기도이물에 관한 임상적 고찰

한찬수,김태정,김일호,김유재,김천숙,안기량 순천향의학연구소 1996 Journal of Soonchunhyang Medical Science Vol.2 No.2

Aspiration of a foreign body in the airway is common cause of sudden obstructive breathing, crouping cough, hoarseness, and wheezing. Especially that caused serious problem in case of the young children. A past history of foreign body aspiration is itself an indication for bronchoscopic examination of the airway, because some children with aspirated foreign bodies are without symptoms and chest X-ray films may not show abnormalities. So the anesthesiologist should take a notice of removing foreign body in the anesthetic procedure. The author have experienced 36 cases removal of foreign bodies in the airway under general anesthesia from July, 1982 to January, 1996 in Chunan Hospital, College of Medicine, Soonchunhyang University. The results were as follows 1) Among the 36 cases, 25 cases(69.7%) were under the age of 5. The ratio of male and female was 1.8 : 1. 2) In duration of lodgement, there was most frequent within one day in 15 cases(41.7%) and the longest duration was 20 days. 3) The most common symptom, sign and auscultation finding were cough 19 cases(52.8%). chest retractions 7 cases(19.4%) and decreased breathing sound 20 cases(55.6%) 4) Abnormalities in the chest X-ray were found in 15 cases(41.7%) among 36 cases. 5) Vegetable foreign bodies, expecially peanut were the most common airway foreign body(28.7%), followed by plastic foreign body(14.4%) and mucous plug(11.3%). 6) The foreign body involved the right bronchus 16 cases(45.7), left bronchus 10 cases(28.6%), trachea 5 cases(14.3%), and larynx 4 cases(11.4%). 7) Foreign body induced complications occurred in 9 cases(25.0%); pneumonia 8 cases and pneumomediastinum 1 case, and all of postoperative complication was pneumonia(25.0%). 8) 34 foreign objects(94.4%) were removed by endoscopy but tracheostomy was required in two patients.

The tyrosine kinase inhibitor nintedanib activates SHP-1 and induces apoptosis in triple-negative breast cancer cells

Chun-Yu Liu,Tzu-Ting Huang,Pei-Yi Chu,Chun-Teng Huang,Chia-Han Lee,Wan-Lun Wang,Ka-Yi Lau,Wen-Chun Tsai,Tzu-I Chao,Jung-Chen Su,Ming-Huang Chen,Chung-Wai Shiau,Ling-Ming Tseng,Kuen-Feng Chen 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Triple-negative breast cancer (TNBC) remains difficult to treat and urgently needs new therapeutic options. Nintedanib, a multikinase inhibitor, has exhibited efficacy in early clinical trials for HER2-negative breast cancer. In this study, we examined a new molecular mechanism of nintedanib in TNBC. The results demonstrated that nintedanib enhanced TNBC cell apoptosis, which was accompanied by a reduction of p-STAT3 and its downstream proteins. STAT3 overexpression suppressed nintedanib-mediated apoptosis and further increased the activity of purified SHP-1 protein. Moreover, treatment with either a specific inhibitor of SHP-1 or SHP-1-targeted siRNA reduced the apoptotic effects of nintedanib, which validates the role of SHP-1 in nintedanib-mediated apoptosis. Furthermore, nintedanib-induced apoptosis was attenuated in TNBC cells expressing SHP-1 mutants with constantly open conformations, suggesting that the autoinhibitory mechanism of SHP-1 attenuated the effects of nintedanib. Importantly, nintedanib significantly inhibited tumor growth via the SHP-1/p-STAT3 pathway. Clinically, SHP-1 levels were downregulated, whereas p-STAT3 was upregulated in tumor tissues, and SHP-1 transcripts were associated with improved disease-free survival in TNBC patients. Our findings revealed that nintedanib induces TNBC apoptosis by acting as a SHP-1 agonist, suggesting that targeting STAT3 by enhancing SHP-1 expression could be a viable therapeutic strategy against TNBC.

Nucleophosmin modulates the alleviation of atopic dermatitis caused by the marine-derived compound dihydroaustrasulfone alcohol

Han-Chun Hung,Chien-Wei Feng,Yen-You Lin,Chun-Hong Chen,Kuan-Hao Tsui,Wu-Fu Chen,Chieh-Yu Pa,Jyh-Horng Sheu,Chun-Sung Sung,Zhi-Hong Wen 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Atopic dermatitis (AD) is a chronic inflammatory skin disease, and its prevalence is increasing. AD usually elicits skin barrier dysfunction, dry skin and itching. As the mechanisms of AD remain unknown, there is an urgent need to find effective therapies. Because of the diversity and complexity of marine environments, the discovery of drugs from marine organisms as novel therapeutic agents for human diseases has seen renewed interest. Dihydroaustrasulfone alcohol (WA-25), the synthetic precursor of austrasulfone, which is a natural product isolated from a Formosan soft coral, has been shown to possess many therapeutic effects in our previous studies. However, the detailed mechanisms and therapeutic effects of WA-25 on AD are incompletely understood. We performed in vitro and in vivo studies to examine the effects of WA-25 on AD. We showed that WA-25 blocks inflammation and oxidative stress. Simultaneously, we also found that WA-25 reduces the AD scores and AD-induced transepidermal water loss (TEWL), scratching behavior, and alloknesis. WA-25 is more effective in cases of AD than are the drugs that are currently used clinically. Importantly, we also found that when nucleophosmin (NPM) was inhibited or when its expression was reduced, the anti-inflammatory and anti-AD effects of WA-25 were blocked. These data suggest that NPM plays dual roles in inflammation and AD. Overall, these results suggest that WA-25 is a potential anti-inflammatory and AD therapeutic agent that is modulated by NPM.

Screening for Metastatic Osteosarcoma Biomarkers with a DNA Microarray

Diao, Chun-Yu,Guo, Hong-Bing,Ouyang, Yu-Rong,Zhang, Han-Cong,Liu, Li-Hong,Bu, Jie,Wang, Zhi-Hua,Xiao, Tao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Objective: The aim of this study was to screen for possible biomarkers of metastatic osteosarcoma (OS) using a DNA microarray. Methods: We downloaded the gene expression profile GSE49003 from Gene Expression Omnibus database, which included 6 gene chips from metastatic and 6 from non-metastatic OS patients. The R package was used to screen and identify differentially expressed genes (DEGs) between metastatic and non-metastatic OS patients. Then we compared the expression of DEGs in the two groups and sub-grouped into up-regulated and down-regulated, followed by functional enrichment analysis using the DAVID system. Subsequently, we constructed an miRNA-DEG regulatory network with the help of WebGestalt software. Results: A total of 323 DEGs, including 134 up-regulated and 189 down-regulated, were screened out. The up-regulated DEGs were enriched in 14 subcategories and most significantly in cytoskeleton organization, while the down-regulated DEGs were prevalent in 13 subcategories, especially wound healing. In addition, we identified two important miRNAs (miR-202 and miR-9) pivotal for OS metastasis, and their relevant genes, CALD1 and STX1A. Conclusions: MiR-202 and miR-9 are potential key factors affecting the metastasis of OS and CALD1 and STX1A may be possible targets beneficial for the treatment of metastatic OS. However, further experimental studies are needed to confirm our results.

《说文解字》刀部刑罚相关汉字考察

왕위한(Wang, Yu-Han)(王钰,涵),이춘영(Lee, Chun-Young)(李春永) 대한중국학회 2021 중국학 Vol.74 No.-

刑罚是统治阶级惩戒罪犯、警醒世人的重要手段。据中国历史文献记载,夏朝已经具备一套成体系的刑罚制度。肉刑的行刑过程离不开各类刀具的使用,因此中国古代的刑罚与“刀”有着密不可分的关系。《说文解字》包含大量和刑罚有关的汉字,这些汉字对于明确古代刑罚种类和内容、探究古代文化生活有着重要的意义和价值。《说文解字》刀部字涉及刑罚的汉字共有8个字。其中“㓷”、“劓”代表“劓刑”,“刖”代表“剕刑”,“ ”、“刭”和“刎”则是“大辟”的一种。“刵”作为割耳刑罚,与意义相近的“聝”、“取”等字联系紧密。“㓷”和“劓”作为割鼻刑罚仅在汉代以前使用广泛。“刖”表示割腿刑罚,古代又有“剕”、“跀”等字表示相同意义,同时又与意义相近的“膑”有所差别。“ ”、“刭”和“刎”三字均表示砍头的刑罚。“罚”则表示对情节较轻犯罪行为的处罚,与表示对严重犯罪行为予以惩戒的“刑”字共同构成汉语中的“刑罚”一词。 Punishment is an important method for the ruling class to discipline criminals and alert people. According to historical records, a systematic punishment system was already existent during the Xia Dynasty. The “Ancient Five Punishments” recorded in Book of Documents occupies an important position in ancient Chinese punishments. Because they were all corporal punishment and the execution methods were extremely cruel so they were abolished in the Han Dynasty. The execution process of corporal punishment depends on the use of various knives. Therefore, punishment in ancient China is closely related to the “knife”. Shuo Wen Jie Zi contains a large number of Chinese characters related to punishment, and they are closely related to the “Ancient Five Punishments”. “刵(Er)”, as an ear-cutting penalty, can be traced back to the Zhou Dynasty or even earlier, and has similar meaning with the words “聝(Guo)” and “取(Qu)”. “㓷(Yi)” and “劓(Yi)” are variants of each other, and they were widely used as a penalty for cutting the nose only before the Han Dynasty. “刖(Yue)” means the punishment of cutting the legs, which has same meaning with the words “剕(Fei)” and “跀(Yue)”, but it was different from the word of “膑(Bin)” though they have similar meaning. The three characters “ (Xing)”, “刭(Jing)” and “刎(Wen)” can be explained by each other, and they all refer to the punishment of beheading. “罚(Fa)” means punishment for minor crimes. This word and the word of “刑(Xing)” meaning the punishment for serious crimes constitute the phrase of “刑罚(Xingfa)” in Chinese.

Ankrd7, a Novel Gene Specifically Expressed in Sertoli Cells and Its Potential Roles in Sertoli Cell Maturation

Yu-Qiang Shi,Lian-Cai Du,Qing-Zhong Wang,Chun-Fang Han 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.2

The somatic Sertoli cells play an essential role in testis determination and spermatogenesis by providing nutrition and structural support. In the current study, we report on the novel Ankrd7 gene that contains five ankyrin repeat domains. This gene was specifically expressed in Sertoli cells and was regulated in a maturation-dependent manner. Its expression was restricted to testicular tissue, and its mRNA could be detected in testes at as early as 14 dpp (days post partum) using RT-PCR analysis. In both testicular tissue sections and in vitro cultured Sertoli cells, the Ankrd7 protein was localized to the nucleus of the Sertoli cell. Immunohistochemistry and immunocytochemistry investigations showed that the protein was detectable in testicular tissues at 20 dpp, at which time Sertoli cells were gradually differentiating into their mature cellular form. These results suggest that Ankrd7 is probably involved in the process of Sertoli cell maturation and in spermatogenesis.

Progress in Studies and Development of Immunoregulation Functional Food and Their Functional Component in China

Chun-ji Han,Zhen-yu Quan,Guo-zhe Jiang 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Synthesis and Viscoelastic Behavior of Non-Stoichiometric Spinel Ferrite Particle Suspension

Han, Jae Kyeong,Han, Wen Jiao,Gao, Chun Yan,Dong, Yu Zhen,Choi, Hyoung Jin IEEE 2018 IEEE transactions on magnetics Vol.54 No.11

<P>Magnetorheological (MR) fluid with tunable and reversible phase transition is one of the most promising smart materials for engineering applications. In this paper, to improve the MR performance of ferrites, non-stoichiometric Zn-doped spinel ferrite (Zn<SUB>0.417</SUB>Fe<SUB>2.583</SUB>O<SUB>4</SUB>) nanoparticles with a high saturation magnetization and a truncated octahedron-like shape were synthesized using a thermal decomposition process. The crystallographic structure was analyzed using X-ray diffraction and field emission transmission electron microscope image. The Zn<SUB>0.417</SUB>Fe<SUB>2.583</SUB>O<SUB>4</SUB> had a high saturation magnetization value of 101 <TEX>$\text {A}\cdot \text {m}^{2}$</TEX>/kg at 933 kA/m, showing higher saturation magnetization than other ferrites. The MR characteristics of the Zn<SUB>0.417</SUB>Fe<SUB>2.583</SUB>O<SUB>4</SUB> particles suspended in silicone oil were studied using a rotation rheometer under various magnetic field strengths. This MR fluid exhibited typical MR characteristics, and its flow curves were fit well with the Herschel–Bulkley model.</P>

Isolation and Characterization of a Type Ⅱ Peroxiredoxin Gene from Panax ginseng C. A. Meyer

Yu-Jin Kim,Jung-Hye Lee,Ok Ran Lee,Ju-Sun Shim,Seok-Kyu Jung,Na-Ri Son,Ju-Han Kim,Se-Young Kim,Deok-Chun Yang 고려인삼학회 2010 Journal of Ginseng Research Vol.34 No.4

A peroxiredoxin cDNA (PgPrx) was isolated and characterized from the leaves of Panax ginseng. The cDNA is 716 nucleotides long and has an open reading frame of 489 base pairs with a deduced amino acid sequence of 162 residues. The calculated molecular mass of the mature protein is approximately 17.4 kDa with a predicted isoelectric point of 5.37. A GenBank BlastX search revealed that the deduced amino acid sequence of PgPrx shares a high degree homology with type Ⅱ peroxiredoxin (Prx) proteins in other plants. The PgPrx gene was highly expressed in leaves, and expressed at a low level in the stem. To analyze the gene expression of PgPrx in response to various abiotic stresses, we utilized real-time quantitative RTPCR. Our results reveal that PgPrx expression is induced by ultraviolet irradiation, low temperature, and salt. The induction of PgPrx in response to abiotic stimuli suggests that ginseng Prx may function to protect the host against environmental stresses.

Association of GnRH1 Polymorphisms with Rheumatoid Arthritis in a Korean Female

Yu Mi Kim(김유미),Kye Young Han(한계영),Eun Bi Kwak(곽은비),Wanjoo Chun(전완주),Sung Soo Kim(김성수),Hee Jae Lee(이희제) 대한정형외과학회 2010 대한정형외과학회지 Vol.45 No.5

목적: 류마티스 관절염(RA)의 병태생리에 대해 아직 명확하게 밝혀져 있지 않으나 일부의 경우 유전적 요인이 류마티스 관절염의 발생에 영향을 주는 것으로 알려져 있다. 역학조시 결과 여성이 남성보다 류마티스성 관절염의 유병률이 3배 정도 높다. Gonadal 호르몬들은 면역체계나 자가면역질환에서 성에 따른 이형을 나타내는데 영향을 주는 것으로 알려져 있다. 이 가운데 gonadotrophin-releasing hormone (GnRH)는 면역 시스템을 조절하는 데도 중요한 역할을 한다. 따라서,GnRH가 류마티스 관절염에서 성적 차이를 나타내는데 중요한 역할을 할 수 있으며 이를 증명하기 위해 GnRH1 유전자를 후보 유전자로 선택하고 이들 유전자 내의 단일염기다형성(SNP)을 분석함으로써,RA와 GnRHl 유전자 다형성과의 연관성에 대해 연구하고자 하였다. 대상 및 방법: 153명의 RA 여성 환자와 96명의 정상 대조군을 대상으로, GnHR1 유전자 내의 5개의 SNP, rs2659590, rs2321248, rs6186, rs6185, rs2321049의 유전자형과 대립유전자형의 빈도를 비교 분석하였다. 또한 RA 환자들을 erythrocyte sedimentation rate (ESR)과 골 미란 유무에 따라 나누고 GnRH1 SNP과의 연관성을 확인하였다. 결과: 한국인 여성 RA 환자들과 정상군들 사이에서 GnRH1 SNP들의 유전자형과 대립유전자들의 빈도는 유의한 차이가 없었다. 하지만, rs2659590, rs6185, rs2321248에서 ESR이 30 이상인 환자와 30 미만 환자 사이에 로지스퇴 회귀 분석 결과 유의한 상관관계가 나타났다. 하지만, 골 미란 유무는 연관성이 없었다. 결론: 본 연구에서 RA와 GnRH1 유전자 다형성 사이의 연관성은 보여주지 못하였지만, ESR의 수치와 GnRH1 유전자 다형성 간에 연관성을 보였다. 비록 그 수가 적어 제한적인 의미를 가질 수 있으나 처음으로 GnRH1 유전자의 다형성과 RA환자의 ESR 간의 연관 가능성을 시사하는데 의의가 있다. Purpose: Rheumatoid arthritis (RA) is a common, chronic inflammatory arthritis that develops most often in women. Gonadal hormones may account for the sexual dimorphism in the immune response and for the greater incidence of autoimmune disease in females. Gonadotrophin- releasing hormone (GnRH), one of the gonadal hormones, plays an important role in immune system modulation. This study examined the effects of single nucleotide polymorphisms (SNP) in GnRH on gender differences in the pathophysiology of RA. Materials and Methods: The presence of SNPs rs2659590, rs2321248, rs6186, rs6185, and rs2321 049 in the human GnRH1 gene was confirmed in Korean RA patients by Taqman" SNP genotyping assays A total of 153 unrelated female, Korean RA patients and 96 female Korean controls participated. Results: There were no significant associations between GnRH1 polymorph isms and RA. However, we found that the rs2659590, rs6185 and rs2321248 polymorphism might be associated with a susceptibility to aberrantly high erythrocyte sedimentation rates in female RA patients. Conclusion: Additional studies, with a larger number of patients and in different populations will be required to assess whether GnRH1 polymorph isms and these haplotypes could be used as susceptibility or resistance markers in RA. To our knowledge, this study is the first to analyze associations between SNPs of GnRH1 and RA.