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( Yongbae Seo ),( Seongseok Choi ),( Jongkyu Lee ),( Nanhee Kim ),( Mijin Choi ),( Jongmyoung Kim ),( Taehyug Jeong ),( Soowan Nam ),( Hankyu Lim ),( Gundo Kim ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.11
A phytoene synthase gene, crtB, was isolated from Kocuria gwangalliensis. The crtB with 1,092 bp full-length has a coding sequence of 948 bp and encodes a 316-amino-acids protein. The deduced amino acid sequence showed a 70.9% identity with a putative phytoene synthase from K. rhizophila. An expression plasmid, pCcrtB, containing the crtB gene was constructed, and E. coli cells containing this plasmid produced the recombinant protein of approximately 34kDa , corresponding to the molecular mass of phytoene synthase. Biosynthesis of lycopene was confirmed when the plasmid pCcrtB was co-transformed into E. coli containing pRScrtEI carrying the crtE and crtI genes encoding lycopene biosynthetic pathway enzymes. The results obtained from this study will provide a base of knowledge about the phytoene synthase of K. gwangalliensis and can be applied to the production of carotenoids in a non-carotenoidproducing host.
박철수,설한신,김건도,박영하,Park, Cheolsoo,Seol, Hanshin,Kim, Gundo,Park, Youngha 한국음향학회 2013 韓國音響學會誌 Vol.32 No.3
본 논문에서는 캐비테이션 터널에서의 소음계측용 청음기 배열 설계를 위한 최적화 기법을 제안하였다. 제안된 최적설계 기법은 배열 설계인자 및 목적함수 정의 그리고 최적화 알고리즘 적용 등의 내용으로 구성되어 있다. 설계인자 정의는 원형배열, 나선배열, 다중나선배열을 대상으로 하였다. 목적함수는 주엽의 빔폭과 최대 부엽 크기를 동시에 고려할 수 있도록 정의하였다. 최적화 알고리즘으로는 광역 최적화 기법의 일종인 VFSR 기법을 적용하였다. 최적 설계기법을 각 배열에 적용 후 도출된 최적 배열을 대상으로 최대 부엽크기 및 주엽의 빔폭을 분석하였다. 끝으로 캐비테이션 터널 내부의 다중반사를 고려한 빔형성 결과 평가를 통해 본 기법의 유용성을 확인하였다. This paper proposes a hydrophone array design optimization technique for cavitation tunnel noise measurements. The optimization technique comprises of design parameters, an objective function and an optimization algorithm. The design parameters are defined for circular, spiral and multi-spiral arrays. The objective function is defined so as to consider the mainlobe beamwidth and the maximum sidelobe level simultaneously. A global optimization scheme is applied to the array design using very fast simulated reannealing (VFSR). After applying the optimization technique to arrays respectively, the peak sidelobe level and the mainlobe beamwidth of optimum arrays are analyzed. Finally the array patterns considering multiple reflections in the cavitation tunnel are evaluated to validate the proposed method.
( Jung-hee Kwon ),( Keun Soo Ahn ),( Yun Suk Yu ),( Jin Young Park ),( Gundo Kim ),( Seung Whan Kim ),( Hongdu Gu ),( Hee Jung Wang ),( Jae Won Joh ),( Koo Jeong Kang ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims: In the previous array-based analysis of gene expression and DNA methylation associated with recurrence of HCC, up-and down-regulating genes affecting on the HCC recurrence were extracted, that was reported already by our cooperative group. Methods: In this study, we validated the down-expressed and hyper- methylated genes (MRPL4, PCDHB11, SN2, CYGB, HSD17B6) and an overexpressed and hypo-methylated gene(NMB) in independent cohort(n=90) using HCC tissues and paired normal liver tissues collected from multicenter in Korea. We measured gene expression of the six genes using real time RT-PCR from the normal and HCC tissues, analyzed correlation of prognosis and gene expression between tumor and non-tumor tissues using student t-test and their prognostic significance using Cox regression analysis. Results: Out of six genes, CYGB and PCDHB11 were little expressed in both tumor and non-tumor, which is not consistent with our previous result. In apart, GPSN2 and MRPL4 were over expressed in tumor tissues in comparison to normal.(p<0.0001) However NMB was overexpressed in tumor tissue than in non-tumor tissue.(p=0.0002) as same as the previous resuIt. HSD17B6 was down-regulated, but is not significant (p=0.5980), in tumor tissues compared to the non-tumor tissues. Regarding three significant genes(GPSN2, MRPL4, NMB) in t-test, the patients who have low expression of GPSN2 has shown higher recurrence rate(p=0.0458), the patients who have higher expression group of MRPL4 shows higher recurrence rate.(P=0.0385). The high expression group of NMB shows higher recurrence rate (P=0.04417) and shorter disease free survival rate(P=0.168) In the univariate cox-regression analysis of significant genes and clinical parameters, GPSN2, MRPL4 and NMB were significant with Edmonson-Steiner grade, HBV positivity, high AFP level, tumor state and vascular invasion. In multivariate analysis, only NMB was an independent prognostic factor.(p=0.031) Conclusions: In our study of gene expression and its correlation with clinical markers on the basis of microarray thereafter with quantitative assay of the specific markers, validation is very important for the next step validation with high volume data set. A gene that we validated may have significant role in the prognosis of HCC.
AROS Is a Significant Biomarker for Tumor Aggressiveness in Non-cirrhotic Hepatocellular Carcinoma
Kwon, Jung-Hee,Ahn, Keun Soo,Moon, Young Ho,Park, Jin Young,Wang, Hee Jung,Choi, Kwan Yong,Kim, Gundo,Joh, Jae Won,Lee, Kyeong Geun,Kang, Koo Jeong The Korean Academy of Medical Sciences 2015 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.30 No.9
<P>Despite a low risk of liver failure and preserved liver function, non-cirrhotic hepatocellular carcinoma (HCC) has a poor prognosis. In the current study, we evaluated an active regulator of SIRT1 (AROS) as a prognostic biomarker in non-cirrhotic HCC. mRNA levels of AROS were measured in tumor and non-tumor tissues obtained from 283 non-cirrhotic HCC patients. AROS expression was exclusively up-regulated in recurrent tissues from the non-cirrhotic HCC patients (<I>P</I> = 0.015) and also in tumor tissues irrespective of tumor stage (<I>P</I> < 0.001) or BCLC stage (<I>P</I> < 0.001). High mRNA levels of AROS were statistically significantly associated with tumor stage (<I>P</I> < 0.001), BCLC stage (<I>P</I> = 0.007), alpha fetoprotein (AFP) level (<I>P</I> = 0.013), microvascular invasion (<I>P</I> = 0.001), tumor size (<I>P</I> = 0.036), and portal vein invasion (<I>P</I> = 0.005). Kaplan-Meir curve analysis demonstrated that HCC patients with higher AROS levels had shorter disease-free survival (DFS) in both the short-term (<I>P</I> < 0.001) and long-term (<I>P</I> = 0.005) compared to those with low AROS. Cox regression analysis demonstrated that AROS is a significant predictor for DFS along with large tumor size, tumor multiplicity, vascular invasion, and poor tumor differentiation, which are the known prognostic factors. In conclusion, AROS is a significant biomarker for tumor aggressiveness in non-cirrhotic hepatocellular carcinoma.</P>