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( Abimbola Comfort Badejo ),( Won Hyong Chung ),( Nam Shin Kim ),( Se Kye Kim ),( Jin Choul Chai ),( Young Seek Lee ),( Kyoung Hwa Jung ),( Hyo Joon Kim ),( Young Gyu Chai ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.9
Mycobacterium gilvum PYR-GCK is a bacterial strain under study for its bioremediation use on heavy hydrocarbon pollutants in the environment. During the course of our study, mammalian cell entry (mce) genes, known to facilitate pathogenicity in M. tuberculosis, were highly expressed during a comparative and substrate-related cultural global transcriptomic study. RNA sequencing of the global transcriptome of the test strain in two different substrates, pyrene and glucose, showed high expression of the mce genes based on the differential results. After validating the expression of these genes with quantitative real-time PCR, we arrived at the conclusion that the genes were expressed based on the pyrene substrate (a phytosterol compound), and sterol metabolism is said to activate the expression of the mce genes in some actinomycetes bacteria, M. gilvum PYR-GCK in this case. This study is believed to be important based on the fact that some mycobacterial strains are undergoing a continuous research as a result of their use in practical bioremediation of anthropogenic exposure of toxic organic wastes in the environment.
Transcriptomic study of mouse embryonic neural stem cell differentiation under ethanol treatment
Mandal, Chanchal,Park, Ji Hyun,Choi, Mi Ran,Kim, Sun Hwa,Badejo, Abimbola Comfort,Chai, Jin Choul,Lee, Young Seek,Jung, Kyoung Hwa,Chai, Young Gyu Springer-Verlag 2015 MOLECULAR BIOLOGY REPORTS Vol.42 No.7