전통누룩 진균류를 이용한 입국의 제조 및 입국곰팡이의 동정

김재호 ( Jae Ho Kim ),권영희 ( Young Hee Kwon ),이애란 ( Ae Ran Lee ),김혜련 ( Hye Ryun Kim ),안병학 ( Byung Hak Ahn ) 한국균학회 2012 韓國菌學會誌 Vol.40 No.4

Various koji were prepared by fungi isolated from traditional nuruk and their quality characteristics were investigated. Acidity and saccharification power of their koji were ranged in 5.0~6.8 and 128sp~241sp. Nine fungi which were showed good quality and sensory evaluation were identified by analysis of their nucleotide sequences with PCR-amplified 18S rDNA internal transcribed spacer-1(ITS-1) and ITS-4 genes. Among them, six strains were identified as Aspergillus oryzae and the other strains were identified as Mycocladus corymbiferus, Rhizopus oryzae, Lichtheimia corymbifera.

면접조사자료와 사망등록자료 간 교육수준 및 직업계층의 신뢰도

김혜련,강영호,Kim, Hye-Ryun,Khang, Young-Ho 대한예방의학회 2005 Journal of Preventive Medicine and Public Health Vol.38 No.4

Objectives : This study was done to evaluate the reliability of education and occupational class between using the health survey and the death certificate data. Methods : The 1998 National Health and Nutrition Examination Survey (NHANES) was conducted on a cross-sectional probability sample of South Korean households, and it contained unique 13-digit personal identification numbers that were linked to the data on mortality from the Korean National Statistical Office. The data from 263 deaths were used to estimate the agreement rates and the Kappa indices of the education and occupational class between using the NHANES data and the death certificate data. Results : The simple and weighted Kappa indices for education were 0.60 (95% CI=0.53-0.68) and 0.73 (95% CI=0.67-0.79) respectively, if the educational level was grouped into five categories: no-formal-education, elementary-school, middle-school, high-school and college or over. The overall agreement rate was 71.9% for these educational groups. The magnitude of reliability, as measured by the overall agreement rates and Kappa indices, tended to increase with a decrease in the educational class. The number of non-educated people with using the death certificate data was smaller than that with using the NHANES data. For the occupational class (manual workers, non-manual workers and others), the Kappa index was 0.40 (95% CI=0.30-0.51), which was relatively lower than that for the educational class. Compared with the NHANES, the number of non-manual workers for the deceased who were aged 30-64 tended to be increased (8 to 12) when using the death certificate data, whereas the number of manual workers tended to be decreased (59 to 41). Conclusions : The socioeconomic inequalities in the mortality rates that were based on the previous unlinked studies in South Korea were not due to a numerator/denominator bias. The mortality rates for the manual workers and the no-education groups might have been underestimated.

와인의 산미와 침전/와인제조를 지탱하는 신기술: 만노단백질

김혜련,Kim, Hye-Ryun 한국식품연구원 2010 食品技術 Vol.23 No.4

우리 나라의 사회경제적 사망률 불평등: 1998년도 국민건강영양조사 자료의 사망추적 결과

김혜련,강영호,Kim, Hye-Ryun,Khang, Young-Ho 대한예방의학회 2006 예방의학회지 Vol.39 No.2

Objectives : This study was conducted to examine the relationships of the several socioeconomic position indicators with the mortality risk in a representative longitudinal study of South Korea. Methods : The 1998 National Health and Nutrition Examination Survey was conducted on a cross-sectional probability sample of South Korean households, and it contained unique 13-digit personal identification numbers that were linked to the data on mortality from the National Statistical Office of Korea. Of 5,607 males and females, 264 died between 1999 and 2003. Cox's regression was used to estimate the relative risks (RR) and their 95% confidence intervals (CI) of mortality. Results: Socioeconomic differences in mortality were observed after adjustments were made for gender and age. Compared with those people having college or higher education, those people without any formal education had a greater mortality risk (RR=2.21, 95% CI=1.12-4.40). The mortality risk among manual workers was significantly greater than that for the non-manual workers (RR=2.73, 95% CI=1.47-5.06). A non-standard employment status was also associated with an increase in mortality: temporary or daily workers had a greater mortality risk than did the full-time workers (RR=3.01, 95% CI=1.50-6.03). The mortality risk for the low occupational class was 3.06 times greater than that of the high and middle occupational classes (95% CI=1.75-5.36). In addition, graded mortality differences according to equivalized monthly household income were found. A reduction of monthly household income by 500 thousand Korean Won (about 400 US dollars) was related with a 20% excess risk of mortality. Self-reported poor living standards were also associated with an increased risk of mortality. Those without health insurance had a 3.63 times greater risk of mortality than the insured (95% CI=1.61-8.19). Conclusions: This study showed the socioeconomic differentials in mortality in a national longitudinal study of South Korea. The existence of socioeconomic mortality inequalities requires increased social discussion on social policies in Korean society. Furthermore, the mechanisms for the socioeconomic inequalities of mortality need to be explored in future studies.

Aspergillus nidulans의 유성분화에 관여하는 nsdC 유전자의 분리 및 분석

김혜련,한동민,Kim, Hye-Ryun,Han, Dong-Min 한국미생물학회 2006 미생물학회지 Vol.42 No.4

사상성 진균인 Aspergillus nidulans에서 유성분화초기단계, 또는 유성분화유도를 위한 세포내 조건 형성과정에 관여할 것으로 예상되는 유전자를 탐색하였다. 선행연구결과를 통해 유성분화를 전혀 하지 못하는 NSD (never in sexual development) 돌연변이주가 분리되어 nsdA, nsdB, nsdC, 그리고 nsdD의 4상 보군으로 동정된 바 있다. 본 연구에서는 이들 유전자 중 nsdC 유전자를 분리하고자 A. nidulans AMAl-Not I Genomic DNA library로 nsdC6 돌연변이균주를 형질전환하여 야생형처럼 유성분화를 할 수 있는 형질전환체를 분리하고 이들로부터 약 10 kb genomic DNA가 삽입된 library DNA를 분리하였다. Genomic priming system (GPS)을 이용하여 nsdC6 돌연변이를 상보하는 유전자의 부분 서열을 확보한 후 전체 DNA 염기서열을 결정하였다. 유전자분석 결과 nsdC는 intron 없이 1,929염기(643개의 아미노산)로 구성된 Open reading frame (ORF)를 가지며, 약 1kb 정도의 비교적 긴 5'-UTR 부위에 2개의 intron을 가지고 있음이 확인되었다. 또한 NsdC polypeptide의 중앙에 $C_2H_2C_2H_2C_2HC$ 형의 zinc finger DNA binding domain과 C 말단 부위에 coiled-coil domain이 존재하였다. nsdC6 돌연변이는ORF의 407 bp와 408 bp사이에 엽기 T가 삽입되어 frameshift가 일어난 것으로 밝혀졌다. 따라서 nsdC6 돌연변이균주는 단지 139개 아미노산만 갖고 있는 결실 단백질이 생산됨을 알 수 있었다. A lot of mutants which cannot initiate sexual development were screened and several loci including nsdA, nsdB, nsdC, and nsdD were identified in homothallic ascomycetes Aspergillus nidulans. The NSD206, which has nsdC6 allele, showed typical phenotype of NSD (Never in sexual development) mutants. The nsdC gene was cloned by transforming NSDP697 ($nsdC^-$, $pryG^-$) with AMA1-NotI genomic library. The transforming library DNA recovered from several transformants showing wild phenotype carried about 10 kb genomic DNA insert. The DNA sequence of nsdC was analysed using GPS (Genome priming system). The nsdC gene has an open reading frame (ORF) of 1,929 bp encoding a putative polypeptide of 643 amino acids. The NsdC carries $C_2H_2C_2H_2C_2HC$ type zinc finger DNA binding domains in the middle of the polypeptide. A coiled-coil domain at its C terminus were also found. In nsdC6 allele, a single T insertion was occurred between 407-408 bp leading to the frameshift mutation and early termination of translation producing the truncated protein which has only 139 amino acids.

한국과 호주의 암환자를 위한 사회 돌봄 서비스 탐색

김혜련,이규선,Kim, Hye-Ryun,Lee, Gyu-Sun 한국호스피스완화의료학회 2012 한국호스피스.완화의료학회지 Vol.15 No.1

목적: 암환자는 질병에 대한 정보나 조언뿐 아니라 정서적, 재정적, 실질적인 지지를 필요로 한다. 본 연구에서는 한국과 호주의 암환자를 위한 사회 돌봄 서비스 제공에 대한 기회와 장애를 살펴보고자 한다. 방법: 한국과 호주의 사회복지사들을 대상으로 이메일을 통해 설문하였으며, Braun과 Clarke의 주제분석방법을 통해 분석하였다. 결과: 양국 모두에서 암환자를 위한 다양한 사회 돌봄 서비스가 제공되고 있었다. 하지만, 한국의 경우는 사회 돌봄에 대한 인식 수준을 우선 높여야 하며, 호주는 보다 개별화된 사회 돌봄 서비스의 제공이 필요했다. 결론: 본 연구결과는 현 상태를 파악하고 약점을 보완하게 함으로써, 궁극적으로 한국과 호주 양국에서 암환자를 위한 사회 돌봄 서비스 개선에 도움이 될 것이다. Purpose: Cancer patients require emotional, financial and practical support as well as information/advice regarding their illness. This study aims to explore opportunities and barriers for the provision of the social support services in Australia and Korea. Methods: The survey was carried out by an email questionnaire for social workers in Australia and Korea, and collected data were analyzed using a thematic analysis by Braun and Clarke. Results: In Australia and Korea, various types of social support were available for cancer patients. However, social support for cancer patients should be better understood first in Korea, and more personalized support is needed in Australia. Conclusion: These findings will ultimately help to improve social support services for cancer patients in Korea an Australia, through grasping the current state and making up for the weak points.

만성질환 유병과 주관적 건강수준의 사회계층별 차이와 건강행태의 영향 - 서울시민을 대상으로-

김혜련 ( Hye Ryun Kim ) 한국보건사회연구원 2005 保健社會硏究 Vol.25 No.2

본 연구의 목적은 우리나라 인구의 1/4이 거주하는 서울시에서 건강수준의 불평등과 관련된 지표를 이용하여 사회계층간 건강수준의 차이를 대표성 높은 서울시민보건지표조사 자료를 이용하여 실증적으로 분석하고, 유병수준과 관련된 사회경제적 요인과 건강행태 요인들의 영향을 분석하고자 하였다. 연구자료는 2001년 서울시민보건지표조사 자료로서 2,500개 조사구에서 조사된 25~64세 인구 16,180명을 대상으로 하였다. 통계분석은 다변량 로지스틱 회귀분석을 이용하였다. 건강수준으로는 만성질환 유무와 주관적 건강수준의 두가지 유병수준 지표를 선정하였으며, 사회계층변수로는 교육수준, 가구 월소득수준, 직업유형을, 건행태요인으로는 흡연, 음주, 비만, 운동, 아침식사 결식여부, 수면적절성 등의 6가지 변수를 선정하였다. 연구 결과 남녀 모두 낮은 교육수준, 낮은 소득수준, 낮은 직업계층의 집단에서 만성질환 유병의 위험도와 주관적 건강수준이 나쁠 위험도가 뚜렷하게 증가하였다. 남녀 모두 사회계층 변수 중 교육수준이 만성질환과 주관적 건강수준에 미치는 영향이 컸다. 사회계층 요인과 건강행태 요인이 모두 유병수준에 영향을 미치는 것으로 나타났으나 사회계층 요인의 영향이 더욱 컸다. 주관적 건강상태는 만성질환에서 보다 사회계층에 따른 불평등도가 더욱 컸으며, 남자가 여자에 비하여 사회계층에 따른 주관적 건강상태의 불평등도가 심하였다. 결론적으로 우리나라 서울시 인구집단에서 사회계층간 만성질환과 주관적 건강수준에서 상당한 불평등이 존재하는 것으로 파악되었다. This study aims to investigate socioeconomic differences in morbidity in Seoul Metropolitan. The study consists of a representative sample of 16,180 adult men and women aged 20~64 who participated in the 2001 Seoul Citizens` Health Interview Survey. This population-based crosssectional survey data was used to investigate the effects of education, income and occupation on the prevalence of self-reported chronic illness from all causes and self-rated health. To estimate the odds ratios and 95% confidence intervals of self-reported chronic illness and self-rated health a multiple logistic analysis was conducted. For both men and women, the study shows that socioeconomic position(SEP) is strongly related with risk of self-reported chronic illness and self-rated health at all levels of the SEP hierarchy, that is, lower education and income led to a significant increase in morbidity. After controlling for age and 6 health behavioral risk factors(duration of smoking, alcohol drinking, relative body weight, physical activity, skipping breakfast and sleeping pattern), the odds ratio of self-reported chronic illness for men was 1.92 (95% confidence interval [CI], 1.49- 2.47), for women 1.89 (95% CI, 1.54-2.32) among those with the lowesteducated group compared to the highest-educated group. When household income was considered, after controlling for age and 6 behavioral risk factors, the odds ratio of chronic diseases for men was 1.12 (95% CI 0.93-1.35), and for women 1.62 (95% CI 1.39-1.89) among those in the lowest-income group compared to the highest-income group. The odds ratio of self-rated health after controlling for age and 6 behavioral risk factors was 2.41 (95% CI, 1.78-3.25) for men, 2.05 (95% CI, 1.63-2.58) for women among those with the lowest-educated group compared to the highest-educated group. When household income was considered, after controlling for age and 6 behavioral risk factors, the odds ratio of self-rated health for men was 1.63 (95% CI 1.25-2.13), and 1.32 (95% CI, 1.09-1.58) for women among those in the lowest-income group compared to the highest-income group. This study finds that there exist large socioeconomic inequalities in morbidity in Seoul Metropolitan area. To be more specific, socioeconomic differences for men are larger in self-rated health, but smaller in chronic illness than women. Also, the effect of education is stronger than that of income for both men and women. The study results are similar to previous studies on social inequalities in health status for Korea and other developed countries.

쌀 가공제품을 위한 다양한 쌀의 품질분석

김혜련(Hye-Ryun Kim),권영희(Young-Hee Kwon),김재호(Jae-Ho Kim),안병학(Byung-Hak Ahn) 한국식품과학회 2011 한국식품과학회지 Vol.43 No.2

쌀 가공제품을 위한 다양한 쌀의 품질분석을 위해 국내산 쌀 19품종과 수입쌀 1종의 일반성분과 형태검정, 이화학적 특성 및 호화특성을 분석하였다. 수분함량은 11.11±0.20-13.28±0.03%였으며 단백질과 지방 함량은 각각 5.04±0.03-7.02±0.10, 0.18±0.01-0.73±0.05%였다. 무기질 함량은 칼슘이 3.56±0.11-6.69±0.08 mg/100 g, 나트륨이 3.39±0.01-17.43±0.04 mg/100 g, 인이 64.12±0.88-102.0±0.36 mg/100 g, 아연이 0.95±0.01-1.75±0.0 mg/100 g, 철이 0.19±0.0-0.69±0.02 mg/100 g, 마그네슘이 9.89±0.47-23.31±0.21 mg/100 g, 칼륨이 47.11±3.49-82.19±1.08 mg/100 g 그리고 망간이 0.47±0.0-1.14±0.01 mg/100 g의 함량을 보였다. 20품종 중 18품종의 크기가 소립이었으며 아밀로즈 함량은 10.3±1.27-19.4±0.15%였고 전분가는 70.8±2.67-80.1±5.09%를 나타내었다. 알칼리 붕괴도는 5-6등급의 상태였고 호응집성의 측정 결과 gel은 63.0±7.0-100±0.0 mm 사이로 흘러 ‘연함’을 나타내 양질의 상태를 보였다. The objective of this study was to analyze proximate composition and physicochemical properties according to different kinds of rice. In total, 20 varieties of rice were used (Domestic-19, Imported-1). The moisture contents ranged from 11.11±0.20 to 3.28±0.03%. The crude protein and crude lipid contents were ranged from 5.04±0.03 to 7.02±0.10% and 0.18±0.01 to 0.73±0.05%, respectively. The mineral contents were Calcium, 3.56±0.11-6.69±0.08 mg/100 g; Sodium, 3.39±0.01-17.43±0.04 mg/100 g; Phosphorus, 64.12±0.88-102.0±0.36 mg/100 g; Zinc, 0.95±0.01-1.75±0.0 mg/100 g; Iron, 0.19±0.0-0.69±0.02 mg/100 g; Magnesium, 9.89±0.47-23.310.21 mg/100 g; Potassium, 47.11±3.49-82.19±1.08 mg/100 g; and Manganese, 0.47±0.0-1.14±0.01 mg/100 g. Eighteen kinds of rice exhibited small kernels. Amylose contents ranged from 10.3±1.27 to 19.4±0.15%, while starch value ranged from 70.8±2.67 to 80.1±5.09%. Alkali digestion value was described as 5-6 grade and gel consistency was shown to be ‘soft’ regardless of the rice kinds.

누룩에서 분리한 효모를 이용한 찹쌀발효주의 이화학적 특성 및 휘발성 향기성분

김혜련(Hye-Ryun Kim),권영희(Young-Hee Kwon),조성진(Sung-Jin Jo),김재호(Jae-Ho Kim),안병학(Byung-Hak Ahn) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.3

효모가 찹쌀 발효주에 미치는 영향을 조사하기 위해 전통 누룩에서 분리한 보유효모 Y18-2(Andong), Y54-3(Chungju), Y90-2(Hapchun), Y90-9(Hapchun) Y272-7(Boryung)과 시판효모 LP(La Parisienne, Saccharomyces cerevisiae)를 대조군으로 효모종류를 달리하여 발효주를 제조하여 이화학적 특성과 휘발성 향기성분을 비교하였다. 17일 발효 후 최종 알코올 함량은 모든 술이 시판효모를 이용한 발효주(13.40±0.70%)보다 높게 나타나 양호한 발효상태를 보였으며 pH는 4.06-4.32, 총산은 0.33-0.44% 수준으로 나타났고 아미노산은 Y90-9를 사용한 발효주가 가장 높게 나타났다. 고형분 함량은 시판효모를 이용한 발효주가 가장 높게 나타났고 착색도는 Y90-9를 사용한 발효주가 가장 낮게 나타나 좋았으며 자외부흡수는 Y90-2를 사용한 발효주가 가장 낮게 나타나 쓴맛이 가장 적을 것으로 추측되고 환원당 함량은 고형분 함량이 가장 높았던 시판효모를 이용한 발효주가 가장 높게 나타나 단맛이 가장 강할 것으로 사료된다. 유기산 중 함량이 가장 많은 succinic acid는 Y90-9를 사용한 발효주에서 가장 높게 나타나 감칠맛 성분이 높은 것을 알 수 있었고 좋지 않은 신맛에 기여하는 lactic acid와 acetic acid는 시판효모를 사용한 발효주에서 가장 높게 나타났다. 발효가 진행되면서 생성되는 대표적인 유리당 성분 maltose와 glucose는 환원당 함량이 가장 높았던 시판효모를 이용한 발효주에서 가장 높게 나타났다. GC와 GC-MSD를 사용한 휘발성 향기성분 분석 결과 Alcohol 10종, Ester 19종, Acid 8종, Aldehyde 1종, 기타 1종으로 39종이 검출되었고 Y18-2를 사용한 발효주에서 가장 많은 향기성분이 확인되었다. 시판효모를 이용한 발효주에서는 ethanol의 면적비율이 가장 높은데 반해 Y18-2와 Y90-9를 사용한 발효주에서는 ester류의 면적비율이 가장 높게 나타났으며 그 중 hexadecanoic acid ethyl ester의 면적비율이 가장 높게 나타나 찹쌀 발효주에서 효모 종류에 따른 향기성분의 많은 차이를 볼 수 있었다. 따라서 발효주 제조 시 원료, 담금 방법 및 발효 조건 등을 최적으로 맞추고 향기성분 생성이 우수한 효모를 사용한다면 색, 맛과 함께 향미가 우수한 찹쌀발효주가 제조되리라고 여겨지며 관련된 많은 연구가 요구된다. In order to investigate the effect of different yeasts (La Parisienne (LP), Y18-2, Y54-3, Y90-2, Y90-9 and Y272-7) from nuruks on the quality of Glutinous rice wines, physicochemical properties and volatile flavor components were evaluated. Glutinous rice wines prepared with different yeasts were analyzed for ethanol, pH, total acid, amino acid, soluble solid, coloring degree, UV absorbance, reducing sugar, organic acid, free sugar and volatile compounds. After fermentation for 17 days, the ethanol contents ranged from 13.40 to 14.50%, while the total acid levels were from 0.33 to 0.44%. The amino acid contents in six samples ranged from 0.13 to 0.18%, while soluble solid contents ranged from 12.1 to 14.7oBx. The glutinous rice wine prepared with LP showed the highest level of coloring degree, soluble solid and reducing sugar among six samples. Organic acid contents of the glutinous rice wine prepared with LP had the highest levels of lactic acid and acetic acid, while the glutinous rice wine prepared with Y90-9 had the highest level of succinic acid. In all glutinous rice wines tested, the most abundant free sugars were glucose followed by maltose. Volatile flavor components in the glutinous rice wines were identified by using GC-MSD. Nineteen esters, ten alcohols, eight acids, one aldehyde and one miscellaneous compound were identified in the glutinous rice wines. Using relative peak area, it was found that other than ethyl alcohol, hexadecanoic acid ethyl ester was the major component, predominantly found in the range of 2.73-10.41%. Phenylethyl alcohol, isoamyl alcohol, ethyl oleate, ethyl linoleate and tetradecanoic acid ethyl ester were some of the major volatile components present through the fermentation, respectively. Overall, it was shown that different yeast strains from nuruks greatly affected chemical and volatile characteristics of the glutinous rice wines.

영국거주 한인학생들의 문화 인식에 대한 조사

김혜련 ( Hye Ryun Kim ) 글로벌영어교육학회 2005 Studies in English education Vol.10 No.1