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NADH oxidase(nox) 유선자의 PCR- RFLP를 이용한 돼지 intestinal spirochetes 국내분리주의 동정
김태중,김명희,나영란,정석찬,이재일,Kim, Tae-Jung,Kim, Myoung-Hee,Na, Young-Ran,Jung, Suk-Chan,Lee, Jae-Il 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.4
In this study, we performed a PCR-RFLP analysis of NADH oxidase gene(nox) for the characterization of porcine intestinal spirochetes isolated from Korea by the comparison with Brachyspira hyodysenteriae and B. pilosicoli reference strains. Eleven strains including four reference strains, B. hyodysenteriae B204, B234, B169, B. pilosicoli P43/6/78 and seven Korean isolates were used. PCR products of 939 bp were amplified using nox-specific primers and digested with two restriction enzymes, Bfm I and Dpn II. In study using Bfm I, both strains showed no difference in fragmented size(197 and 741 bp). When use Dpn II, B. hyodysenteriae showed two bands(209 and 684 bp), however B. pilosicoli showed a single band of 896 bp. Our results indicate that nox-specific PCR-RFLP could be used as a typing method of Brachyspira species and as an epidemiological method for identifying spirochetes isolated from swine.
대장균에서 흉막폐렴균 독소 Apx I과 Apx II의 대량발현
김태중,이봉주,이재일,Kim, Tae-Jung,Lee, Bong-Joo,Lee, Jae-Il 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.2
Actinobacillus pleuropneumoniae is the causative agent of a porcine contagious pleuropneumonia. Among several virulence factors including exotoxin (Apx toxins), LPS, transferrin-binding proteins, OMPs, and some proteases, Apx toxins have been major targets for the protection study. In this study, cloning and expression of A. pleuropneumoniae Apx I and Apx II toxin, which are produced by all highly virulent strains, were performed by Escherichia coli expression system. Genes coding Apx I and II toxin were amplified from the A. pleuropneumoniae serotype 5 genomic DNA using polymerase chain reaction and cloned to a prokaryotic expression vector, pRSET. Expression of the Apx I and Apx II coding sequences in E. coli resulted in the formation of insoluble inclusion bodies purified according to a denaturing purification protocol, which employs the use of guanidium. Recombinant proteins were purified using $Ni^{2+}$-charged resin affinity purification. This expression and purification system made it possible to produce Apx I and Apx II in large amounts for further immunologic studies.
김태중,장은진,김종수,이재일,Kim, Tae-Jung,Jang, Eun-Jin,Kim, Jong-Su,Lee, Jae-Il 대한수의학회 2006 大韓獸醫學會誌 Vol.46 No.1
Iridovirus is an icosahedral cytoplasmic double-stranded DNA virus with a genome size of 170-200kb. Outbreaks of fish iridovirus infection are characterized by their wide geographic distribution and broad host spectrum, especially in water temperatures of $25-27^{\circ}C$ Recently, the causative agent of high mortalities in flounder (Paralichthys olivaceus) was identified as fish iridovirus in Korea. Iridoviral infection repeatedly occurs in the same area for long periods, suggesting the possibility of viral infection in nursery. To examine this, the existence of iridovirus in juvenile flounders was detected by PCR using virus-specific primers. Antibodies induced against this virus were also examined using ELISA. As a result, juvenile fish in nursery were found to be previously infected with iridovirus, suggesting that proper prevention systems are required.
김태중,Kim, Tae-Jung 한국과학기술정보연구원 2004 지식정보인프라 Vol.13 No.-
성과확산과 정책연구는 지금까지의 관점에서 보면 상호 연과성이 없는 듯하나 서로 매우 긴밀한 연관 관계가 있다. 각계의 이용자들이 구축된 인프라를 이용하면서 느끼는 KISTI에 대한 반응을 정책에 반영하여 이용자의 의견을 국가 정책 및 기관의 발전 방향에 반영하기 위한 것으로 한층 성숙된 이용자의 만족도 제고 및 정책개발이 가능할 것으로 기대된다.
데이터베이스의 사용문자(使用文字) 및 용어(用語) 표기법(表記法)
김태중,이창한,Kim, Tae-Jung,Lee, Chang-Han 한국과학기술정보연구원 과학기술정보센터 1988 Journal of Information Science Theory and Practice Vol.19 No.1
지금까지 데이터베이스를 만들 때에 일정한 표기기준(表記基準)이 없어서 정보교환(情報交換)이 불가능하였으며, 검색(檢索)에 있어서도 어려움이 있었다. 이 자료(資料)에서는 검색(檢索)을 위한 데이터베이스를 제작(製作)할 때에 사용하는 기호(記號)와 용어(用語)의 표기법(表記法)을 제시하고 있다. 학술논문(學術論文)을 기술(記述)할 때에 사용되고 있는 기호(記號)와 문자(文字) 가운데 컴퓨터 단말기(端末機)를 통하여 입력(入力) 및 검색(檢索)이 곤란한 경우가 있으므로 이러한 기호(記號)와 문자(文字)를 입력(入力) 검색(檢索)이 가능한 형태(形態)로 표현하는 방법(方法)을 고안했으며, 문교부(文敎部)가 고시한 "한글 맞춤법"과 "외래어 표기법"을 검토하여 2가지 이상으로 표기(表記)가 가능한 부분에 대해 검색효율(檢索效率)이 높아지는 쪽을 택해 일정하게 용어(用語)를 표기(表記)하도록 규정(規定)했다. From the lack of common rule for the description of the characters and terms in bibliographic databases, it was hard to share information with other organizations and to obtain relevant bits of information through online retrieval. In this paper, the authors suggest a rule for the transcription of symbols and letters, that are found in articles. but impossible to input through CRT terminals, into the symbols and letters which is capable of input and retrieval. And, in the 'Hangul orthography' and 'Description rule of the borrowed words' which are officially announced by the Ministry of Education, more than two ways are permited for the expression of terms. In that case, to improve retrieval efficiency and to prevent 'confusion in description, they are regulated.
국내의 개에서 Brachyspira pilosicoli의 분리 및 동정
김태중,이재일,Kim, Tae-Jung,Lee, Jae-Il 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.3
This study was performed to report the outbreaks of canine intestinal spirochetosis and to characterize the canine isolates. Three canine isolates were weakly beta-hemolytic and had sharp end shape with 5 flagella. Isolates didn't produce indole but fermented fructose. In $API-ZYM^{(R)}$ study, isolates were alpha-glucosidase negative and alpha-galactosidase positive, which is the typical characteristics of B. pilosicoli. In multilocus enzyme electrophoresis(MEE) study, isolates were divided into 2 electrophoretic types. Isolates showed different properties with B. hyodysenteriae but closely related with pathogenic canine intestinal spirochete(B. pilosicoli). This is the first report of the isolation and characterization of canine intestinal spirochete in Korean dogs.
MC1R 유전자의 PCR-RFLP를 이용한 한우육과 젖소육/black Angus 수입육의 구분
김태중,이재일,Kim, Tae-Jung,Lee, Jae-Il 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.3
The melanocortin 1 receptor (MC1R) plays an important role in regulation of melanin pigment synthesis within mammalian melanocytes. Mutations within the gene encoding MC1R have been shown to explain coat color variations within several mammalian species including cattle. To develope a rapid and accurate method for the identification of Hanwoo, we performed a modified PCR-RFLP analysis of MC1R gene using single nucleotide polymorphism (SNP) within MC1R as a target. A size of 538 bp (537 bp for Hanwoo) was amplified by PCR, digested with Hpa II, and electrophoresed on a 1.5% agarose gel. A PCR product from Hanwoo showed a single band of 537 bp, whereas two fragments of 328 bp and 210 bp were detected in both Holstein and Black angus. The current result suggests that the PCR-RFLP using our primers and enzyme digestion system would be very accurate, easy and reproducible method to discriminate between Hanwoo and Holstein/Black angus meat.