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RISS 인기검색어
- 검색키워드
- 저자 : 로렌스코플랜드(Lawrence O. Copeland) (검색결과 2 건)
- 무료
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Effect of cold-acclimation on freezing tolerance in canola (Brassica napus L.)
Moon Tae Song(宋文台),로렌스코플랜드(Lawrence O. Copeland) 한국육종학회 1995 한국육종학회지 Vol.27 No.2
Of the many factors influencing winter survival of plants, freezing tolerance is one of the most important. This study was conducted to determine the relationship between in vitro freezing tolerance and winter survival of canola (Brassica napusL.). Six winter cultivars and one spring cultivar that had previously exhibited a broad range of winter survival were examined both in a controlled environment chamber and under field conditions. Freezing tests showed that cold acclimation increased the freezing tolerance of both winter and spring cultivars, and that the differences in freezing tolerance among cultivars were due to their ability to acclimate. Freezing tolerance measured by electroleakage tests was highly correlated with winter survival. This suggests that the electroleakage test can be used to predict the comparative winter survival of canola cultivars and tools for the selection of winter hardy cultivars in plant breeding.
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Expression of two-cold regulated genes of canola responding to cold acclimation
Moon Tae Song(宋文台),로렌스코플랜드(Lawrence O. Copeland) 한국육종학회 1995 한국육종학회지 Vol.27 No.1
Cold acclimation increases freezing tolerance and the changes of gene expression may involve in the enhancement of freezing tolerance during cold acclimation. This study was conducted to determine the responses of two cold-regulated genes in canola (Brassica napus L.), BN28 and BN115, to cold acclimation and find out correlation between increasing of freezing tolerance and the expression of the genes. Six winter cultivars and one spring cultivar that had previously exhibited a broad range of winter survival were examined in a controlled environment chamber. Southern blot analysis revealed that the seven cultivars examined had the same copy number of BN28 and BN115. Northern blot analysis indicated that these genes were induced within one day by cold temperature and turned off immediately by deacclimation. Though time-course increase of transcript in one of the genes was highly correlated with the time-course increase of freezing tolerance, no correlation was found at the cultivar lever at specific time of measurement. However, this does not rule out the possible role of cold-regulated genes in freezing tolerance of canola.
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