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RISS 인기검색어
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SSR 마커를 이용한 복숭아 품종의 유전적 다양성 분석
홍지화(Jee-Hwa Hong),이승인(Seung-In Yi),권용삼9Yong-Sham Kwon),김영(Young Kim),최근진(Keun-Jin Choi) 한국육종학회 2013 한국육종학회지 Vol.45 No.3
The objective of this study was to evaluate genetic diversity in 72 major peach varieties by using SSR markers. A set of 189 SSR primer pairs was screened and 74 primer pairs showed polymorphism in 9 varieties. Twenty primer pairs out of 74 primer pairs showed clear band pattern and repetitive reproducibility. The relationship between 20 markers genotypes and 72 varieties was analyzed. A total of 71 polymorphic amplified fragments were obtained by using 20 SSR markers. Two to nine SSR alleles were detected for each locus with an average of 3.6 alleles per locus. Average polymorphism information content(PIC) was 0.523, ranging from 0.246 to 0.771. A total of 71 marker loci were used to calculate Jaccard’s distance coefficients for cluster analysis using UPGMA. Clustering group was largely divided 2 groups according to absence or presence of pubescence on the fruit surface and genetic distance of cluster ranging from 0.39 to 1.00. Analysis of genetic diversity revealed that these 20 SSR marker sets discriminated a total of 68 varieties except for 4 mutant varieties among 72 varieties. These SSR markers will be utilized as molecular evidence in variety identification of peach.
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