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Lee, Hangyeore,Mun, Dong-Gi,So, Jeong Eun,Bae, Jingi,Kim, Hokeun,Masselon, Christophe,Lee, Sang-Won American Chemical Society 2016 ANALYTICAL CHEMISTRY - Vol.88 No.23
<P>Proteomics aims to achieve complete profiling of the protein content and protein modifications in cells, tissues, and biofluids and to quantitatively determine changes in their abundances. This information serves to elucidate cellular processes and signaling pathways and to identify candidate protein biomarkers and/or therapeutic targets. Analyses must therefore be both comprehensive and efficient. Here, we present a novel online two-dimensional reverse-phase/reverse-phase liquid chromatography separation platform, which is based on a newly developed online noncontiguous fractionating and concatenating device (NCFC fractionator). In bottom-up proteomics analyses of a complex proteome, this system provided significantly improved exploitation of the separation space of the two RPs, considerably increasing the numbers of peptides identified compared to a contiguous 2D-RP/RPLC method. The fully automated online 2D-NCFC-RP/RPLC system bypassed a number of labor-intensive manual processes required with the previously described offline 2D-NCFC RP/RPLC method, and thus, it offers minimal sample loss in a context of highly reproducible 2D-RP/RPLC experiments.</P>
Bio-Frontier Symposia : Proteomics ; Advanced strategies for protein profiling using LC-FTICR/MS
( Ljiljana Pasa Tolic ),( Gordon A. Anderson ),( Mary S. Lipton ),( David G. Camp ),( Yu Feng Shen ),( Christophe Masselon ),( Richard D. Smith ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-
Bio-Frontier Symposia : Proteomics ; Advanced strategies for protein profiling using LC-FTICR/MS
( Ljiljana Pasa Toli ),( Gordon A. Anderson ),( Mary S. Lipton ),( David G. Camp II ),( Yu Feng Shen ),( Christophe Masselon ),( Richard D. Smith ) 한국생화학분자생물학회 (구 한국생화학회) 2005 62회 KSBMB Annual Meeting in 2005 Vol.- No.-