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- 저자 : Chia Yun Tseng (검색결과 1 건)
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AB208: A Chimeric Ligand With Superior Differentiation Capacity to Produce Insulin
Chia Yun Tseng Gachon university 2013 국내석사
Adult stem cells have great potential application for the therapeutic approach in the field of regenerative medicine. Due to their easy access and ethical issue, adipose‐derived stem cell has become increasingly popular. Many studies have stated Activin A, one of the TGF‐ β family, played a critical role for stem cell differentation to insulin producing cell. AB208, achimeric ligand of Activin‐bA and BMP‐2, exhibits the refolding characteristics of BMP‐2 while possessing Activin‐like signaling attributes. We isolated adipose‐derived stem cell from canine fat tissues and used AB208 to replace Activin A in two step induction protocol based on DMEM/F12, N2 supplement , B27 supplement, FGF2, nicotinamide, exdenin‐4, HGF and valproic acid for a duration of 16 days. After differentiation, the expression of genes related to β‐cell differentiation, INS, PDX‐1, and NGN‐3, was determined by real‐time PCR. The cASCs incubated with AB208 showed 2.5 times higher gene expression than these incubated with Activin A. The result of glucose challenage test of differentiation cells shows that cASCs differentiated with AB208 secreat more insulin than these differentiated with Activin A. These initail data show that AB208 has differentiation capacity to produce insulin and could be used as a therapeutic approach to treat Diabetes in the future. Key word: canine ASC, insulin producing cell/ cluster, Activin A , Type 1 Diabetes, pancreatic islet
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