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Jia You,Lirong Huang,Jialin Zhuang,Zongchun Mou 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.1
A multiplex real-time PCR method fordiscriminating deer and common domestic species,including cattle, goat, horse, donkey, pig, and chicken wasdeveloped. Species-specific primer pairs were designedand used to produce different size DNA fragments withdiverse melting temperature (Tm) values. The specificityand sensitivity of these primer pairs were separatelyconfirmed using simplex real-time PCR analysis. Multiplexreal-time PCR analysis was performed using combinedprimers, yielding distinct melting curve profiles for eachspecies. The sensitivity limit of the multiplex PCR methodwas evaluated. Trace DNA of other species in deer DNAcould be identified. Common deer products, includingblood, meat, and antler were tested using this multiplexPCR method and different species of deer and domesticanimals were identified. The rapid multiplex real-time PCRassay described herein is a specific, sensitive, and reliablemethod for high-throughput authentication of deer anddomestic animal products.
A Coordinated Ciphertext Policy Attribute-based PHR Access Control with User Accountability
( Guofeng Lin ),( Lirong You ),( Bing Hu ),( Hanshu Hong ),( Zhixin Sun ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.4
The personal health record (PHR) system is a promising application that provides precise information and customized services for health care. To flexibly protect sensitive data, attribute-based encryption has been widely applied for PHR access control. However, escrow, exposure and abuse of private keys still hinder its practical application in the PHR system. In this paper, we propose a coordinated ciphertext policy attribute-based access control with user accountability (CCP-ABAC-UA) for the PHR system. Its coordinated mechanism not only effectively prevents the escrow and exposure of private keys but also accurately detects whether key abuse is taking place and identifies the traitor. We claim that CCP-ABAC-UA is a user-side lightweight scheme. Especially for PHR receivers, no bilinear pairing computation is needed to access health records, so the practical mobile PHR system can be realized. By introducing a novel provably secure construction, we prove that it is secure against selectively chosen plaintext attacks. The analysis indicates that CCP-ABAC-UA achieves better performance in terms of security and user-side computational efficiency for a PHR system.