No.12

일반적으로 다중프로세서에서 프로세서들간의 데이타 공유를 위하여 두가지 방식이 주로 쓰이는데 이는 메시지 전달 방식 및 공유 기억소자 방식이다. 그러나 전자는 통신부담이 결점이며 후자는 공유기억소자 분쟁의 단점이 있다. 또한 비효율적인 데이타 입출력 구조도 성능향상에 있어 제한사항이다. 본 논문에서는 위의 단점들을 경감시킬 수 있는 유동적인 연결구조를 갖는 새로운 다중프로세서 (Flexibly Coupled Multiprocessors)를 제안한다. 이 다중프로세서의 구현을 위하여 가변길이 기억소자 (Variable Address Space Memory)를 제안하는데 이는 이웃한 기억소자 모듈들이 필요에 따라 재분할이 가능한 버스구조에 의하여 하나의 기억소자 모듈이 될 수 있다. 따라서 이 가변길이 기억소자 모듈들은 공유 기억소자 또는 비공유 기억소자로 사용된다. 제안하는 다중프로세서의 정량적 모델을 정립, 분석하였고, 상용의 하이퍼큐브 구조 다중프로세서인 Inter's Personal SuperComputer (iPSC)를 이용하여 시뮬레이션 하였다. 성능평가를 위하여 병렬처리 알고리즘들을 개발하여 실지로 iPSC상에서 구현하였으며 제안하는 다중프로세서의 정량적 모델을 이용, 시뮬레이션을 통한 비교 분석을 수행하였다. 실험결과 제안하는 다중프로세서가 iPSC보다 성능에서 월등함을 입증하였다. Two hardware mechanisms are mainly used for data sharing among processors in existing multiprocessors: message passing in loosely coupled multiprocessors and shared memory in tightly coupled multiprocessors. The former has communication overhead and the latter has shared memory contention. Moreover, inefficient data input and output (I/O) schemes are also limitations on performance. This paper proposes a new architecture, called the Flexibly (Tightly/Loosely) Coupled Multiprocessors (FCM), to alleviate these disadvantages. A variable address space memory scheme, in which a set of adjacent memory modules can be merged by a dynamically partitionable bus, is proposed to realize FCM. Computational models of FCM are established, quantitatively analyzed and simulated on the iPSC (Intel's Personal SuperComputer), a hypercube multiprocessor. Parallel algorithms for region labeling and median filtering are implemented on the iPSC and simulated on FCM. The performance of FCM shows remarkable improvement over that of the iPSC.

Genistein을 투여한 햄스터 협낭 구강암 모델에서의 Vascular Endothelial Growth Factor (VEGF) 발현 변화에 대한 면역조직화학적 연구

명훈(Hoon Myoung),김영연(Young-Yun Kim),최상묵(Sang-Mook Choi),정종평(Chong-Pyoung Chung),김명진(Myung-Jin Kim) 대한구강악안면외과학회 2002 대한구강악안면외과학회지 Vol.28 No.2

Genistein that is a component of soy has been reported to have a protective effect on the carcinogenesis of various tumors and to inhibit the growth of a wide variety of tumor cell in vitro. Angiogenesis is an essential process for the carcinogenesis, growth, invasion and metastasis of cancer and genistein has been suggested to act as natural anti-angiogenic agent. The purpose of this study was to evaluate the effects of genistein on the vascular endothelial growth factor (VEGF) expression in hamster buccal pouch oral carcinogenesis model induced by 9, 10-dimethyl 1,2-benzanthracene (DMBA). Experimental group that were supplied with 0.1mg/day genistein were sacrificed by time schedules and routinely processed for immunohistochemical examination of VEGF. In genistein treated group, carcinogenesis was retarded with respect to the acanthosis, hyperkeratosis, and epithelial dysplasia. Immunohistochemical study showed that the VEGF protein of genistein group was less expressed than that of the control group. (p<0.05) Thus, it is postulated that genistein has chemopreventive effect on the oral carcinogenesis, and this chemopreventive effect, at least partly, is originated from the anti-angiogenic effect of genistein

화학 발광 검사법을 이용한 구강 편평 태선의 진단

명훈(Hoon Myoung),정해석(Hae-Seok Jeong),이현선(Hyun-Sun Lee),홍순민(Soon-Min Hong),김명진(Myung-Jin Kim) 대한구강악안면외과학회 2007 대한구강악안면외과학회지 Vol.33 No.4

Purpose: This study was conducted in order to evaluate how chemiluminescent lighting (ViziLite) could increase the sharpness of margin and contrast to normal mucosa in the diagnosis of oral lichen planus (OLP), the most frequent oral premalignant lesion, compared with direct visual inspection under incandescent light. Methods: 41 consecutive patients, diagnosed to have OLP with visual inspection under incandescent light, were further examined with chemiluminescent light. The degrees of margin sharpness and lesion contrast were ranked on a scale from 1 to 3 for all patients under visual inspection and chemiluminescent light. The presence of additional lesion only detected by chemiluminescent light, complication, and discomfort were checked for each patient. After both screening tests, biopsy specimens were harvested from all patients with scalpels and histopathologic assessments were done. Results: All 41 patients were diagnosed to have OLP by both visual inspection and chemiluminescent light examination. This result was definitively diagnosed by histopathology. Degrees of margin sharpness and lesion contrast were increased by chemiluminescent light compared with visual inspection, but only the difference of lesion contrast was statistically significant. In 22.0% of patients, additional lesions were detected and 88.9% of them were diagnosed to have OLP histopathologically. 17.1% of patients noted discomfort and 9.8% of patients showed complications after chemiluminescent test. Conclusion: Chemiluminescent light may not be proper for the screen test of oral cancer or premalignant lesion but showed some possibility for additional diagnostic tool for definitively diagnosed patients in determination of lesion margin and scope

구강편평상피세포암 세포주와 골육종 세포주에서 Taxol?怜? Cisplatin의 항암 효과에 대한 연구

명훈(Hoon Myoung),김명진(Myung Jin Kim) 대한구강악안면외과학회 1999 대한구강악안면외과학회지 Vol.25 No.3

Object Docetaxel (TaxolR), which has been used for breast cancer and ovarian cancer and cisplatin were applied to KB and HOS cell lines. Relative cytotoxicity of each drug to cell lines and its efficiency was evaluated and analyzed for clinical application. Method and Material Groups were defined by drugs and cell lines combination and were evaluated. We cultured cell line under routine environment and determined optimal cell number and drug concentration. Drugs were applied to cell lines and we measured the optical density with ELISA after MTT application. Statistical analysis was made and clinical availability was evaluated. IC50 and assay AUC could be calculated. Clinical availability was evaluated and comparisons of anti-tumor effect of each drug on cell lines were performed. Results 1. Anti-tumor effects of taxol and cisplatin on each cell lines showed time and dose dependent relations with significance statistically. 2. Assay AUC in IC50 was higher than clinically achievable AUC in all groups except cisplatin-KB group. Anti-tumor effect of taxol to KB cell line was higher than that of cisplatin in concentration calculated from the clinically achievable AUC. So, we could suggest that taxol could be also used as a antitumor durg for oral cancer. Further study that is, in vivo study, evaluation of combination therapy with other drug, and method of increasing of AUC would be necessary.

TP 케이블을 이용하는 이더넷 수신기를 위한 디지털 신호 처리부 설계

홍주형,선우명훈,Hong, Ju-Hyung,SunWoo, Myung-Hoon 한국통신학회 2007 韓國通信學會論文誌 Vol.32 No.8a

본 논문에서는 TP 케이블을 이용하여 100Mbps의 전송 속도를 지원하는 100Base-TX Ethernet 수신기의 디지털 신호 처리부를 제안하였다. 제안하는 디지털 신호 처리부는 자동 이득 조절기, 심볼 동기 복원기, 적응 등화기, BLW 보정기로 구성되어 있으며 초기 위상에 상관없이 150m까지 $10^{-12}BER$이하의 성능을 보였다. 제안하는 신호 처리부는 일부 블록을 제외한 모든 부분을 디지털로 구현하였으며 적응 등화기와 BLW 보정기 연동 구조는 기존의 적응 등화기 에러 값을 이용하는 구조에 비하여 MSE가 약 1dB정도의 성능 향상을 가져왔다. 설계한 디지털 신호 처리부는 Verilog-HDL로 구현되었으며 삼성 $0.18{\mu}m$ 라이브러리를 사용하여 합성 결과 동작 속도는 7.01ns 이며 총 게이트 수는 128.528 게이트였다. This paper presents the digital signal processing submodule of a 100Base-TX Ethernet receiver to support 100Mbps at TP cable channel. The proposed submodule consists of programmable gain controller, timing recovery, adaptive equalizer and baseline wander compensator. The measured Bit Error Rate is less than $10^{-12}BER$ when continuously receiving data up to 150m. The proposed signal processing submodule is implemented in digital circuits except for PLL and amplifier. The performance improvement of the proposed equalizer and BLW compensator is measured about 1dB compared with the existing architecture that removes BLW using errors of an adaptive equalizer. The architecture has been modeled using Verilog-HDL and synthesized using samsung $0.18{\mu}m$ cell library. The implemented digital signal processing submodule operates at 142.7 MHz and the total number of gates are about 128,528.

누드마우스에 이종이식된 구강편평상피세포암종에 대한 thalidomide의 항암효과와 혈관형성억제에 관한 연구

김수곤,명훈,김명진,Kim, Su-Gon,Myoung, Hoon,Kim, Myung-Jin 대한구강악안면외과학회 2001 대한구강악안면외과학회지 Vol.27 No.4

Angiogenesis is an essential process for the growth, invasion and metastasis of cancer. However, it is uncertain that antiangiogenic effects can be a major treatment strategy of oral cancer. The aim of this study was to investigate whether thalidomide, which is known to be a potent inhibitor of angiogenesis, have inhibitory effect on the growth and antiangiogenic effects of oral squamous cell carcinoma(OSCC) xenografted in nude mice and whether antiangiogenesis of thalidomide can be included as a major treatment strategy of oral cancer. After human oral squamous cell carcinoma strain KB was subcutaneously implanted in 20 nude mice, the volume of tumor was measured every three days. When the tumor mass reached $75{\sim}100mm^3$, thalidomide(200mg/kg/d) was administered into 10 experimental nude mice and the same volume of distilled water was administered into 10 control nude mice and the tumor volume was measured every three days. The excised tumor masses on the 30th day after administration were frozen and processed for immunohistochemistry using vascular endothelial growth factor(VEGF) and CD31. We evaluated microvessel density and VEGF expression. The results were as follows ; 1. Thalidomide retarded the growth of human OSCC as compared with the control group, but it was not statistically significant. 2. A statistically significant lower microvessel density was observed in the thalidomide-treated group than in the control group(p<0.01) and thalidomide significantly reduced VEGF expression (p<0.01). Thalidomide exhibited significantly antiangiogenic effect, but did not inhibit the growth of human OSCC effectively. Antiangiogenic therapy of thalidomide alone is not likely to be effective in the treatment of human OSCC, but might be regarded as adjuvant chemotherapeutic strategy.

통신 시스템을 위한 고성능 재구성 가능 코프로세서의 설계

정철윤,선우명훈,Jung Chul Yoon,Sunwoo Myung Hoon 대한전자공학회 2005 電子工學會論文誌-SD (Semiconductor and devices) Vol.42 No.6

본 논문은 통신 시스템에서 요구하는 다양한 연산과 고속의 동작을 수행할 수 있는 재구성 가능 코프로세서를 제안하였다. 제안된 재구성 가능 코프로세서는 스크램블링, 인터리빙, 길쌈부호화, 비터비 디코딩, FFT 등과 같은 통신 시스템에 필수적인 연산 동작을 쉽게 구현할 수 있는 특징을 가진다. 제안된 재구성 가능 코프로세서는 VHDL로 설계하여 SEC 0.18$\mu$m 표준셀 라이브러리를 이용해 합성하였으며, 총 35,000 게이트에 3.84ns의 최대 동작 속도를 보였다. 제안된 코프로세서에 대한 성능검증 결과 IEEE 802.11a WLAN 표준에 대해 기존 DSP에 비해서 FFT 연산과 Complex MAC의 경우 약 $33\%$, 비터비 디코딩의 경우 약 $37\%$, 스크램블링 및 길쌈부호화의 경우 약 $48\%\~84\%$의 연산 사이클 감소를 확인하였으며 다양한 통신 알고리즘에 대해 기존 DSP보다 우수한 성능을 나타내었다. This paper proposes a reconfigurable coprocessor for communication systems, which can perform high speed computations and various functions. The proposed reconfigurable coprocessor can easily implement communication operations, such as scrambling, interleaving, convolutional encoding, Viterbi decoding, FFT, etc. The proposed architecture has been modeled by VHDL and synthesized using the SEC 0.18$\mu$m standard cell library. The gate count is about 35,000 gates and the critical path is 3.84ns. The proposed coprocessor can reduced about $33\%$ for FFT operations and complex MAC, $37\%$ for Viterbi operations, and $48\%\~84\%$ for scrambling and convolutional encoding for the IEEE 802.11a WLAN standard compared with existing DSPs. The proposed coprocessor shows Performance improvements compared with existing DSP chips for communication algorithms.

비터비 알고리즘의 효율적인 연산을 위한 DSP 구조 설계

박원흠,선우명훈,오성근,Park Weon heum,Sunwoo Myung hoon,Oh Seong keun 한국통신학회 2005 韓國通信學會論文誌 Vol.30 No.3a

본 논문은 다양한 무선 통신 표준에서 사용되는 비터비 알고리즘을 위한 전용의 DSP 명령어 및 하드웨어 구조를 제안한다. 제안한 구조는 비터비 알고리즘의 Trace Back(TB) 연산 사이클을 효과적으로 줄일 수 있다. 제안된 비터비 전용 명령어와 하드웨어 구조는 비터비 연산의 Add Compare Select(ACS) 연산 과정과 TB 연산 과정의 병렬 처리가 가능하며, 병렬 연산을 지원하기 위해 트랠리스 버터플라이 연산 과정에서 필요한 데이터를 자동으로 생성하는 Offset Calculation Unit(OCU)을 제안한다. 제안된 OCU는 삼성 SEC 0.18μm라이브러리로 로직 합성하여 1,460 게이트 개수를 가지며, 최대 지연 시간은 5.75ns를 나타내었다. 사용된 ACS-TB 병렬 처리 방식은 Eb/No 값이 6dB인 경우 MLSE 등화기 사용 사용되는 일반적인 TB 연산 방식과 비교하여 거의 동일한 BER 성능을 보여 주었으며, 제안한 DSP는 구속장 K=5 일 때 Carmel DSP와 비교하여 11%, TI TMS320c55x와 비교하여 45%의 연산 사이클이 줄일 수 있다. This paper presents specialized DSP instructions and their architecture for the Viterbi algorithm used in various wireless communication standards. The proposed architecture can significantly reduce the Trace Back (TB) latency. The proposed instructions perform the Add Compare Select (ACS) and TB operations in parallel and the architecture has special hardware, called the Offset Calculation Unit (OCU), which automatically calculates data addresses for the trellis butterfly computations. Logic synthesis has been Performed using the Samsung SEC 0.18 μm standard cell library. OCU consists of 1,460 gates and the maximum delay of OCU is about 5.75 ns. The BER performance of the ACS-TB parallel method increases about 0.00022dB at 6dB Eb/No compared with the typical TB method, which is negligible. When the constraint length K is 5, the proposed DSP architecture can reduce the decoding cycles about 17% compared with the Carmel DSP and about 45% compared with 7MS320c15x.

Genistein이 사람 섬유육종 세포주 증식 및 Membrane Type 1-Matrix Metalloproteinase (MT1-MMP) mRNA 발현에 미치는 영향

강진한,명훈,김명진,Kang, Jin-Han,Myoung, Hoon,Kim, Myung-Jin 대한구강악안면외과학회 2001 대한구강악안면외과학회지 Vol.27 No.4

Matrix metalloproteinases have long been viewed as ideal candidates for proteinases that enables tumor cells to permeated basement membrane defenses and invade surrounding tissue. There is growing evidence that the MMPs have an expanded role, as they are important for the creation and maintenance of a microenvironment that facilitates growth and angiogenesis of tumors at primary and metastatic sites. MT-MMPs are not secreted but instead remaining attached to cell surfaces. Although not all of the MT-MMPs are fully characterized, MT-MMPs have important role in localizing and activating secreted MMPs. The MMP genes are transcriptionally responsive to a wide variety of oncogene, growth factors, cytokine, and hormones. Currently, a number of MMP inhibitors are being developed and some have reached clinical trials as anti-metastatic or anti-cancer therapies. MT1-MMP is involved in the activation of proMMP-2. MT1-MMP is significant not only as a tumor marker but as a new target for chemotherapy against cancer. The purpose of this study was to evaluate the effects of protein kinase C inhibitor(genistein) on the proliferation of HT1080 and expression of MT1-MMP mRNA. Human fibrosarcoma cell line HT1080 was cultured and divided 2 groups. The experimental group was treated with $100{\mu}M$ genistein and incubated 12h, 24h for $[3^H]-thymidine$ uptake assay and northern hybridization individually. And the control group was treated with same amount of PBS for the above procedures. $[3^H]-thymidine$ incorporation was measured with ${\beta}$ ray detector. And RT-PCR and northern blotting for MT1-MMP mRNA was performed. The results were as follows 1. $[3^H]-thymidine$ uptake was reduced in experimental group with statistical significance. 2. MT1-MMP mRNA expression was significantly reduced in experimental group. These results showed that protein kinase C inhibitor (genistein) inhibited proliferation of HT1080 and almost completely blocked transcription of MT1-MMP mRNA. So, it is possible to use the protein kinase inhibitor (genistein) as anti-metastatic and anti-proliferative agent.