담배吸煙이 口腔粘膜에 미치는 影響에 關한 實驗的 硏究

趙漢國(Han Guk Cho) 대한치과의사협회 1980 대한치과의사협회지 Vol.18 No.10

顎骨 齒性角化性囊腫의 臨床病理學的 硏究

趙漢國(Han Guk Cho) 대한치과의사협회 1986 대한치과의사협회지 Vol.24 No.5

담배가 미치는 口腔癌의 誘因性에 關한 組織化學的 硏究

趙漢國(Han Guk Cho) 대한치과의사협회 1981 대한치과의사협회지 Vol.19 No.10

韓國人 口腔癌의 發生狀況에 關한 病理學的 硏究

趙漢國(Han Guk Cho) 대한치과의사협회 1984 대한치과의사협회지 Vol.22 No.10

담배흡연이 구강점막에 미치는 영향에 관한 실험적 연구

조한국,Cho, Han-Guk 대한치과의사협회 1980 대한치과의사협회지 Vol.18 No.8

약물성 치은증식에서의 제Ⅰ,Ⅲ형 교원질 분포에 관한 면역조직화학적 연구

조영호,조한국 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.1

The purpose of this study was to evaluate the distribution of type I and type III collagen in drug-induced gingival hyperplasia, and then to prove differences in normal gingiva and drug-induced gingival hyperplasia. 2 cases of normal gingiva and fibroma as controls, 4 cases of dilantin gingival hyperplasia and nifedipine gingival hyperplasia from files of Dept. of Oral Pathology, School of Dentistry, Kyung Hee University were stained with H--E, Masson's trichrome for collagen, Verhoeff for elastic fiber, Toluidine blue for mast cells, andimmunostained with rabbit anti-human type I and type III collagen antibody using labeled streptavidin-biotin complex peroxidase method. The results were as follows ; 1. Age distribution showed that dilantin gingival hyperplasia and nifedipine gingival hyperplasia occurred in range of age from 13 to 39 years(mean age : 26.5 years) and from 33 to 57 years(mean age : 45.5 years), respectively. And sex distribution showed mastly more predilection for male. 2. The cases of dilantin gingival hyperplasia were weak positive immunoreactivity to anti-type I collagen antibody in covering epithelium and lamina propria, and strong positive immunoreactivity to anti-type III collagen antibody in lamina propria, perivascularand hyperplastic collagen bundles. 3. The cases of nifedipine gingival hyperplasia were moderate or strong positive immunoreactivity in hyperplastic covering epithelium to anti-type I collagen antibody, and strong positive immunoreactivity in subepithelial connective tissue, perivascular and hyperplastic collagen bundles to anti-type III collagen antibody but weak positive immunoreactivity in inflammatory region. 4. The elastic fibers were distributed in the area of hyperplastic collagen bundles and wall of blood vessels. 5. The mast cells were distributed in the subepithehal connective tissue, perivascular region and loose connective tissue. 6. The immunoreactivity to anti-type I , III collagen antibody were reduced in the inflammatory area withy prominent plasma cells.

N-nitroso-N-methylurea가 백서의 치배에 미치는 영향

한지현,조한국 慶熙大學校 齒科大學 1994 慶熙齒大論文集 Vol.16 No.1

The purpose of this study was to evaluate the effect of N-nitroso-N-methylurea(NMU Sigma Co.) on the tooth gem ,teeth as well as the periodontal tissue of the mother rats and also the teeth germs of the prenatal rats. Forty eight female Wistar rats, 200gm in body weight, were used in this experiment, in which pregnancy had been induced in all of them. In the experimental group of 32 a 0.4m1 NMU solution which was diluted in normal saline at a dose of lgm/100m1 was intravenously injected into the tail vein of all thirty two on the 13th day after pregnancy. Arid in the control group of 16, only 0.4m1 of normal saline solution was injected. The 48 pregnant rats were divided into 8 groups, each group consisting of 4 from the experimental group and 2 from the control group. One group was sacrificed on each of the following respective occasions; on the 17th, 19th, and 21rst day of pregnancy as well as on the 5th, 9th, 13th, 20th, and 24th week after delivery. When examined, the mandible, cervical lymph node, mammary gland, heart, liver, lung, kidney, ovary, stomach and colon were removed from moth and prenatal rats obtained from pregnant rats which were sacrificed during pregnancy. Then the histopathologica evaluation was performed by the routine method. The results were as follows; 1. The tooth germs of the mother rats began to form dysplastic dentin and irregular ameloblastic layer on the 21rst day, and metaplastic adenomatoid proliferation of the epithelium in some tooth germs with the time lapse. 2. The pulpal tissue of the mother rats revealed severe congestion of the blood vessels and a dense cellular layer odontoblasts, and then cystic structures and denticles was formed gradually 3. The periodontal tissue of the mothers was thickened by proliferation of fibroblasts with blood vessel congestion, and it showed formation of cysts and denticles in some portions and hypercementosis of molar teeth. The gingiva revealed hyperkeratinization and basilar hyperplasia by epithelial proliferation, but did not show dysplastic or malignant features 4. There was 1 mammary gland carcinoma, 3 lung carcinomas, 3 hepatic carcinomas, 2 kidney sarcomas and 2 colon carcinomas among all organs removed from mothers. 5. In the tooth germs at the cap stage of the prenatal rats, the inner enamel epithelium revealed metaplastic proliferation with hyperchromatic nuclei. 6. The tooth germs of the mothor rats showed more severe developmental disturbance than that of prenatal rats. 7. To conclude, the general administration of NMU induced carcinoma on various organs and nietaplastic adenomatoid proliferation of the epithelium in some tooth germs can be recognized as an early developing feature of odontogenic carcinoma

치성각화성낭종에서의 발암성에 관한 살험적 연구

백진기,조한국 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.1

The purpose of this study was to observation the pathogenesis and carcinogenesis of lining epithelium of odontogenic keratocyst which injected with DMBA ( 9,10-dimethyl-1,2 -benzanthracene, Sigma Chemical Co.) after ;;nplantation of upper gingival epithelium of rats. Bony cavity was formed in the left mandibular body area of Sixty Sprague-Dawley rats with #3 round bur and then gingival epithelium was implanted to be inner surface. Six experimental animals of group 1 were sacrified at 12,3,4,6 weeks after implantation respectively. Thirty experimental animals of group 2 were injected with DMBA around the bony cavity at 6 weeks after implantation and then six experimental animals of group 2 were sacrified at 2,4,6,8,10 weeks after injection with DMBA respectively. And the formalin-fixed paraffin embedded sections were stained as H-E staining routinely, PAS staining for glycogen, and Ayoub-Shklar staining for keratinization, and then observed with light microscope. The obtained results were as follows 1. In group 1, gingival implantation-induced cyst-like lesion was composed of thin lining epithelium and connective tissue wall at 4 weeks, and formed odontogenic keratocyst at 6 weeks. 2. In group 2, odontogenic keratocyst revealed inflammatory response by DMBA injection at-2 weeks, basilar hyperplasia of lining epithelium at 4-6 weeks, and carcinoma in situ at 8 weeks. After all odontogenic keratocyst proceeded to odontogenic carcinoma showing highly or well differentiated squamous cell carcinoma at 10 weeks. 3. The lining epithelium of odontogenic keratocyst was infiltrative growth and the cystic wall accompanied with fibrosarcoma and rhabdomyosarcoma at 8 weeks in group 2. 4. The epithelial island inferred as ameloblastomatous changes were found around the tooth germ of incisor teeth, and did adenoma-like changes in minor salivary gland and hypercementosis in molar teeth. 5. In PAS reaction, prickle cell layer and cystic wall revealed strong reactivity, and weak or negative reactivity toward poorly differentiated cells. In Ayoub-Shklar reaction, strong reactivity was revealed in prickle cell layer of lining epithelium and area of dysplastic proliferation.

Ethylnitrosourea가 치배 및 치주조직에 미치는 영향에 관한 실험적 연구

오창근,조한국 慶熙大學校 齒科大學 1994 慶熙齒大論文集 Vol.16 No.1

The purpose of this study was to evaluate the effect of ethylnitrosourea(N-ethyl-N-nitrosourea ; ENU, Sigma Co.) on the tooth germs and its surrounding tissues. Forty male Wistar rats; one month of age and 100-130gm in body weight, were used in this experiment, which were devided into 3 experimental groups and 2 control groups. Ten animals of experimental group 1 were given local injection of 1% ENU solution which were dissolved in physiologic saline into the region of incisor tooth germ of the right mandible every other day for 19 days (a total of 10 times). Ten animals of experimental group 2 received ENU injections as the same manner as group 1 and were given mechanical injuries on the same regions at 2nd, 8th and 15th days after beginning of experiment. And fifteen animals of experimental group 3 received mechanical injuries as well as ENU injections as the same manner of group 2, and were given 4% ethanol as drinking water and subcutaneous injections of 2.5mg prednisolone acetate at 2 times per week. Three animals a control group 4 were treated as the same manner with experimental group 3, but received injections of physiologic saline instead of ENU, and two animals of control group 5 were not treated. All animals were sacrificed at the end of the sixth month, and the mandibles were removed, fixed in 10% N-fornialin, decalcified with electrolytic decalcification method, embedded in paraffin, sectioned in 4-6/tm thickness, and stained with Hematoxylin and eosin, and Masson's trichrome. The results were as follows ; 1. Various polycystic structures with fibrous connective tissue wall and neoplasm-like proliferation were revealed at the ENU-injected areas of the tooth germs, the periodontal ligaments and the salivary glands. 2. The epithelium of the tooth germ revealed irregular epithelial hyperplasia and disturbance of dentinogenesis. 3. The stratum intermedium of the tooth germ revealed severe adenomatous proliferations, therefore suggesting a potential of odontogenic tumors. 4. Malassez's epithelial rests in the periodontal membrane of the posterior teeth revealed mild hyperplastic proliferation. 5. Gingival hyperplasia at the posterior teeth and the formation of epithelial islands resembling with enamel organ at the affected interdental papillae were observed. 6. The ductal epithelium of the intercalated ducts and the secretory ducts of the accessory salivary glands revealed basal cell adenomatous proliferations, 7. Group 2 and 3 revealed more prominent histopathological changes than group 1, therefore it suggested that these changes would develop to odontogenic tumors.

β-carotene과 Protein-bound polysaccharide(PS-K)가 악골 이식암에 미치는 영향에 관한 실험적 연구

어영선,조한국 慶熙大學校 齒科大學 1993 慶熙齒大論文集 Vol.15 No.1

The purpose of this study was to evaluate the inhibitory effect of 03-carotene and PS-K on the oral transplanted tumor by the transplantation of Sarcoma 180 ascite tumor cells in the bone of incisor tooth germ region of the right mandible. Three week-aged 56 male mice(ICR), weighing 20gm, were devided to 4 experimental groups and 2 control groups. Experimental group 1(12 mice) was transplanted 0.05ml Sarcoma 180 ascite tumor cells per a mouse with 25 Gauge syringe. Group 2(12 mice) was supplied orally 3 times per week with 1-2m1(3-carotene that was dissolved in soybeam milk after transplantation of Sarcoma 180. Group 3 (12 mice) was administered orally 3 times per week with 2m1 PS-K solution that was dissolved in soybeam milk at a dosage of lgm/200ntl after transplantation. Group 4(12 mice) administered 0-carotene and PS-K with the same method. Control group 5(6 mice) was administered 0-carotene and PS-K only and group 6(2 mice) was untreated control. Five mice of each experimental group and 1 mouse of group 5 were sacrificed at 4, 6, 8, 10, 12, 14 days after experimentation. Then the mandible was removed, fixed in 10°N-Formalin and decalcified in 5% nitric acid. And embedded in paraffin; sectioned to 4― 6?m thickness, and stained with Hematoxylin and Eosin, Masson's trichrome and Van Gieson by the usual method; The results were as follows 1. Sarcoma 180 transplanted group revealed inflammatory reaction, degeneration and necrosis of transplanted region, located at 4 days, active malignancy of tumor cells at 6 days, infiltration into the alveolar bone and the periodontal membrane at 8 days, infiltrative proliferation into the periapical portion, adjacent muscular tissue, salivary gland and deep jaw bone at 10, 12 days. 2. The growth of the transplanted tumor cells was inhibited slightly in 0-carotene administered group, morelyinhibited in PS-K administered group and markedly inhibited in 0-carotene and PS-K combined group. 3. There are marked infiltration of lymphocytes due to exudative inflammatory reaction in the adjacent tissue to the transplanted tumor cells. 4. Control group were administered 0-carotene and PS-K only, revealed congestion of blood vessels at 4 days, edematous and hemorrhagic features at 8, 10 days.