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집단게임놀이가 유아의 자기조절력과 유치원 적응행동에 미치는 효과
강명화 ( Myoung Hwa Kang ),박인전 ( In Jeon Park ) 한국놀이치료학회 2013 한국놀이치료학회지(놀이치료연구) Vol.16 No.3
이 연구는 유아의 자기조절력과 유치원 적응행동이 집단게임놀이를 통해 향상될 수 있는지를 검증하기 위해 고안되었다. 이 연구의 대상은 D광역시에 위치한 유치원의 만 5세아로, 20명은 집단 게임놀이를 실시하는 실험집단으로, 20명은 신체활동놀이를 실시하는 비교집단으로 선정하였으며, 20명은 통제집단으로 구성하였다. 각 그룹의 실험처치는 총 9주(주 2회) 동안 총 16회기에 걸쳐 실시하였다. 이 연구의 사전, 사후검사는 자기조절력 검사와 유치원 적응행동 척도를 사용하여 측 정하였다. 그리고 통계적 분석은 SPSS 19.0 program을 활용하여 공분 산 분석(ANCOVA)을 실시하여 프로그램의 효과를 검증하였다. 이 연구의 주요결과는 다음과 같다. 첫째, 집단게임놀이를 실시한 실험집단과 신체활동놀이를 실시한 비교집단 모두 유아의 자기조절력 향상에 효과가 있는 것으로 나타났다. 특히, 집단게임놀이를 실시한 집단이 신체활동놀이를 실시한 집단에 비해 전반적으로 효과가 더 높았다. 둘째, 유아의 유치원 적응행동 향상에 집단게임놀이를 실시한 실험집단과 신체 활동놀이를 실시한 비교집단 모두 효과가 있는 것으로 나타났다. 특히, 신체활동놀이보다 집단 게 임 놀이를 실시했을 때 전반적으로 효과가 더 높았다. This study sought to verify whether young children`s self-regulation and adaptive behaviors in kindergarten could be improved through group game play. The subjects of the study consisted of five-year-old kindergarteners in D Metropolitan City. Pre- and post-tests for this study were conducted using a self-regulation assessment and a scale of adaptive behaviors in kindergarten. In addition, ANCOVA was carried out using the SPSS 19.0 program in order to statistically prove the effect of the program. The research findings and conclusions were as follows: First, both the experimental group which had participated in group game play and the comparison group which had engaged instead in physical activity play showed improvement in terms of their self-regulation. Second, both the experimental group and the comparison group showed improvement in adaptive behaviors in kindergarten.
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중증 전자간증 태반 내 Junctional Protein에 대한 Ginsenoside Rk1의 효과
임승철 ( Seung Chul Lim ),맹용선 ( Yong Sun Maeng ),권자영 ( Ja Young Kwon ),강명화 ( Myoung Hwa Kang ),황정혜 ( Jeong Hye Hyang ),김영한 ( Young Han Kim ),권영근 ( Young Keun Kwon ),박용원 ( Yong Won Park ) 대한산부인과학회 2009 Obstetrics & Gynecology Science Vol.52 No.3
Objective: To investigate the differential expression of junctional proteins in the normal and preeclamptic human placenta and the effect of ginsenoside Rk1 in junctional proteins. Methods: Placental tissues from 10 women with severe preeclampsia and 5 normal women were collected at the time of their cesarean section. Five of 10 preeclamptic women were complicated with intrauterine growth restriction (IUGR). Immunohistochemistry and Western blotting was employed to localize junctional proteins (zo-1, occludin and plakoglobin) positive cells. The placental explant culture was performed to investigate if Rk1 can attenuate the expression of junctional proteins (zo-1, occluding and plakoglobin) induced by deferoxamine-induced hypoxia. Rk1 was treated at the day 3 and Western blot analysis was performed for protein quantification. Results: There was no different expression of zo-1 and plakoglobin among all the study groups. Occludin showed negative at the endothelial cells of the terminal villi in both normal and preeclampsia groups. At the endothelial cells of the stem villi, occludin was detected in both normal and severe preeclamptic placenta with normal fetal growth. However, severe preeclampsia with IUGR were decreased expression of occludin at the endothelial cells of the stem villi. When we administered Rk1 to the placenta treated with DFO, expression of occluding was not different. Conclusion: The placental expression of zo-1 and plakoglobin were not different among the study groups, while that of occludin was significantly decreased at the endothelium of stem villi in severe preeclampsia with IUGR. Rk-1 showed no effect on the placental junctional proteins. These results suggest that occludin may play a role in pathophysiology of fetal growth restriction in utero.
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정상 산모와 전자간증 산모의 태반에서 Adenosine 수용체의 발현 양상
김영한 ( Young Han Kim ),조남훈 ( Nam Hoon Cho ),황한성 ( Han Sung Hwang ),권자영 ( Ja Young Kwon ),강명화 ( Myoung Hwa Kang ),박용원 ( Yong Won Park ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.5
Objective: The aim of the study was to investigate the differential expression of adenosine receptors (ADORs) in the normal and preeclamptic placenta. Methods: Placentas were obtained from women undergoing cesarean section with normal and preeclamptic pregnancies at term. Total RNA was reverse transcribed using ADORs gene specific primers. RT-PCR measurements were made semi-quantitatively. Western blot analysis was performed for protein quantitation. Immunohistochemical staining with anti-adenosine receptor antibodies were employed to localize adenosine receptors in placental tissues. Results: RT-PCR revealed that A2aR, A2bR, and A3R mRNA, not A1 receptor mRNA were expressed in both normal and preeclamptic placenta. Interestingly, there were somewhat higher expressions of A2aR, A2bR, and A3R mRNA in preeclamptic placenta than in normal placenta. Western blotting revealed that A2a, A2b, and A3 receptors were all present in the placental tissue as verified by immunoreactive protein bands. The bands for the A2a, A2b, and A3 receptors were stronger in preeclamptic placenta than in normal placenta. A2a and A2b receptors were detected in endothelial cell, whereas we could not find the staining for A3 receptor in endothelial cells. Importantly, A3 antibody had high intensity of staining in trophoblasts in preeclampsia. Conclusion: To our knowledge, this study is the first to evaluate the expression of ADORs in normal placenta, and to compare ADOR subtypes in normal versus preeclamptic placenta. This study suggests that the specific subtype of ADORs may have a role in the development of preeclampsia.
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