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RISS 인기검색어
- 검색키워드
- 저자 : 김석렬 외 (검색결과 4 건)
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Comparison of WSSV (white spot syndrome virus) DNA using Different oligonucleotide primers
김석렬 외 전남대학교 수산과학연구소 2006 수산과학연구소논문집 Vol.15 No.2
White spot syndrome (WSS) is a viral disease that affects on most of the commercially cultivated shrimp species in worldwide. WSSV causing white spot syndrome in Korea were genetically similar to PRDV in Japan. In this study, we compared PCR products of WSSV with those of PRDV, using their specific oligonucleotide primers. As a result of sequence analysis, amplified PCR products were almost identical to the described sequences used in this experiment. Therefore, the results obtained in this study suggest that WSSV causing white spot syndrome in Korea were genetically similar to PRDV in Japan. Naturally, more detailed studies will be needed to identify the taxonomic correlation between these viruses.
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김석렬 외 전남대학교 수산과학연구소 2006 수산과학연구소논문집 Vol.15 No.1
In this study, we were concentrating virus form natural seawater of near shrimp farms collected in the west and south coast and were detecting white spot syndrome virus (WSSV) from cultured shrimp using polymerase chain reaction (PCR). From Aug. 1999 to Aug. 2001, both of the cultured shrimp and concentrated seawater samples collected from Sinan, Kochang, Kangjin, and Sunchun could detect gene of WSSV. In Aug. 1999 samples, we could also detected WSSV in common brackish goby samples collected from shrimp farm of Kochang. All of the seawater samples and cultured shrimp collected from the west-south coast in 1999-2000 tested WSSV-positive, suggesting that WSSV is widely distributed in seawater of the west-south coast all time of the year.
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김석렬 외 전남대학교 수산과학연구소 2006 수산과학연구소논문집 Vol.14 No.1
In this study, we were concentrating virus form natural seawater collected from the south coast and were detecting fish pathogenic virus (Iridovirus) using polymerase chain reaction (PCR). In July 1999, all of the concentrated seawater samples collected from Sinan, Wando, Yeosu, Namhea and Tongyong could detect gene of iridovirus. In Oct. 1999 samples, we could also detected iridovirus in seawater samples collected from coast of Wando and Yosu. None of the seawater samples collected from Jan. to Apr. 2000 tested was positive for iridovirus. However all of the seawater samples collected from the south coast in July, 2000 tested iridovirus-positive, suggesting that iridovirus is widely distributed in seawater of the south coast when temperature of seawater is high in the season.
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Red Seabream Iridovirus (RSIV)의 가두리양식장내 감염동태
김석렬 외 전남대학교 수산과학연구소 2006 수산과학연구소논문집 Vol.14 No.2
Since 1998, Red seabream Iridovirus (RSIV) has been detected every year and has caused economic damages in net-cage cultured red seabream, Pagrus major and striped beakperch (Japanese parrotfish), Oplegnathus fasciatus (Temminck & Schlegel), in southern and western coastal areas of the Korean peninsula. This study was conducted to know epidemiological characteristics of the RSIV infection in net-cage farm. For the purpose, 116 red seabream and 89 striped beakperch were collected three fish farms in Namhae area during May to October in 2002. First diagnosis included field observations, gross and internal observations of fishes, Giemsa stain of the spleen and kidney stamp to identify enlarged cells which is a characteristic of the RSIV infection. In addition, virus culture by cell lines, histopathological studies and polymerase chain reaction (PCR) were conducted. In spring, RSIV was detected from the fish body but not in sea water. Although RSIV was exist in fish body no mortality was recorded. The mortality recorded from the August, and increased in September and October according with increasing of water temperature. During August and October, RSIV was detected not only dead fish but also sea water. Striped beakperch was more susceptible than red seabream against RSIV. RSIV could be transmitted via sea water and caused mortality in October to red seabream which is less susceptible to RSIV.
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