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오미자의 모유두세포 증식 활성성분과 반응표면분석을 이용한 추출조건의 최적화
조현대 ( Hyun Dae Cho ),정지연 ( Jiyeon Jeong ),류화선 ( Hwa Sun Ryu ),이정노 ( Jungno Lee ),박성민 ( Sung-min Park ) 대한화장품학회 2020 대한화장품학회지 Vol.46 No.4
본 연구에서는 오미자 열매로부터 모유두세포 증식에 활성을 나타내는 gomisin N을 정제하고 gomisin N의 함량이 높은 추출물을 얻기 위한 최적의 추출조건을 확인하였다. 추출물 및 분획물에 대하여 모유두세포증식 활성 실험을 진행한 결과, n-hexane 분획물 1 μg/mL 처리군에서 약 29%의 증식 활성을 나타내었다. n-Hexane 분획물의 활성성분을 규명하기 위하여 column chromatography를 진행하였으며 2 개의 화합물 gomisin N (1)과 schizandrin (2)를 분리 동정하였다. 분리된 화합물에 대한 모유두세포 증식 활성 실험 결과, gomisin N에서 최대 20%이상의 증식 활성을 나타내었다. 따라서 오미자 열매로부터 최대치의 gomisin N을 얻을 수 있는 최적추출조건을 반응표면분석(RSM)을 사용하여 확인하였다. 최적추출조건을 설정하기 위해 에탄올의 비율, 추출시간, 추출온도를 독립변수로 하여 실험한 결과, 0.95 이상의 결정계수와 0.05 이하의 p-value를 나타내어 모델의 적합성이 확인 되었다. Gomisin N의 최대함량을 위한 최적추출조건은 에탄올비율 83.8%, 추출온도 80 ℃, 추출시간 8.7 h으로 확인되었고, 최적추출조건에서 gomisin N의 함량은 378,300 ppm으로 예측되었다. 최적추출조건에 대한 실제 검증에서 평균 376,884 ppm으로 예측된 값과 유사한 결과를 확인 할 수 있었다. In the present study, we have refined gomisin N, which represents activity in the proliferation of dermal papilla cells (HFDPCs) from the fruit of Schisandra chinensis (S. chinensis), and have identified optimal extraction conditions for obtaining extracts with high content of gomisin N. The activity of the extracts and fractions was evaluated, and the results indicated approximately 29% proliferation activity in the group treated with 1 μg/mL of n-hexane fraction. Column chromatography was used to assess the active ingredient in the n-hexane fraction, and two compounds, namely gomisin N(1) and schisandrin(2), were isolated and identified. When the HFDPCs proliferation activity was tested for the isolated compounds, gomisin N exhibited ≥ 20% proliferation activity. Thus, via response surface methodology (RSM), the optimum extraction conditions to obtain the maximum level of gomisin N from the fruit of S. chinensis were determined, where ethanol proportion, extraction time, and extraction temperature were used as the independent variables. The results revealed coefficient of determination ≥ 0.95 and p-value ≤ 0.05, which confirmed the fit of the model. The optimum extraction conditions to achieve the maximum content of gomisin N were as follows: ethanol proportion 83.8%, extraction temperature 80 ℃, and extraction time 8.7 h. The content of gomisin N using these conditions was predicted as 378,300 ppm, and a mean value close to the predicted value (376,884 ppm) was obtained while validating the aforementioned conditions.
Mesenchymal stem cell therapy of systemic lupus erythematosus
표민지, 이홍경, 박미정, 류화선, 김지성, 박윤수, 김주영, 김용국, 홍진태, 김영수, 한상배 충북대학교 약품자원개발연구소 2013 약학논문집 Vol.28 No.-
Systemic lupus erythematosus (SLE) is a sutoimmune disease and the pathogenesis involves the emergence of autoreactive T and B lymphocytes, production of autoantibodies, formation and deposition of immune complexes in various tissues leading to inflammation in many organs. Recently, growing evidence suggests that mesenchymal stem cells can offer an effective therapy for the SLE patients, resulting in disease clinical remission and improvement of organ dysfunction. In this review, we provide a brief overview of current status of MSC therapy for the treatment of SLE and underlying mechanisms.
Membrance receptors of immunostimulating polysaccharides
김형숙, 김연진, 이홍경, 류화선, 김지성, 신보람, 홍진태, 김영수, 한상배 충북대학교 약품자원개발연구소 2011 약학논문집 Vol.26 No.-
β-glucan glucose polymer found in cell wall of plant, bacteria and fungi, exerts potent effects on immune - stimulation antitumor and antimicrobial activity. For this reason, β-glucan has been used clinically in countries. Mcrophages, dendritic cells and neutrophils express several receptors capable of recognizing β-glucan. Complement receptor 3 (CR3), Lactosylceramide(LacCer), Scavenger receptor (SR), Toll-like receptor (TLR) and dectin-1 have been identified on β-glucan receptors. β-glucan binds to receptor and mediate a series of signal pathways leading to immunological activation. However, a different reaction depending on the glucan structure that careful choice of β-glucan. In this review, we summarise the variety of receptors for β-glucan and will discuss the immune responses.