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RISS 인기검색어
- 검색키워드
- 저자 : 노그라도캐시린 ( Kathyleen Nogrado ) (검색결과 3 건)
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김제, 익산, 장수 지역의 토양 시료에서 테트라사이클린 내성 박테리아 다중약물 내성발생 및 RPP-tet 유전자검출
노그라도캐시린 ( Kathyleen Nogrado ),이지훈 ( Ji-hoon Lee ) 한국환경농학회 2020 한국환경농학회 학술대회집 Vol.2020 No.-
Multi-drug resistance is another urgent concern raised with antibiotic resistance because it narrows the treatment option, extends ailment which might lead to death if get worsen. Incidentally, antibiotics consumed in large amounts by the agricultural sector has found its way to the environment like soil. This in turn affects functions and causes selective pressure to antibiotic resistance among microbial soil residents. This study determined multi-drug resistance by disk diffusion testing and detected four ribosomal protection (RPP)-tet genes by PCR among tetracycline-resistant bacterial isolates obtained from soils in three agricultural areas. A total of 110 tetracycline-resistant bacterial isolates were obtained and tested. Using disk diffusion, it was determined that 17/110 bacterial isolates were multi-drug resistant (resistant to erythromycin, streptomycin, and tetracycline) and the majority was isolated from Gimje. Disk diffusion test also revealed different pattern of high resistance to individual antimicrobial: Gimje to tetracycline, Iksan to streptomycin, and Jangsu to erythromycin. This pattern could be reflective of which antibiotic is consumed in high amount in that area. On the other hand, PCR testing revealed that all four RPF-tet genes were detected m the culturable bacteria except tetO. PCR testing also revealed that a very small percentage (1.82%) carries 3 RPP tet genes, a slightly higher percentage (12.73%) carries two RPP-tet genes, and most bacterial isolates (31.82%) carries only one BPP-tet gene. The results or the two tests revealed that there were bacterial isolates that were phenotypically resistant but did not carry any of the four EPP-tet genes and also there were bacterial isolates phenotypically susceptible to tetracycline but carries one of the four RPP- tet genes. The former is explained by the presence of other tetracycline resistance gene determinant while the latter indicates that risk is not eliminated as the gene could still be transferred and expressed by other bacterial host. In 2017,a report on the rate of consumption of antibiotics in South Korea showed that tetracycline antibiotics have not been used since 2009; the results of this study could give information on the effect of the intervention applied concerning antibiotic resistance and agricultural health status in terms of the presence of antibiotic-resistant bacteria and antibiotic resistance genes.
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살코스키 시약과 Indole-3-acetic Acid의 반응 중 트립토판의 간섭
이슬 ( Seul Lee ),어나미까커날 ( Anamika Khanal ),여준구 ( Junkoo Yeo ),노그라도캐시린 ( Kathyleen Nogrado ),조아현 ( Ahyeon Cho ),송윤진 ( Yoonjin Song ),권미지 ( Miji Kwon ),이지훈 ( Ji-hoon Lee ) 한국환경농학회 2018 한국환경농학회 학술대회집 Vol.2018 No.-
Indole-3-acetic acid (IAA) is the most common auxin hormone produced in the plant and is very important because it regulates various aspects of plant growth and development. Tryptophan is the main precursor for IAA while IAA can be synthesized by two pathways i.e., tryptophan-dependent pathway and tryptophan-independent pathway. There have been many studies using Salkowski regent to measure the IAA production by bacteria. In this study, we have found that there was the interference of tryptophan on the colorimetric estimations of indole-3-acetic acid (IAA) by using Salkowski reagent (35% HClO4 and 10 mM FeCl3), which developed a pink color complex of tris-(indole-3-acetate) iron(III) with IAA. Moreover, we detected the effect of different concentration of tryptophan (100, 200, and 300 μM) on different concentration of IAA (100, 200, and 300 μM). The absorbance on IAA for the tested concentrations was found to be increased with the increasing concentration of tryptophan. It seems that both the IAA and tryptophan show the maximum absorbance at the similar wavelengths. It was also observed that the difference between the predicted and measured concentrations of IAA became larger as the predicted IAA concentration decreased. From this study, it is expected that tryptophan would interfere with accurate measurements of IAA, and therefore care should be taken for the measurement of IAA generated by the precursor, tryptophan.
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실시간 중합효소연쇄반응을 이용하여 논 토양 내 질소순환 관련 기능 유전자의 평가
어나미까커날 ( Anamika Khanal ),이슬 ( Seul Lee ),여준구 ( Junkoo Yeo ),노그라도캐시린 ( Kathyleen Nogrado ),조아현 ( Ahyeon Cho ),송윤진 ( Yoonjin Song ),권미지 ( Miji Kwon ),이지훈 ( Ji-hoon Lee ) 한국환경농학회 2018 한국환경농학회 학술대회집 Vol.2018 No.-
The nitrogen cycle and the microbes responsible for mediating this process have an important role in natural ecosystems, terrestrial habitats and also have a major impact on climate change. In this study, we use molecular techniques such as conventional PCR, clone library construction, sequencing, phylogenetic analysis and real-time PCR to explore microbial communities in a rice paddy soil by detecting and quantifying some of the key functional genes that are involved in the process of nitrogen cycle i.e., nitrogen fixation (nifH), hydrazine synthase (hzsA), nitrous oxide reductase (nosZ), copper-containing (nirK), cytochrome cd1- containing nitrite reductase (nirS), nitrite oxidoreductase (nxrB), and ammonium monoxygenase (amoA). The sequence assessment from the clone library targeting these different genes i.e., nifH, hzsA, nosZ, nirK, nirS, nxrB, and amoA showed a high diversity and dominance of bacterial community. Besides that, Real-time PCR using SYBR green dye and some old and recently developed primers specific for each individual gene revealed the high abundance of nxrB gene and low abundance of hzsA gene. This finding will be very helpful in exploring and exploiting microorganism in terrestrial habitat.
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