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한우 공란우의 일시적 저영양과 신체충실지수 조건에 따른 체내수정란 생산 효율
고응규(Yeoung-Gyu Ko),김남태(Nam-Tea Kim),이재영(Jae-Yeong Lee),이세영(Se Young Lee),김찬란(Chan-Lan Kim),조상래(Sang Rae Cho) 한국산학기술학회 2024 한국산학기술학회논문지 Vol.25 No.1
The study sought to investigate the in vivo embryos and the number of transferable embryos produced under transient hypo-nutrition conditions and with different body condition scores (BCS) in a multiple ovulation treatment applied to Hanwoo cows. Seventy cows were selected and categorized into control, high-nutrition, and low-nutrition groups based on their feeding levels, and the study was conducted for 23 days. The total number of recovered embryos per group was 6.87±1.34, 10.67±2.00, and 11.78±1.16, respectively. The number of transferable embryos per group was 4.40±1.02, 7.33±2.18, and 8.20±0.97, respectively. This was significantly higher (p<0.05) in the low-nutrition group than in the other two groups. The number of recovered embryos according to the BCS was 4.50±4.50 in the BCS 2.0 group, 11.19±1.34 in the BCS 2.5-3.0, and 14.58±2.38 in the BCS 3.5 group. The number of transferable embryos was 10.75±2.33 in the BCS 3.5 group, which was significantly higher (p<0.05) than 2.50±2.0 in the BCS 2.0 and 7.59±1.05 in the BCS 2.5~3.0 group. In conclusion, maintaining the BCS level of donors above 2.5 is recommended, and temporary hypo-nutrition treatment was found to be effective for fertilized egg production, especially in cows with BCS above 3.5.
한우에서 Ovum Pick-Up 유래 수정란의 임신 시기별 임신율 비교
고응규(Yeoung-Gyu Ko),이재영(Jae-Yeong Lee),김남태(Nam-Tea Kim),김찬란(Chan-Lan Kim),이세영(Se Young Lee),최창용(Changyong Choe) 한국산학기술학회 2023 한국산학기술학회논문지 Vol.24 No.7
본 연구는 한우에서 난소 내 난자 채취(Ovum Pick-Up, OPU) 기술을 적용하여 수정란이식을 실시하였을 경우 임신기간 동안 임신율을 어떻게 유지하면서 분만에 이르는지 확인하고자 수행하였다. 수정란이식 후 시기별 임신율은 45일령 50%, 2개월령 47.5%, 6.5개월령 35.0%였다. 신선 수정란과 동결 수정란의 임신율은 45일령, 2개월령, 4개월령, 6.5개월령에 신선 수정란 64.3%, 64.3%, 57.1%, 50.0%였으며 동결 수정란은 임신 전체 기간동안 16.7%를 나타내었다. 상실배, 초기배반포, 배반포기 수정란을 이식하였을 때 임신기간이 경과할수록 임신율이 떨어졌으며, 수정란의 발달 단계가 성숙한 것을 이식할수록 임신율이 높아지는 것을 확인하였다. 공란우별로 7∼8개의 수정란을 이식하고 이들의 임신율을 조사한 결과 이식 후 6.5개월령에서 임신율이 12.5%∼71.4%를 나타내어 공란우별로 임신율에 큰 차이를 나타내었다. 수란우의 임신율은 6.5개월령에서 미경산우 16.7%, 경산우 57.9%로 나타났다. 본 연구 결과 한우에서 OPU 유래 수정란을 이식하였을 경우 신선·동결 수정란, 수정란 발달 단계, 공란우 개체, 수란우 분만 경력에 따라 수정란이식 후 임신율에 차이가 나는 것을 확인하였는데, 이는 수정란이식 후 임신율 및 분만율에 다양한 요인이 작용하는 것을 확인하는 계기가 되었으므로, 본 연구를 바탕으로 한우의 수정란이식 임신율을 향상 시킬 수 있는 다양한 연구를 지속적으로 추진하여야 할 것으로 사료된다. This study investigates how to maintain the pregnancy rate and reach parturition subsequent to embryo transfer achieved by applying the Ovum Pick-Up (OPU) technology in Korean native cattle (Hanwoo). The overall pregnancy rates after embryo transfer were determined to be 50% at 45 days, 47.5% at 2 months, and 35.0% at 6.5 months after embryo transfer. The pregnancy rates obtained after AI for fresh and frozen embryos were 64.3%, 64.3%, 57.1%, and 50.0% for fresh embryos at 45 days, 2 months, 4 months, and 6.5 months, respectively, and 16.7% for frozen embryos during the entire period. Transferring morula, early blastocyst, and blastocyst stage embryos resulted in decreased pregnancy rate during the pregnancy period, whereas transferring embryos at a mature developmental stage resulted in increased pregnancies. In all, 7 to 8 embryos of each donor cow were cultured to the blastocyst stage and transferred to recipient cows. This resulted in increasing their pregnancy rates from 12.5% to 71.4%. At 6.5 months of gestation, the pregnancy rates obtained were 16.7% in heifers and 57.9% in multiparous cows. Results of this study indicate the need for continuous promotion of further research that will help improve the pregnancy rate after embryo transfer of Hanwoo cows.