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      • KCI등재

        Phenazine-1-carboxamide, an Extrolite Produced by Pseudomonas aeruginosa Strain (CGK-KS-1) Isolated from Ladakh and India, and its Evaluation Against Various Xanthomonas spp.

        ( K. Sirisha ),( C. Ganesh Kumar ),( Kallaganti Venkata Siva Ramakrishna ),( Shravan Kumar Gunda ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 한국미생물·생명공학회지 Vol.45 No.3

        In the enduring investigation of the bioactive microbes, Pseudomonas aeruginosa strain (referred to as CGK-KS-1 (ICTB-315)), isolated from Chumathang hot spring, Ladakh, and India, was identified to possess a major bioactive fraction with antimicrobial and anti-biofilm properties. This bioactive metabolite was purified through bioactivity-guided fractionation. The chemical structure of this major compound was elucidated as phenazine-1-carboxamide (PCN) based on <sup>1</sup>H and <sup>13</sup>C NMR, FT-IR, EI-HR-MS and 2D NMR spectroscopic techniques. In the current study, PCN exhibited antimicrobial activity with MIC values ranging between 1.9-3.9 μg/ml against various test human pathogens and Xanthomonas spp. PCN showed the antibiofilm property with the IC<sub>50</sub> values ranging from 17.04 to 60.7 μM against different test pathogens. The in silico docking studies showed PCN strongly interacted with various proteins of different Xanthomonas spp. with high binding energies. We report herein for the first time the anti-biofilm property and the docking studies of PCN. The extrolite from P. aeruginosa strain CGK-KS-1 showed promising bioactivities and may be considered as a potential candidate for application in various biocontrol strategies.

      • Low Frequency AC Transmission System

        G. Sirisha Kumari,K.Veerendranath 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.5

        Presently, High Voltage AC transmission and High Voltage DC transmission systems are well established technologies for transmission of power. A new Low Frequency AC transmission system employs transmission at an intermediate frequency and thus, establishes itself in between these two alternatives. Low Frequency AC transmission system can transmit bulk power over long distance with low investment cost. This system is based on generation at low frequency and AC to AC conversion from nominal to low frequency using power electronic devices. This technology is more reliable and provides a cost effective solution for power transmission. This paper presents the feasibility of applying low frequency AC transmission technology to interface the wind farm to grid, which is a major issue. The wind power plant collection is DC based and connects transmission line with 12-pulse converter. This system is interfaced with main power grid with cycloconverter. Low Frequency AC transmission system is implemented with a suitable controller. The system design and control strategies are discussed. System performances are verified using MATLAB/SIMULINK.

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