유산구균 1370의 수용성 글루캔 형성에 영향을 미치는 인자

오인근,양규호,정진,오종석 대한소아치과학회 2000 大韓小兒齒科學會誌 Vol.27 No.2

Streptococcus mutans가 치태를 형성할 때, 유산구균 1370(Lactococcus lactis 1370)이 생산하는 수용성 글루캔이 영향을 미친다. 본 연구에서는 여러 인자에 의한 수용성 글루캔 형성 정도를 유산구균 1370 배양 상청액의 흡광도로 측정하여 다음과 같은 결과를 얻었다. 1. 5% 자당이 첨가된 M17 broth에서 유산구균 1370 배양 상청액의 흡광도는 높고 Streptococcus mutans 배양 상청액의 흡광도는 낮았으나, 통계학적인 유의성은 없었다. 2. M17 broth에 10% 자당을 첨가하여 유산구균 1370을 배양할시 배양 상청액의 흡광도는 첨가하지 않을 때보다 높았다(p〈0.05). 배지 pH가 5에서보다 7에서 배양 상청액의 흡광도가 더 높았다(p〈0.05). 3. 32℃, 37℃, 42℃중 37℃에서 배양시 배양 상청액의 흡광도가 가장 높았으나 통계학적인 유의성이 없었고, 호기성 배양시보다 혐기성 배양시 배양 상청액의 흡광도가 더 높았다(p〈0.05). 4. 배지의 CaCl₂농도가 1.0mM에서 (p〈0.05), KCl 농도가 2.5mM에서 (p〈0.05) 배양 상청액의 흡광도가 높았다. 5. M17 broth에 5% 자당을 첨가한 배지에 유산구균 1370을 접종하여 배양한 배양 상청액의 배지를 1:3으로 가한 비커와이어 검사에서 Streptococcus mutans에 의하여 형성된 인공치태 무게는 5.6mg으로 5% 자당을 첨가하지 않을 때의 103.0mg에 비교하여 현저히 감소하였다 (p〈0.05). 이상의 결과를 요약하면 유산구균 1370에 의한 수용성 글루캔의 형성은 자당이 함유된 배지나 세균 증식이 잘 되는 조건에서 증가하였으며, 수용성 글루캔이 Streptococcus mutans에 의한 인공치태 형성을 억제하였다.

주거단지 외부공간의 친환경성 평가에 관한 연구 : 서울 신시가지와 신도시의 공공주거단지를 중심으로 Focused on the Case of Public Housing Estates in Seoul and New Towns

오수호,장선영,김석경,이규인 대한건축학회 2004 대한건축학회논문집 Vol.20 No.6

This study aims at assessing the status of environment-friendly outdoor spaces in housing estates. For this purpose, 62 housing estates were selected : 28 housing estates from 3 new residential districts of Gaepo, Mockdong, and Sanggae in Seoul, and 34 housing estates from 3 new towns of Gwacheon, Bundang, and Joongdong around Seoul. As an assessment tool, the indices and standard from 'Land Use and Transportation field' and 'Ecological Environment field' of 'Green Building Certification' were used. The result of evaluation was that the quality of outdoor environment in Gwacheon was very high, and the next one was Bundang, Gaepo, Sanggae, Mockdong, and Joongdong in order. In 'Land Use and Transportation field', many housing estates in Mockdong got high scores, and in 'Ecological Environment field', Gwacheon made a good score.

치태형성 억제세균의 분리

오종석,박진경,양규호,정진 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.3

치태의 주요성분인 비수용성 글루캔의 형성을 억제시키는 세균을 분리하기 위하여 유치원 원아 만여명으로 부터 타액을 채취하였다.일회용 큐벧을 사용하여 비수용성 글루캔의 형성을 억제시킨 세균을 분리하였다.분리된 세균을 그람 염색과 API 20S kit 와 API 50 CHL kit를 사용하여 당발효 및 생화확적 특성을 검사한 결과,Streptococcus oralis,Streptococcus mitis,Streptococcus mitior,Streptococcus sanguis,Enterococcus durans,Lactobacillus lactis로 동정되었다.비수용성 글루캔 형성 억제 정도를 판정하기 위하여 일회용 큐벧에서 S.mutans 단독 배양시에는 550nm에서의 흡광도가 1.503이었으나,S.mutans 와 Streptococcus oralis,Streptococcus mitis,Streptococcus mitior,Streptococcus sanguis,Enterococcus durans,Lactobacillus lactis, Lactobacillus acidophilus 혼합 배양시에는 각각의 흡광도가 0.823,0.912,0.894,0.878,0.753,0.845,1.021로 감소되었다. The insoluble glucan is the major substance of dental plaque. In order to isolate the bacteria inhibiting the formation of insoluble glucan in disposable cuvette,saliva was got from about 10 thousand children.The isolated bacteria were tested by API 20S kit and API 50 CHL kit.These bacteria were identified as Streptococcus oralis,Streptococcus mitis,Streptococcus mitior,Streptococcus sanguis,Enterococcus durans,Lactobacillus lactis,and Lactobacillus acidophilus.When Streptococcus mutans was cultured with Streptococcus oralis,Streptococcus mitis,Streptococcus mitior,Streptococcus sanguis,Enterococcus durans,Lactobacillus lactis,and Lactobacillus acidophilus in disposable cuvette,the optical density at 550 nm was 0.823,0.912,0.894,0.878,0.753,0.845,1.021 respectively,while being 1.503 in the disposable cuvette Streptococcus mutans only.

유산구균 1370의 수용성 글루캔 형성에 영향을 미치는 인자

오인근,양규호,오종석 전남대학교 치과대학 2000 전남치대논문집 Vol.12 No.1

The water-soluble glucan prodeced by Lactococcus lactis 1370 affects the formation of dental plaque by Streptococcus mutans. In this study the factors affecting the formation of water-soluble glucan were assessed as the optical density of culture supernatant of Lactococcus lactis 1370 in the spectrophotometer. 1. The optical density of culture supernatant was high when Lactococcus lactis 1370 was cultured in M17 broth with 5% sucrose, while being low in culture supernatant of Streptococcus mutans. 2. The optical density of culture supernatant was higher when Lactococcus lactis 1370 was cultured in M17 broth with 10% sucrose than when being cultured without sucrose (p<0.05), and was higher at pH 7 than pH 5 (p<0.05). 3. The optical density of culture supernatant was the highest at 37℃ among 32℃, 37℃ and 42℃, and was higher in the anaerobic incubator than in the aerobic incubator (p<0.05). 4. The optical density of culture supernatant was the highest in the media containing 1.0mM CaCl2 (p<0.05), 2.5mM KCl (p<0.05), and 1.6 mM MgCl2. 5. When Streptococcus mutans was cultured in the media containing a quarter culture supernatant of Lactococcus lactis 1370 grown in M17 broth, the mean weight of produced artificial plaque was 103.0 mg on the wire, whereas being significantly reduced to 5.6mg in the media containing a quarter culture supernatant of Lactococcus lactis 1370 grown in M17 broth containing 5% sucrose (p<0.05). These results indicate that the water-soluble glucan is more formed by Lactococcus lactis 1370 in the media containing sucrose or under the adequate conditions for the growth of bacteria, and inhibits the formation of artificial plaque by Streptococcus mutans.

Fusobacterium nucleatum의 유황화합물 생성에 영향을 미치는 인자

오인근,박은혜,양규호,오종석 전남대학교 치의학연구소 2003 구강과학 Vol.15 No.1

Fusobacterium nucleatum, one of the bacteria causing halitosis, produces the volatile sulfur compounds (VSC) such as H2S in the media containing sulfur components, and forms FeS by binding with iron component. The various factors of oral cavity affect the concentration of sulfur compounds produced by Fusobacterium nucleatum. In this study, the effect of nutrients and pH on the production of sulfur compounds by Fusobacterium nucleatum was studied with the following results; 1. The optical density of broth was increased to 0.817±0.032 and 1.297±0.024 by adding 1.0% sodium thiosulfate and 0.05% L-cysteine hydrochloride in the media, respectively. 2. Though the optical density of broth was 0.799 ± 0.032 by adding volatile sulfur compounds (VSC) only in the media, it was increased to 1.775±0.003 and 1.648±0.022 by adding xylitol combined with glucose and fructose, respectively. 3. The concentration of VSC was above 20,000 ppb in the media above pH 5.5. The optical density of broth was still high in the media with L-cysteine hydrochloride of higher concentration, being low in the media of lower pH. 4. The concentration of VSC was high when there was distilled water or saline solution on the media, and their amount was small. These results suggest that the production of sulfur compounds by Fusobacterium nucleatum is inhibited by xylitol and acid.

Streptococcus oralis 의 인공치태 억제효과에 대한 연구

오종석,정성수,양규호,김선미 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.1

이 연구는 치아우식증이 주요 원인균인 Streptococcus mutans의 치태형성과 증식에 대한 Streptococcus oralis의 영향을 조사하고자 하였다. 이를 위해 S.mutans와 S.oralis 혼합 배양후 형성된 치태 무게와 생균수를 측정비교하였다. 또한 치태형성과 생균수에 영향을 미치는 요소들에 대해 조사하여 다음과 같은 결과를 얻었다. 1. S.mutans와 S.oralis의 혼합배양시 S.mutans 단독배양에 비해 치태무게와 생균수가 감소되었다. 2. S.mutans 단독배양에 10 mM의 표도당을 첨가한 군은 첨가하지 않은 군보다 치태무게가 증가되었으나,S.mutans와 S.oralis의 혼합배양에 10 mM의 포도당을 첨가한 군은 첨가하지 않은 군과 차이가 없었다. 3. S.mutans 단독배양에 10 mM S.mutans와 S.oralis 10 mM의 과당을 첨가한 군도 첨가하지 않은 군보다 치태무게가 증가되었다. 4. S.mutans와 S.oralis의 혼합농도를 달리하여 배양했을때 S.oralis의 혼합농도가 높아질수록 치태무게와 생균수는 감소되었다. 5. 배양기산(6,9,12,15시간)에 따른 치태무게와 생균수는 S.mutans단독배양에 비해 S.mutans와 S.oralis혼합배양시 배양 12시간 이후 급격히 감소되었다. 6. S.mutans와 S.oralis의 혼합배양에 H₂O₂를 소비하는 Staphylococcus epidermidis를 첨가한 군은 첨가히자 않은 군보다 치태무게와 생균수가 증가되었다. 이상의 결과로 S.oralis는 S.mutans와 혼합배양시 S.mutans의 증식과 인공치태의 생성을 억제함을 알 수 있었고, 이는 S.oralis가 H₂O₂를 분비함으로써 얻어진 결과임이 시사되었다. This study was performed to evaluate the effect of Streptococcus oralis on the formation of artificial plaque and the replication of Streptococcus mutans.S.mutans was incubated alone and in the combination with S.oralis in the beaker with wires. The produced plaque weight and the viable cells of S.mutans were compared between those cultures. Various factors were studied about the effect on the formation of plaque and the replication of S.mutans. Followings are the results. 1. Lower amount of plaque was produced and fewer cells of S. mutans were replicated at the mixed culture of S. mutans and S. oralis than S. mutans alone. 2. When 10 mM glucose was added, the plaque weight was increased in the culture of S. mutans alone. But in the mixed culture of S. mutans and S. oralis, the plaque weight was not increased when 10mM of glucose was added. 3. When 10 mM fructose was added, the plaque weight was increased in culture of S. mutans alone or combined S. mutans and S. oralis. 4. In the mixed culture of S.mutans and S. oralis with different concentration, the more S.oralis exist, the less plaque and the fewer viable cells of S. mutans were observed. 5. The plaque weight and the viable cells of S.mutans were more decreased in the mixed culture of S. mutans and S. oralis than S. mutans alone after 12 hours. 6. When Staphylococcus epidermidis consuming hydrogen peroxide was added to the mixed culture of S. mutans and S. oralis, the plaque weight and the viable cells of S. mutans were increased. These results indicate that S. oralis inhibited the formation of plaque and the replication of S. mutans,and this may result from the formation of hydrogen peroxide by S. oralis.

해조류에서 추출한 SCH-T3의 elastase 억제

오경석,박영립,이종석,황규왕,염윤기 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Background & Objective: Upon UV irradiation of skin, matrix metalloproteinases are induced and then degrade collagen and elastin which provide the structural integrity of skin. The purpose of this study is to investigate the inhibition of elastase by SCH-T3 extracted from marine plants. Method: From gathered seaweeds, which are Laminariacea and Alariaceae in Phaeophyta, In Korea water, 21 materials were extracted. To determine the optimal condition for the extraction of SCH-T3, the yields of SCH-T3 was measured compared at various solvents, concentrations, and extraction time. Finally, the inhibitory effect of SCH-T3 on elastase was measured under various conentrations of SCH-T3. Results: Among 21 extracts, SCH-T3 had excellent elastase inhibitory effect. The yields of SCH-T3 was highest with the use of 60% and 70% ethanol as sovents, at 12 hours extraction, and with increasing temperature. Conclusion: These results suggest that SCH-T3, extracted from marine plants, has an inhibitory effect on elastase in vitro and may be used in prevention and treatment of photoaging in the future.

Streptomyces exfoliatus가 생성되는 mutanase에 의한 인공치태 억제 작용

오종석,정진,송도원,양규호 大韓小兒齒科學會 1997 大韓小兒齒科學會誌 Vol.24 No.2

Streptomyces exfoliatus 의 mutan 분해 능력에 영향을 미치는 인자에 대해 알아보고자 blue mutan을 이용한 실험을 실시하여 다음과 같은 결과를 얻었다. Blue mutan이 들어있는 배지에서 Streptomyces exfoliatus에 의한 투명대를 봄으로써 mutanase의 분비를 확인할 수 있었다. Blue mutan이 들어있는 여러 종류의 배지 중에서 minimal essential agar 상에서 Streptomyces exfoliatus가 투명대를 형성하였다. Minimal essential broth의 pH가 7.0일때 pH가 5.5나 8.5일 때보다 blue mutan이 잘 분해되었으며, 배양 온도가 37。c때 32。나 42。때보다 잘 분해되었다. (P<0.05). Minimal essential broth에서의 blue mutan의 분해는 CaCl₂농도가 증가할 수록 (p<0.05), KCI 농도가 10mM일때 증가하였으나, MgCl₂ 농도는 0.1M에서 6.4mM의 범위에서 비슷하였다. Streptomyces exfoliatus배양 상청액을 0.5% yeast extract와 10% sucrose를 첨가한 2배의 BHI broth에 가한 경우 교정용 wire상에서의 Streptococcus mutans에 의한 인공치태 형성이 유의성있게 억제되었다 (p<0.05). 이상의 결과를 종합하면 Streptomyces exfoliatus에서 생성되는 배양 상청액내의 mutanase가 인공치태 형성에 억제 작용이 있다는 것을 확인할 수 있었다. The main component of dental plaque is the mutan containing the a-1,3 bond. The following results were obtained by using a blue mutan to assess the factors affecting the mutandigesting activity of Streptomyces exfloiatus isolated from soil. A clear zone was produced by mutanase-producing Streptomyces exfoliatus on the minimal essential agar containing blue mutan. Streptomyces exfoliatus digested more blue mutan in the minimal essential broth at pH 7.0 than at pH5.5 or 8.5 Streptomyces digested more blue mutan at 37。c than at 32。c or 42。c (p<0.05). When the concentration of CaCl₂was increased in the minimal essential broth, the digestion of the blue mutan was increased (p<0.0.). The optimal concentration of KCI was 10mM to digest blue mutan, but a similar amount of blue mutan was digested at the range of 0.1mM to 6.4mM of MgCl₂. When the culture supernatant of Streptomyces exfoliatus was mixed with 2X brain heart infusion broth containing 0.5% yeast extract and 10% sucrose, less artificial plaque was formed by Streptococcus mutans on the orthodontic wire (p<0.05). These results indicated that the secretion of mutanase was identified in culture supernatant of mutan-digesting Streptomyces exfoliatus, suppressing the formation of artificial plaque by Streptococcus mutans.

백색부후균 전처리에 의한 목재 칩의 Biopulping

강규영,김형진,양봉숙,조병묵,오정수 동국대학교 생명자원과학대학 연습림 2000 연습림논문집 Vol.- No.6

Pulping process is to mainly obtain lignocellulosic fibers for papermaking from wood resources. Three types of pulping method have been basically employed, such as chemical pulping, mechanical pulping, and their combination methods. Biopulping technique has been mainly applied in mechanical pulping by pretreatment of white-rot fungi into the ultrastructure of wood cell wall. The main purpose of biopulping is to improve the pulp qualities, paper properties, and to reduce the energy costs and environmental impact, compared to traditional pulping systems. Nowadays, biopulping is frequently tried in chemical pulping operation for environmental concept, fiber modification, improvement of physical properties of paper and economic savings. The pretreatment of white-rot fungi prior to chemical pulping could affect the swelling and softening of wood cell walls by the behaviors of modification and depolymerization of ligin compounds. This study was carried out to investigate the effectiveness of biological treatment to wood chips by white-rot fungus and its structural change of lignocellulosic fiber via kraft pulping. A rotary bioreactor was applied for pretreatment of biological action. The treatment of white-rot fungus was shown in weight loss of chips. After bio-kraft pulping, kappa number and total yield of pulps were measured, and reduced. Freeness(CSF) values were also decreased. Whereas brightness values were increased, compared to untreated control. The physical strength of handsheets was shown a slight increase in tensile, tear, burst, and folding endurance, with bio-treatments.

상악 정중부에 역위 매복된 과잉치의 악골 내 이동

이석우,이제호,김성오,최형준,손홍규,최병재 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.4

과잉치는 상악 정중부에서 주로 발견되며,과잉치의 발생 기전은 유전적 또는 환경적 원인에 의하여 치배 형성기 동안에 정상 치판의 비정상적 증식에 의한 것으로 보고되었다. 과잉치로 인한 합병증으로는 인접 영구치의 맹출 장애와 전위,회전,정중이개,비강으로의 맹출,낭종 형성 등 이 있다. 과잉치를 외과적으로 발거하기 위한 시기에 대해서는 논란이 되고 있다. 인접치의 배열과 맹출 장애,전위를 야기할 수 있기 때문에 조기에 발거하자는 견해와 어린이의 행동 조절 및 인접 영구치의 치근 형성 정도를 고려하여 8~l0세 이후에 발거 하자는 견해가 있다. 후자와 같이 외과적 발거 시기를 연기할 경우,역위 및 수평 매복된 과잉치는 악골 내에서 비강 또는 경구개,소구치 부위 등으로 이동할 수 있으며,과잉치의 이동과 전상악골의 수직고경 증가로 인하여 외과적 발거 시 난이도가 높아질 수 있다. 따라서,역위 및 수평 매복된 과잉치의 외과적 발거 시기를 결정하기 위하여 연기하는 경우에 과잉치의 악골 내 이동을고려해야한다. 이 증례는 상악 정중부에 역위 매복된 과잉치가 있어서 내원한 6~7세의 어린이에서 과잉치의 외과적 발거 시기를 결정하기 위하여 주기적인 방사선 학적 검사로 관찰하던 중에 2~3년 후 과잉치 위치가 악골 내에서 초진 시 위치로부터 이동된 경우로 이를 보고하는 바이다. Supernumerary teeth are frequently found in the anterior portion of the maxilla and develop as a result of abnormal proliferation of the dental lamina during tooth germ formation, caused by genetic or environmental factors. They may result in various complications, such as eruption interference, displacement, rotation of adjacent teeth, diastema, eruption into the nasal cavity, and development of dentigerous cyst. The optimal time for surgical extraction of supernumerary teeth has been a controversial issue. Someone prefer early surgical extraction because supernumerary teeth can cause eruption interference and displacement of adjacent teeth, eventually altering occlusion. Others prefer to delay surgical extraction until 8~10 years of age in consideration of root maturation of the adjacent teeth and also patient's behavior. When surgical extraction of supernumerary teeth is postponed, there is possibility that impacted supernumerary teeth in the inverted or horizontal position move toward the nasal cavity, hard palate, or premolar area. When such intraosseous tooth migration is combined with the vertical growth of the maxilla. surgical approach becomes even harder. Therefore, possibility of intraosseous tooth migration should be considered as an important factor when deciding appropriate time for surgical extraction. We are presenting cases of mesiodens which showed intraosseous migration during 2~3 years of follow-up period since the first diagnosis had been made at the 6~7 years of age.