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RISS 인기검색어
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- 저자 : 김정운(J.U, Kim) (검색결과 1 건)
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김주희(J.H, Kim),김정운(J.U, Kim),신길호(G.H, Shin),최형국(H.K, Choi),김선우(S.W, Kim) 한국차학회 1997 한국차학회지 Vol.3 No.2
For cutting in vitro, culture conditions of tea plants, monthly contamination rate, selection of disinfectants and infectant exposure time, were investigated. Monthly contamination rates were 12% in May, 15% in Feburuary, 32% in November and 37% in September in vitro conditions. Therefore, it was considered that optimum cutting time was from March to April. The disinfectant of 95% ethanol and 7--8% CaOCl treated was heavily contaminated by 8~9% than 95% ethanol and 25% NaOCl treated by 3% but in the latter case, survival rate was reduced and the disinfective degree was reduced in low concentration. Contamination rates on disinfectant exposure time were 10~16% in the 95% ethanol and CaOCl treated for 15~20min and decreased by 9~11% in the 95% ethanol and 25% NaOCl for 15~20min. but necrosis rate of the latter was 18~79% and survival rate was reduced. The optimum disinfection conditions in vitro culture tea plants are as follows ; after dip plant in the 95% ethanol for 3~5 seconds, disinfect by 7~8% CaOCl treated for 15~20min. and rinse in sterilized water, 3~5 time repeatly.
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