Similarity measurement of the geometry variation sequence of intermediate process model

Chunlei Li,Liang Li,Xiaoye Wang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.7

The reuse of machining process, by which the process for a new mechanical part is determined by referencing to the existing and matured processes, is an effective way of improving manufacturing and supporting innovation. To conduct the effective reuse, it is necessary to express and retrieve a specific process. A kernel technique of the expression and the retrieval is to measure the similarity of part’s geometry variation during the machining. To address this problem, a general framework of measuring the similarity between parts is proposed in this work. The geometry variation sequence of intermediate process model was established, and then a method to measure its similarity was invented. Two case studies are rendered and the result reveals that the proposed method is effective and can provide the support for the process retrieval and reuse in industry.

Ionic liquid gating control of magnetic anisotropy in Ni0.81Fe0.19 thin films

Chunlei Li,Shishun Zhao,Ziyao Zhou,Bin Peng,Zhongqiang Hu,Ming Liu 한국물리학회 2020 Current Applied Physics Vol.20 No.7

Voltage control magnetism is one of the most energy efficient pathway towards magnetoelectric (ME) device. Ionic liquid gating (ILG) method has already shown impressive manipulation power at the IL/electrode interface to influence the structure, orbital as well as spin of the electrode materials. As key material in anisotropy magnetoresistance sensor and spin valve heterostructure, the permalloy Ni0.81Fe0.19 was utilized as the electrode to investigate the ILG induced magnetic anisotropy change. In this work, we realized magnetic anisotropy control in Au/[DEME]+[TFSI]-/Ni0.81Fe0.19 (2.5 nm)/Ta heterostructure via ILG caused electrostatic doping. This is evidenced in situ reversible ferromagnetic field (Hr) shift with electron spin resonance (ESR) spectrometer. Aiming at the question whether the charge accumulation at the ionic liquid interface is the main control mechanism at low voltage, we carefully tested the relationship between the change of resonance field and the amount of surface charge. It was found that these two had a good linear relationship between −1 V and +1 V. Defining the linear parameter as A whose value is 28.7 mT m2/Col. Unlike previously reported chemical regulation of Co, this article used ionic liquids to physically regulate NiFe, which has not been studied in the previous ionic liquid regulation. And NiFe has a narrower resonance line width for easy reference to microwave devices. In addition, It also has a stronger ferromagnetic signal than Co, which can be more easily detected as a sensor device. Therefore, this system is more promising. The ILG control NiFe may lead to a new kind of magnetoelectric sensor devices and path a new way to low energy consumption spintronics.

Properties of the Cu₂ZnSn(S<SUB>x</SUB>Se<SUB>1-x</SUB>)₄ thin films and solar cells by sulfurization of Cu-Zn-Sn-Se precursors

Zhaohui LI,Wenjie LI,Guanming CHENG,Ye FENG,Chunlei YANG,Xudong XIAO 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling

Liu Chunlei,Li Peng,Ao Xiang,Lian Zhengnan,Liu Jie,Li Chenglong,Huang Minjun,Wang Liang,Zhang Zhongmin 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Ligamentum flavum hypertrophy (LFH) is a major cause of lumbar spinal canal stenosis (LSCS). The pathomechanisms for LFH have not been fully elucidated. Isobaric tags for relative and absolute quantitation (iTRAQ) technology, proteomics assessments of human ligamentum flavum (LF), and successive assays were performed to explore the effect of clusterin (CLU) upregulation on LFH pathogenesis. LFH samples exhibited higher cell positive rates of the CLU, TGF-β1, α-SMA, ALK5 and p-SMAD3 proteins than non-LFH samples. Mechanical stress and TGF-β1 initiated CLU expression in LF cells. Notably, CLU inhibited the expression of mechanical stress-stimulated and TGF-β1-stimulated COL1A2 and α-SMA. Mechanistic studies showed that CLU inhibited mechanical stress-stimulated and TGF-β1-induced SMAD3 activities through suppression of the phosphorylation of SMAD3 and by inhibiting its nuclear translocation by competitively binding to ALK5. PRKD3 stabilized CLU protein by inhibiting lysosomal distribution and degradation of CLU. CLU attenuated mechanical stress-induced LFH in vivo. In summary, the findings showed that CLU attenuates mechanical stress-induced LFH by modulating the TGF-β1 pathways in vitro and in vivo. These findings imply that CLU is induced by mechanical stress and TGF-β1 and inhibits LF fibrotic responses via negative feedback regulation of the TGF-β1 pathway. These findings indicate that CLU is a potential treatment target for LFH.

Investigation on the morphology of hierarchical mesoporous ZSM-5 zeolite prepared by the CO2-in-water microemulsion method

Chunlei Wang,Juntao Yan,Jianfen Li,Jianmin Sun 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.9

A series of hierarchical mesoporous ZSM-5 zeolites with different morphology were successfully syn-thesized by the CO2-in-water microemulsion method, and mesoporosity was formed without organotemplate. The dif-ferent synthesis conditions, including silica alumina molar ratio, stirring time and compressed CO2 pressure, were sys-tematically investigated to discuss the influence of these conditions on the morphology of ZSM-5 zeolite. The resultingsamples were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), inductively coupledplasma (ICP) and nitrogen adsorption-desorption measurement. XRD results indicated that compressed CO2 route forthe synthesis of MFI zeolites had a fast crystallization rate and good crystallinity. SEM images showed that the ZSM-5hierarchical mesoporous ZSM-5 zeolite had a uniform chain-like crystal morphology, whereas silicalite-1 displayed amonodisperse crystal morphology. In addition, the nitrogen adsorption-desorption measurement provided sufficientevidence for the presence of hierarchical mesopores in ZSM-5 zeolite.

Rhamnolipids amendment improves soil properties and enhances microecological functions in the saline-alkali soil

Shichen Li,Hanghai Zhou,Chunlei Chen,Feng Zeng,Gang Zheng,Xingpeng Wang,Chunfang Zhang 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.4

A laboratory-scale study was conducted to investigate the effect of rhamnolipids (RLs) supplement on the amelioration of saline-alkali soils. The RLs supplement improved the soil aggregates stability and promoted the formation of macro-aggregates which increased by up to 10.84% and 15.92% in alkaline soil and saline soil, respectively. In addition, RLs amendment led to a pH reduction from initial 8.87 to 7.80-7.84 in alkaline soil, and a salt rejection up to 20.72% in saline soil, remarkably alleviating the saline-alkali stress on microorganisms and plants. Meanwhile, microbial growth and activity as well as the seed germination performance were greatly improved in both types of soil. Furthermore, RLs addition greatly altered the microbial community structure and supported the proliferation of bacterial species (e.g., Pseudomonas oleovorans, Pseudomonas stutzeri, and Alcanivorax dieselolei) that favored the improvement of soil properties and nutrients circulation, thus markedly enhancing the microecological functions including carbon and nitrogen metabolisms. Further supplement of γ-PGA only exhibited promoting effect on aggregates formation and microbial growth and activity. The findings obtained in this study prove the application of RLs as a promising approach for saline-alkali soil amelioration.

Sampling of Bemisia tabaci adults using a pre-programmed autonomous pest control robot

정부근,XIA CHUNLEI,송유한,이장명,Yan Li,김흥수,전태수 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.4

An autonomous robot (Cabbot)was built to control insect pests in a plastic greenhouse and utilized to sample adultwhiteflies (Bemisia tabaci) on paprika plants (Capsicum annum var. angulosum). To accomplish this, a sampling deviceconsisting of an air compressor for pest agitation, a sticky trap (100mm×150mm) and an image processingsystem for pest identification were installed on the Cabbot. The sampling precision of the Cabbot (D= 0.16)washigher than that of the sticky trap (D= 0.19) when sampling of adultwhite flies was conducted in caged pots. TheCabbot could also collect a substantial number of individuals within a short duration (3 minutes). The collection efficiency(i.e., percent of samples collected at least n (1 in this case) individuals to the total sample number) of insectsby the Cabbot was markedly high at low population size, showing approximately 30% when the populationsize was ≤16 individuals per plant. The sampling precision and collection efficiency suggested that the Cabbot iseffective in local, short-term sampling and could be used for early warning of pest occurrences.

Experimental Study on Dynamic Interaction between Large U-Shape Aqueduct and Water

Haibo Wang,Chunlei Li,Lijun Zhao 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.3

Hydrodynamic interaction between water and structure plays an important role in the evaluation of seismic response of aqueducts in area of high seismicity. This interaction differs in axial, lateral and vertical direction of aqueduct. Both impulsive and convective effects of water exist in lateral direction. In presented study, shaking table tests were conducted with a1/10 model of U-shape aqueduct, of 30 m long, to study the hydrodynamic interaction. With the analyses of aqueduct and water responses to stationary White Noise excitations as well as non-stationary excitations, impulsive and convective effects of water were deduced quantitatively. Characteristic frequencies and corresponding modes of aqueduct were identified with the transfer functions of acceleration, sloshing frequencies in lateral direction were recognized with the transfer functions of seat force. Impulsive mass was deduced from the alteration in fundamental frequency and the corresponding modal seat forces of the aqueduct with and without water. Convective mass was obtained from the wave height of sloshing and lateral seat forces recorded, or the lateral modal seat force and damping ratio. Comparison was made with those given in simplified methods for rigid structure. Observed hydrodynamic pressure on the structure in the test was smaller than that based on the rigid assumption.

Rhizosphere Microbial Community and Metabolites of Susceptible and Resistant Tobacco Cultivars to Bacterial Wilt

Zhao Wan,Li Yanyan,Yang Chunlei,Yang Yong,Hu Yun 한국미생물학회 2023 The journal of microbiology Vol.61 No.4

Soil-borne diseases are closely related to rhizosphere microecosystem. While, plant species and genotypes are important factors affected rhizosphere microecosystem. In this study, the rhizosphere soil microbial community and metabolites of susceptible and resistant tobacco cultivars were investigated. The results showed that there were significant differences in the rhizosphere microbial community and metabolites between susceptible cultivar Yunyan87 and resistant cultivar Fandi3. Furthermore, the rhizosphere soil of Fandi3 showed a higher microbial diversity than that of Yunyan87. The abundance of R. solanacearum was much higher in the rhizosphere soil of Yunyan87 than in the rhizosphere soil of Fandi3, resulting in a higher disease incidence and index. While the abundance of beneficial bacteria in the rhizosphere soil of Fandi3 were higher than that of Yunyan87. Additionally, there were significant differences in metabolites between Yunyan87 and Fandi3 cultivars, and 4-hydroxybenzaldehyde, 3-hydroxy-4-methoxybenzoic acid, vamillic aldehyde, benzoic acid, 4-hydroxybenzyl alcohol, p-hydroxybenzoic acid and phthalic acid were notably high in Yunyan87. Redundancy analysis (RDA) indicated that the rhizosphere microbial community of Fandi3 and Yunyan87 were highly correlated with various environmental factors and metabolites. Overall, susceptible and resistant tobacco cultivars had different impact on rhizosphere microbial community and metabolites. The results expand our understanding of the roles of tobacco cultivars in plant-micro-ecosystem interactions, and provide a basis for the control of tobacco bacterial wilt.

Matrine promotes trophoblast invasion and reduces inflammation via miR-19a-3p to prevent preeclampsia

Yang Weina,Kang Qian,Li Chunlei,Bo Shuchun,Wang Yue 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.3

Background During preeclampsia (PE), reactive oxygen species (ROS) production and infl ammation are increased. Matrine exerts diverse pharmacological eff ects, including anti-infl ammatory and anti-apoptotic eff ects. Objective The present study aimed to study the therapeutic eff ects of matrine on PE. Human trophoblasts (HTR-8/SVneo cells) were treated with H 2 O 2 to simulate oxidative stress, followed by treatment with various concentrations of matrine. Next, fl ow cytometry was used to examine apoptosis. Cell invasion and migration were examined by Transwell and scratch wound assays, respectively. The concentrations of IL-6, IL-8, and TNF-α were quantifi ed by enzyme-linked immunosorbent assays. The expression levels of miR-19a-3p and ZEB2 were quantifi ed by qRT-PCR. The interaction between miR-19a-3p and ZEB2 was examined by a dual-luciferase reporter assay. Result Matrine attenuated H 2 O 2 -induced increases in ROS levels, apoptosis, and cytokine release in HTR/SVneo cells. Matrine also increased cell migration and invasion during oxidative stress by reducing miR-19a-3p expression. Moreover, miR-19a-3p can target ZEB2 directly. Conclusion During oxidative stress, matrine can reduce ROS levels, apoptosis, and infl ammation in human trophoblasts and promote migration and invasion via miR-19a-3p, off ering a potential new approach for the treatment of PE.