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( Haram Kong ),( Do-won Jeong ),( Namwon Kim ),( Sugyeong Lee ),( Sooyoung Sul ),( Jong-hoon Lee ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.4
We evaluated the antibiotic susceptibilities, hemolytic activities, and technological properties of 36 Staphylococcus xylosus strains and 49 S. pseudoxylosus strains predominantly isolated from fermented soybean foods from Korea. Most of the strains were sensitive to chloramphenicol, erythromycin, gentamycin, kanamycin, lincomycin, oxacillin, tetracycline, and trimethoprim. However, 23 strains exhibited potential phenotypic acquired resistance to erythromycin, lincomycin, and tetracycline. Based on breakpoint values for staphylococci from the Clinical and Laboratory Standards Institute, >30% of the isolates were resistant to ampicillin and penicillin G, but the population distributions in minimum inhibitory concentration tests were clearly different from those expected for acquired resistance. None of the strains exhibited clear α- or β-hemolytic activity. S. xylosus and S. pseudoxylosus exhibited salt tolerance on agar medium containing 20% and 22% (w/v) NaCl, respectively. S. xylosus and S. pseudoxylosus strains possessed protease and lipase activities, which were affected by the NaCl concentration. Protease activity of S. pseudoxylosus was strain-specific, but lipase activity might be a characteristic of both species. This study confirms the potential of both species for use in high-salt soybean fermentation, but the safety and technological properties of strains must be determined to select suitable starter candidates.
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메주 유래 Enterococcus faecium 균주의 기능적 특성 및 안전성
오영민 ( Yeongmin Oh ),공하람 ( Haram Kong ),정도원 ( Do-won Jeong ),이종훈 ( Jong-hoon Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 한국미생물·생명공학회지 Vol.49 No.2
γ-Aminobutyric acid 생성에 관여하는 glutamate decarboxylase 유전자 gadA/B를 보유한 메주 유래 Enterococcus faecium 88균주를 대상으로 기능적 특성 및 안전성 평가를 진행하여 메주 유래 E. faecium의 발효식품용 종균으로써의 활용 가능성을 검토하였다. 6% NaCl 농도(w/v)에서 모든 균주의 생장 및 산 생성이 확인되었다. 7% NaCl 농도에서는 21균주(24%)가 낮은 생장을 나타냈으며, 72균주(82%)가 약한 산 생성 활성을 나타냈고, 16균주(18%)는 산생성을 나타내지 않았다. 4% NaCl 농도에서는 모든 균주가 단백질 분해 활성을 나타냈지만, 5% NaCl 농도에서 86균주(98%)가 약한 활성을 나타냈고, 1균주(1%)는 활성을 나타내지 않았다. 지방 분해 활성은 모든 균주에서 나타나지 않았다. 모든 균주가 7종의 항생제(ampicillin, chloramphenicol, ciprofloxacin, gentamicin, penicillin G, tetracycline, vancomycin)에 대해 획득형 항생제 내성을 나타내지 않았다. Enterococcus 심내막염 항원 유전자 efaA 및 tyramine 생성에 관여하는 tyrosine decarboxylase 유전자 tdc가 메주유래 88균주에서 발견되었지만, 사람 기원 E. faecium 균주가 특이적으로 보유하고 있는 Enterococcus surface protein 유전자 esp는 발견되지 않았다. In this study, we assessed the technological characteristics and safety of 88 Enterococcus faecium strains isolated from meju; the strains possess the glutamate decarboxylase gene gadA/B involved in γ-aminobutyric acid production. The study was conducted to evaluate the possibility of introducing E. faecium meju isolates as food fermentation starters. We observed that a NaCl concentration of 6% (w/v) facilitated the growth and acid production of all strains. At a NaCl concentration of 7%, 21 strains (24%) exhibited a low growth rate, 72 strains (82%) a weak acid production, and 16 strains (18%) showed no acid production. All strains exhibited protease activity at a NaCl concentration of 4%. At a NaCl concentration of 5%, 86 strains exhibited weak activity, and one strain showed no protease activity. We could not detect any lipase activity in the investigated strains. None of the strains exhibited an acquired antibiotic resistance to the seven antibiotics tested in the present study, namely ampicillin, chloramphenicol, ciprofloxacin, gentamicin, penicillin G, tetracycline, and vancomycin. We could identify the Enterococcus endocarditis antigen gene efaA and the tyrosine decarboxylase gene tdc contributing to tyramine production, in 88 meju isolates. We could not detect the Enterococcus surface protein gene esp, which is specifically possessed by human-originated E. faecium strains, in any of the 88 strains tested in the study.
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( Mihyun Jang ),( Do-won Jeong ),( Ganghun Heo ),( Haram Kong ),( Cheong-tae Kim ),( Jong-hoon Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.3
Strains of four Bacillus spp. were respectively inoculated into sterilized soybeans and the free amino acid profiles of the resulting cultures were analyzed to discern their metabolic traits. After 30 days of culture, B. licheniformis showed the highest production of serine, threonine, and glutamic acid; B. subtilis exhibited the highest production of alanine, asparagine, glycine, leucine, proline, tryptophan, and lysine. B. velezensis increased the γ-aminobutyric acid (GABA) concentration to >200% of that in the control samples. B. sonorensis produced a somewhat similar amino acid profile with B. licheniformis. Comparative genomic analysis of the four Bacillus strains and the genetic profiles of the produced free amino acids revealed that genes involved in glutamate and arginine metabolism were not common to the four strains. The genes gadA/B (encoding a glutamate decarboxylase), rocE (amino acid permease), and puuD (γ-glutamyl-γ-aminobutyrate hydrolase) determined GABA production, and their presence was species-specific. Taken together, B. licheniformis and B. velezensis were respectively shown to have high potential to increase concentrations of glutamic acid and GABA, while B. subtilis has the ability to increase essential amino acid concentrations in fermented soybean foods.
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