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- 저자 : Žarko Ivanović (검색결과 2 건)
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Žarko Ivanović,Tatjana Perović,Tatjana Popović,Jovana Blagojević,Nenad Trkulja,Snježana Hrnčić 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.1
Citrus blast caused by bacterium Pseudomonas syringaeis a very important disease of citrus occuring inmany areas of the world, but with few data about geneticstructure of the pathogen involved. Consideringthe above fact, this study reports genetic characterizationof 43 P. syringae isolates obtained from planttissue displaying citrus blast symptoms on mandarin(Citrus reticulata) in Montenegro, using multilocus sequenceanalysis of gyrB, rpoD, and gap1 gene sequences. Gene sequences from a collection of 54 referencepathotype strains of P. syringae from the Plant Associatedand Environmental Microbes Database (PAMDB)was used to establish a genetic relationship with ourisolates obtained from mandarin. Phylogenetic analysesof gyrB, rpoD, and gap1 gene sequences showedthat P. syringae pv. syringae causes citrus blast inmandarin in Montenegro, and belongs to genomospecies1. Genetic homogeneity of isolates suggested thatthe Montenegrian population might be clonal whichindicates a possible common source of infection. Thesefindings may assist in further epidemiological studiesof this pathogen and for determining mandarin breedingstrategies for P. syringae control.
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Genetic Diversity of Orobanche cumana Populations in Serbia
Ž,arko Ivanović,Dragana Marisavljević,Radovan Marinković,Petar Mitrović,Jovana Blagojević,Ivan Nikolić,Danijela Pavlović 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.6
In this study, we report genetic characterization of Oro- banche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analy- ses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum span- ning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.
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