근·현대 유적 조사연구 현황과 중요성

최종규 중부고고학회 2019 중부고고학회 학술대회논문집 Vol.2019 No.1

인천시 산단 주변지역 대기 중 유해 휘발성유기화합물의 특성에 관한연구

최종규,서성녀,김현기,이현주,방준상,강희규,이창근,김주원,이승묵,조경덕 한국대기환경학회 2012 한국대기환경학회 학술대회논문집 Vol.2012 No.5

Urease gene의 전이에 의한 길항세균 Bacillus sp. SH14의 길항능력 증가

최종규,김상달 한국산업미생물학회 1998 한국미생물·생명공학회지 Vol.26 No.2

최근 생물방제균으로 주목받고 있는 Enterobacter cloacae의 방제기작이 이 균에 의해 토양내에서 생산된 휘발성 ammonia이며 ammonia의 생산에는 urease가 관계한다는 보고를 근거로하여, 항생물질 생산성 균주로 선발된 우수한 길항균주에 암모니아 생성능, 즉 urease 유전자를 유전적으로 부가함으로써 항진균성 길항물질 생산과 암모니아 생산이 동시에 이루어 질 수 있는 새로운 다기능의 생물방제균을 유전적으로 육종하고자 하였다. 저병해 인삼경작지로부터 식물근부균 Fusarium solani의 생육을 강하게 억제하는 길항세균 한 균주 SH14 균주를 분리, 선발하였으며, 분리된 균주를 동정한 결과 Bacillus subtilis이거나 그 근연종으로 추정되었다. 억제기작 실험을 통해 길항균주 B. subtilis SH14에 의해 생산되는 항진균성 길항물질은 외막가수분해효소와 같은 고분자 물질이 아니라 열에 안정한 저분자의 항생물질임을 알 수 있었다. 한편 ammonia 생산을 위한 urease의 유전자는 urease 생산력이 강력한 호알칼리성 Bacillus pasteurii의 urease 생산유전자를 E. coli-Bacillus shuttle vector인 pEB203에 subcloning하였고, 이어서 pGU 366으로 명명된 이 recombinant plasmid를 선발된 항진균성 길항균주 B. subtilis SH14에 PEG-induced protoplast transformation 방법으로 도입, 발현시켰으며, 최적조건을 조사하여 90분간의 lysozyme 처리과정 후 1.5 ㎍/㎖의 DNA와 40% PEG4000의 첨가로 약 6.5×10 exp (4)의 형질전환율을 얻을 수 있었다. 아울러 암모니아 생성능이 부가된 생물방제균 B. subtilis SH14(pGU366)에 의해 식물근부균 F. solani에 대한 생육억제력이 증가되는지 여부를 억제거리 측정법과 균체중량법을 통해 확인한 결과 urease 유전자가 도입된 형질전환체 B. subtilis SH14(pGU366)의 근부균 생육억제능이 각각 36.7%, 44.0%정도로 숙주균주인 B. subtilis SH14에 비해 근부균 생육억제능을 보다 강하게 나타내었음을 알 수 있었다. 따라서 항진균성 항생물질 생산성 생물방제균 B. subtilis SH14에 외부의 urease 유전자를 도입하여 ammonia 생성능을 부가함으로써 생물방제력의 상승효과를 거둘 수 있었다. It were reported that antifungal mechanism of Enterobacter cloacae is a volatile ammonia that produced by the strain in soil, and the production of ammonia is related to the bacterial urease activity. A powerful bacterium SH14 against soilborne pathogen Fusarium solani, which cause root rot of many important crops, was selected from a ginseng pathogen supressive soil. The stain SH14 was identified as Bacillus subtilis by cultural, biochemical, morphologica method, and API test. From several in vitro tests, the antifungal substance that is produced from B. subtilis SH14 was revealed as heat-stable and low-molecular weight antibiotic substance. In order to construct the multifunctional biocontrol agent, the urease gene of Bacillus pasteurii which can produce pathogenes-supressive ammonia transfered into antifungal bacterium. First, a partial BamH I digestion fragment of plasmid pBU11 containing the alkalophilic B. pasteurii 11859 urease gene was inserted into the BamH I site of pEB203 and expressed in Escherichia coli JM109. The recombinant plasmid was designated as pGU366. The plasmid pGU366 containing urease gene was introduced into the B. subtilis SH14 with PEG-induced protoplast transformation (PIP) method. The urease gene was very stably expressed in the transformant of B. subtilis SH14. Also, the optimal conditions for tranformation were established and the highest transformation frequency was obtained by treatment of lysozyme for 90 min, and then addition of 1.5 ㎍/㎖ DNA and 40% PEG4000. From the in vitro antifungal test against F. solani, antifungal activity of B. subtilis SH14(pGU366) containing urease gene was much higher than that of the host strain. Genetical development of B. subtilis SH14 by transfer of urease gene can be responsible for enhanced biocontrol efficacy with its antibiotic action.

생물방제균 Bacillus subtilis YB-70의 외부 Urease 유전자 도입과 길항력 증강

최종규,김용수,이은탁,김상달 한국산업미생물학회 1997 한국미생물·생명공학회지 Vol.25 No.1

토양내 미생물개체군 중 우점화 능력이 강한 길항균주에 타 길항기작을 추가적으로 부가하여 다기능적 생물방제균을 육종하고자 하였다. 이를 위해 최근 생물방제균으로 주목받고 있는 Enterobacter cloacae의 길항기작이 이 균에 의해 토양내에 생산되는 휘발성 암모니아이었다는 보고를 근거로 하여, 이미 일련의 식물근부균 생물방제연구에서 선발된 식물방제균 Bacillus subtilis YB-70에 암모니아 생산에 필요한 urease 유전자를 유전적으로 부가함으로써 식물근부균 F. solani에 대한 길항력이 훨씬 강화된 생물방제균을 육종할 수 있었다. Urease 유전자는 토양세균 중 urease 생산능이 가장 큰 Bacillus pasteurii의 urease gene library (10.7 Kb)를 B. subtilis YB-70에 가장 잘 발현되는 plasmid vector pGB215-110의 HindⅢ site에 삽입하였으며, 이렇게 제작된 urease 함유 recombinant plasmid (pGU266)을 생물방제균 B. subtilis YB-70에 alkali cation competent transformaiton으로 도입시켰다. 이때 transformation의 최적 조건을 조사한 결과 PEG 40% 존재하에 2.5 ㎍/㎖의 DNA첨가로 가장 높은 형질전환율을 얻을 수 있었으며 일단 도입된 urease 유전자는 상당히 안정하게 B. subtilis YB-70 내에서 유지되었으며, 그 transformation된 recombinant plasmid의 존재도 Birnboim방법으로 agarose gel 상에서 확인할 수 있었다. 또한 urease 유전자가 도입된 형질전환체 B. subtilis (pGU266)은 urease gene이 도입되지 않은 B. subtilis YB-70에 비해 식물근부균 F. solani에 대한 길항력이 훨씬 증강됨도 확인되었다. To genetically breed powerful multifunctional antagonistic bacteria, the urease gene of alkalophilic Bacillus pasteurii was transferred into Bacillus subtilis YB-70 which had been selected as a powerful biocontrol agent against root-rotting fungus Fusarium solani. Urease gene was inserted into the HindⅢ site of pG215-110 and designated pGU266. The plasmid pGU266 containing urease gene was introduced into the B. subtilis YB-70 by alkali cation transformation system and the urease gene was very stably expressed in the transformant of B. subtilis YB-70(pGU266). The optimal conditions for the transfomation were also eveluated. From the in vitro antibiosis tests against F. solani, the antifungal activity of B. subtilis YB-70 containing urease gene was much efficient than that of the non-transformed strain. Genetic improvement of B. subtilis YB-70 by transfer of urease gene for the efficient control seemed to be responsible for enhanced plant growth and biocontrol efficacy by combinding its antibiotic action and ammonia producing ability.

생물방제균 Bfacillus subtilis YB-70의 외부 Urease 유전자 도입과 길항력 증강

최종규,김용수,이은탁,김상달,Choi, Jong-Kyu,Kim, Yong-Su,Lee, Eun-Tag,Kim, Sang-Dal 한국미생물 · 생명공학회 1997 한국미생물·생명공학회지 Vol.25 No.1

To genetically breed powerful multifunctional antagonistic bacteria, the urease gene of alkalophilic Bacillus pasteurii was transferred into Bacillus subtilis YB-70 which had been selected as a powerful biocontrol agent against root-rotting fungus Fusarium solani. Urease gene was inserted into the HindIII site of pGB215-110 and designated pGU266. The plasmid pGU266 containing urease gene was introduced into the B. subtilis YB-70 by alkali cation transformation system and the urease gene was very stably expressed in the transformant of B. subtilis YB-70(pGU266). The optimal conditions for the transfomation were also evaluated. From the in vitro antibiosis tests against F. solani, the antifungal activity of B. subtilis YB-70 containing urease gene was much efficient than that of the non-transformed strain. Genetic improvement of B. subtilis YB-70 by transfer of urease gene for the efficient control seemed to be responsible for enhanced plant growth and biocontrol efficacy by combining its astibiotic action and ammonia producing ability.

中期古墳의 性格에 대한 약간의 考察

崔鍾圭 부산대사학회 1983 역사와 세계 Vol.- No.7

The burial system of southern Korea in the 5th century characterized by the appearance of stone file tombs in Kyongju and large subterranean stone-chamber tombs in the other region. These are very different from the wooden chamber tombs which belong to previous period. And the burial goods of these tombs (5th century) are composed of new artifacts, for example cavalry armours, horse-equipments, metal works, which have similarity to those of Koguryo. At this point, it is supposed that the origin of those new artifacts was derived from Koguryo. The historical moment which brought the inroduction of Koguryo line artifacts to southern Korea is thought to have been the Conquest of king Kwang-gaeto on A. D.400. Because the beginning date of Koguryo line artifacts in southern Korea can't antedate A.D. 5th century.

$\beta$-시클로덱스트린($\beta$-Cyclodextrin)의 결합 특성과 벤젠의 생물학적 분해에의 적용에 대한 연구

최종규,손현석,조경덕 한국환경보건학회 2002 한국환경보건학회지 Vol.28 No.5

Recently, surfactants were frequently used in order to desorb the hydrophobic organic compounds (HOCs) from soil and to enhance the bioavailability. Among them, -cyclodextrin ($\beta$-CD) is one of those. This study was performed to investigate the binding characteristics between benzene and $\beta$-CD and to examine the bioavailability of benzene. First, we investigated binding characteristics between benzene and $\beta$-CD in water and water/soil system. Then, we examined the effect of $\beta$-CD on the biodegradation of benzene in water and water/soil system. Experimental results on the binding characteristics showed that $\beta$-CD resulted in an efficient complex formation with benzene. As -CD concentration increased, the benzene concentration complexed with $\beta$-CD rapidly increased to 30-40% initial benzene added, and reached the equilibrium. We also investigated the effect of $\beta$-CD on the desorption of benzene from soil in the water/soil system. As $\beta$-CD concentration increased, benzene concentration desorbed into water increased up to 90%. How-ever, in its application to biodegradation of benzene in water and water/soil system, the biodegradation rate of benzene did not improved in the presence of $\beta$-CD compared with in the absense of $\beta$-CD. This result indicated that $\beta$-CD was more preferentially used as a carbon source than benzene. Therefore, for remediation of benzene contaminated soils, $\beta$-CD can be used as a surfactant to desert benzene from soil, and then ex-situ chemical treatment can be applied for the remediation.

ESCO투자사례-노후 호텔 냉난방 기계 설비 교체

최종규,Choe, Jong-Gyu 에너지절약전문기업협회 2006 esco Vol.38 No.-

대북 민간건설공사의 위험대응방안에 관한 사례연구

최종규,김영애,송창영,김용수 대한건축학회지회연합회 2007 대한건축학회연합논문집 Vol.9 No.4

Different from governmental/government-financed construction projects, private construction projects in North Korea are placed in a complex and specific circumstance, thus being involved with a lot of risks. In this vein, it is necessary to take measures to manage such risk factors, but not many private construction projects in North Korea have been carried out so far and data and preceding studies on its risk management plans could be hardly found. Therefore, the purpose of this study was to examine the risks of private construction projects in North Korea and suggest some risk management plans through some examples. For this, the investigator interviewed with people who were working for constructors and their collaborators, and used data on the training of government officials. Study findings are as follows: 1)from planning to completion, risk factors of construction projects in North Korea were analyzed and measures were presented, centering on the approval of construction, shipping of materials, visiting procedures, stays, on-site construction, and safety supervision. 2) since the risk factors of private construction projects in North Korea are quite different from those of general construction projects, it is necessary to talk over a contract with the said party in North Korea fully in advance. 3) since the construction projects in North Korea are carried out in a specific site with which regular contacts are impossible, thorough advanced preparation is a must in order not to face the risks of cost and time.

헌책방 순례기

최종규 한국외국어대학 학도호국단 1995 외대 Vol.37 No.1