발효조를 이용한 Monascus anka의 적색소와 황색소의 생산

강성국,임종환,정순택,김선재 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

Monascus anka를 이용하여 천연식용색소를 대량생산하기 위한 방법을 개발하기 위하여 발효조를 이용하여 적색소와 황색소의 색소생산의 최적 배양 조건과 생산된 색소의 색깔 특성을 조사하였다. Monascus anka 적색소와 황색소를 생산하였는데, 이들 색소는 균체내색소와 균체외 색소로 구분되었다. 균체의 적색소(ERP)는 494nm에서, 균체외 황색소(EYP)는 380nm에서, 균체내 적색소(IRP)는 506nm에서 그리고 균체내 황색소(IYP)는 388nm에서 최대 흡광도를 나타냈다. 적색소와 황색소 생산, 색조의 특성 및 균체증식력을 배양 온도, pH, 쌀가루 농도, peptone 농도, magnesium sulfate 농도, 통기량 및 교반속도에 대하여 조사한 결과 적색소 생산력은 30℃, 초기 pH 6.0, 쌀가루 농도 3~5%, peptone 농도 0.05%, magnesium sulfate 농도 0.25%, 통기량 0.1vvm 교반속도 300rpm의 조건에서 최대치를 보였으며, 이때 ERP, EYP, IRP 및 IYP는 각각 A_494nm 0.84, A_380 nm 0.71, A_506 nm 1.18, A_388 nm 1.10, L값은 각각 29.40, 6.44, 34,98, 8.94, a값은 각각 5.76, -1.08, 11.83, -1.55, b값은 각각 18-19, 3.41 27.26, 6.16 그리고 균체량은 7.4 g/l을 보였다. 또한 황색소 생산력은 온도의 경우 적색소 생산을 위한 최적온도 보다 높은 35℃ 부근에서 우수하였으며 초기 pH와 쌀가루 농도는 적색소 생산을 위한 최적조건과 같았고, 질소원과 무기염의 농도가 높을수록 그리고 통기량이 많을수록 황색소 생산력이 우수하였다. 적색소와 황색소 생산을 위한 발효조의 교반속도는 100~300 rpm이 적합하였다. Production of Red and Yellow Pigments from Monascus anda in a Jar Fermenter, Seong-Gook Kang, Jong-Whan Rhim, Soon-Teck Jung^* and Sun-Jae Kim. Department of Food Engineering. Mokpo National University Chonnam, 534-729, Korea - In order to develop the method for mass production of natural food colorant from Monascus anka, optimum cultivation conditions for producing red and yellow pigments by cultivating the mold in a jar fermenter and their color characteristics were investigated. The mold produced red and yellow pigments both intracellularly and extracellularly. These pigments showed unique light absorption characteristics with maximum absorption of 494, 380, 506, and 388 nm for extracellular red pigment (ERP), extracellular yellow pigment (EYP), intracellular red pigment (IRP), and intracellular yellow pigment (IYP), respectively. Optimum conditions for producing red pigments were found to be temperature 30℃, initial pH 6.0, rice powder 3~5%, peptone 0.05%, magnesium sulfate 0.25%, aeration rate 0.1vvm. Optimum temperature for producing yellow pigments was around 35℃ which is higher than that of producing red pigments. The initial pH rice powder concentration for producing yellow pigments were the same as those of producing red pigments. The higher concentration of nitrogen source and inorganic salt, aeration rate, the more the yellow pigments were produced. Them optimum agitation speed was 100~300 rpm for pigment production.

윤활유 종류에 따른 주축 열변위의 특성 평가

강순준,이갑조,김종관 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.2

High speed precision machines have been introduced to the CNC industry in order to improve productivity, shorten the appointed date of delivery and reduce the prime cost. High speed machines have more functions then general maches, and they were proved in performance. The production and sales of the high speed machines have been increased not only in domestic market but also all over the world. Accordingly, machines are faster, there are lots of problems to be solved. One of the most difficult problems is the thermal displacement on the main spindle due to generated heat while the spindle is rotated in high speed. Since the thermal displacement directly effects the quality of the machined parts, utmost efforts to minimize the thermal displacement have to be given from the beginning of designing machines. In practice, variety of methods are attempted and practiced to minimize the thermal displacement such as design of symmetrical lime, adoption of high speed bearings. application of compensation system using non-contact sensor and use of forced circulating lubrication system with oil cooler. Even if these variable methods have been practically used in the industrial field, generated heat has not been perfectly prevented. Hence, in this pager, the characteristics of thermal displacement were investigated when several kinds of oil were tested for a high speed machine with forced circulating lubrication system within the same atmosphere and under the same conditions.

우심방을 침범한 거대 비호지킨씨 림프종 1예

강상범,진승원,이은경,박용현,최용호,김용주,박준철,김재형,홍순조,최규보,권종범,원용순,박건,이은희 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

고등학교 계열별 학업 성취도에 관한 연구

강현중,박종순 瑞逸大學 1999 論文集 Vol.17 No.-

근래들어 2년제 대학(전문대학)의 입학에 실업계 출신 학생들의 정원 비율이 높아지고 있다. 특별전형의 방법이 다양해지고 정원도 늘면서 전체 입학생 중 실업계 고교 출신자들의 비율이 늘고 있는 것이다. 전문대학이 중견 전문기술인의 양성을 목표로 하고 있기 때문에 이는 바람직한 방향으로 볼 수도 있으나 다른 한편으로 실업계 출신 학생들의 학업 성취도가 일반계 고교 출신 학생들보다 낮다고 생각하며 우려하는 시각이 있는 것도 사실이다. 이러한 관점에서 실제로 실업계 고교 출신 학생들과 일반계 고교 출신 학생들의 학업 성취도에 차이가 있는 지를 알아보기 위해 본교의 재학생을 대상으로 간단한 실험적 분석을 하였다.

SEM 측정법에 의한 초정밀 표면가공 특성연구

강순준,오상록,이갑조,김종관 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

The purpose of this paper is to look at the characteristics of surface finishing which is one of the form accuracies and to obtain the fundamental technical data from the process of machining with diamond tool through experiment and theoretical analysis. The experiments were conducted with domestic made ultra-precision machine and MCD·PCD tool, with aluminum alloyed material and brass being used for the work pieces. The goal of the size accuracy was set to 100nm. The most suitable tool nose radius and machining conditions were selected, and the variations of the surface roughness were observed using SEM method while machining the distance of up to 500km. These data were evaluated and they examined the variation of the machined surfaces while cutting up to 500km of machining distance. At the same time, the state for the wear of diamond tool nose was analyzed and carefully examined through the newest measuring device. Additionally, the characteristics of ultra-precision machining technology were studied through visual analysis.

인지질말과 콜레스테롤을 포함한 인지질막의 수용액에서의 산화반응 속도에 관한 연구

康順姬,朴鐘倫,金健,盧貞銀 梨花女子大學校 韓國生活科學硏究院 1988 韓國生活科學硏究院 論叢 Vol.42 No.-

생체막의 중요 성분중의 하나인 지질의 산화반응에 대한 연구로 모델 생체막인 인지질 이중층막을 제조한 후 pH 7.5 완충용액에서 KMnO_4로 산화시킬 때의 속도는 근사 일차 속도식으로 나타났다. PC 이중층막의 산화반응 속도 상수를 25℃에서 40℃ 온도 구간에서 얻어낸 결과 급격한 속도 상승 증가 현상이 PC 이중층막의 상전이 온도 구간에서 관측되었다. 그리고 PC에 콜레스테롤 10mol% 포함한 혼합 이중층막의 산화 속도 상수는 PC 이중층막의 산화속도 상수보다 모든 온도구간에서 크게 나타났다. The kinetics of oxidation of phosphatidyl choline(PC)and PC-cholesterol-reconstituted vesicle on water has been studied at 20°, 25°, 30°, 35°, and 40℃. The oxidation was initiated by KMnO_4 in pH 7.5 Tris-buffer aqueous solution and the product was measured by UV absorption at 230nm. The kinetics of oxidation of PC-vesicle and PC-cholesterol-reconstituted vesicle by KMnO_4 was pseudo-first order reaction. The oxidationof PC-cholesterol reconstituted vesicle was faster than that of PC vesicle. The highest difference of rate constants of PC vesicle was observed in the range of the phase transition temperature (35∼40℃) of PC-vesicle.

초정밀 절삭가공에서 표면거칠기 특성 평가

강순준,김종관 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.1

In this study. experiments were conducted with an ultra-precision machine. developed in domestic. to find the characteristics and the most suitable cutting conditions of ultra-precision machining. To maximize the performance of the machine, the machine was installed in a room that is protected from vibration and is maintained constant temperature and constant humidity. Selected work pieces are an aluminum-alloyed material, which has excellent corrosion resistance and has loll deformation. The used tool is synthetic poly crystal diamond, which has excellent abrasion resistance and has low affinity, Four types of tool nose radius were used such as 0. 0.1, 0.2 and 0.4mm. machining is performed with cutting speed of 500, 800 and 1000m/min. feed rate of 0.005, 0.008, 0.01mm/rev. and cutting depth of 0.0005, 0.0025 and 0.005mm respectively which can generally be used in the field as a cutting condition. As a method of evaluation, surface roughness was measured for each cutting condition. and reciprocal characteristics are computed for each tool nose radius. cutting speed, feed rate and cutting depth. As a result. the most suitable cutting condition and characteristics of ultra-precision machining were identified which can usefully be applied in the industrial field.

새로운 반복이완법에 의한 선소의 정합

강창순,배종갑 동의공업대학 2000 論文集 Vol.26 No.1

The line segment matching problem in stereo vision is solved by a new iteration scheme. A match function which directly reflects the requirements of the epipolar and disparity constraints is proposed for line segment matching. The information contained in the match function can be used to determine line segment correspondences indirectly. After a match network is established according to the match function values, a new iteration algorithm is employed to tune the strengths of the match links in the match network so that the match network can converge to a stable state. No explicit compatibility coefficient need be defined for computing the support function values in the iterations, so that computation time can be saved, compared with conventional relation matching techniques. The inherent anti-symmetric characteristic of relaxation matching for the correspondence problem is also avoided naturally. The experimental results show that the proposed iteration scheme is effective and suitable for various stereo images.

음향궤환제거에 의한 보청기 성능향상

강창순,배종갑 동의공업대학 2001 論文集 Vol.27 No.1

This paper presents a new method of acoustic feedback cancellation in hearing aids which is based on the orthogonality principle. This method is less affected by ambient signals in steady state, therefore the adaptive filter estimates the feedback path well. The improved performance of this method is confirmed by computer simulation.

外筒과 윗板이 回轉하는 二重圓筒內에서의 定常層流 流動解析

姜昌壽,權純範,李鍾鵬 경북대학교 공과대학 1981 工大硏究誌 Vol.10 No.-

In this paper, steady laminar flow in a tank of double cylinder with rotating outer cylinder and upper disc was analyzed numerically. For simplicity, the stream function and vorticity have been introduced into the Navier-Stokes and the continuity equations. The transformed governing equations were converted into the fimite difference equations which were solved numerically by ADI (Alternating direction implicit) and SUR (Successive under relaxation) method.