韓國産 복어 毒의 毒性學的 硏究 = Toxicological study on the purified trtrodotoxin from fugu ocellatus obscurum

다국어 초록 (Multilingual Abstract)

Tetrodon poisoning, also known as fugu poisoning, occurs chiefly in countries surrounding the South China Sea. It has been known for long time that tetrodotoxin which is the purified toxic principle is responsible for the poisoning. Crystalline tetrodotoxin is the most potent of the substances that can interfere with the production of action potentials in excitable cells and also unique in acting only on changes in sodium permeability.
It was reported that the occurrence of tetrodotoxin as well as distribution in the tissues is varied from species to species. Furthermore, the seasonal variation of toxicity was observed in ovaries of female tetrodon fish.
Fugu ocellatus obscurum which is one species of tetrodon families inhabit only around Yellow Sea of Korea and Yantzu-Chiang of China. In this study, tetrodotoxin was extracted and purified from Fugu ocellatus obscurum and the toxicity of the tetrodotoxin was compared with the toxin from other species. In addition the toxicological and pharmacological tests were performed and the results were summerized as followings.
1. Among the various tissues of Fugu ocellatus obscurum, ovaries, liver and bile juice exhibited the potent toxicity while skin and testis of tetrodon fish showed relatively low toxicity.
2. LD_(50) of the crude tetrodotoxin was about 11.8 μg/kg and that of the purified toxin was 11.2 μg/kg
3. Infrared spectrum of the purified tetrodotoxin was almost exactly same pattern as the spectrum of standard tetrodotoxin.
4. The twitch tension of gastrocnemius muscle induced by tibial nerve stimulation was reduced gradually by the cumulative tetrodotoxin(5 μg/kg every 10 minutes) upto 30 minutes, and then the tension was completly abolished. The blood pressure and heart rate, however, showed maximal depression at the final concentration of 10 μg/kg of the tetrodotoxin, and no further depression thereafter.
5. In he isolated rabbit atria, the tetrodotoxin produced minimal depression. In the isolated rabbit duodenum, a large doses of tetrodotoxin caused dose-related increase of motility. But pretreatment with tetrodotoxin did not affect the acetylcholine or norepinephrine response in the isolated duodenum.

목차 (Table of Contents)

• 목차
• I. 서론 = 2
• II. 실험 = 4
• 1. 실험재료 = 4
• 2. 시약 및 기기 = 4
• 3. 황복의 각 조직부위별 독성시험 = 4
• 4. Tetrododtoxin 의 추출 및 정제 = 5
• 1) Tetrododtoxin의 추출 = 5
• 2) Tetrododtoxin의 정제 = 5
• 5. Tetrododtoxin 의 同定 = 5
• 1) Mouse에 대한 LD_(50) 실험 = 5
• 2) Tetrododtoxin 의 IR spectrum = 5
• 3) Tetrododtoxin 및 그 dansyl염의 TLC = 5
• 4) Alkali분해물의 TLC 및 UV spectrum = 6
• 6. Tetrododtoxin의 동물실험 = 6
• 1) 혈압 및 골격근에 대한 생체실험 = 6
• 2) 척출 심방 및 장관에 대한 실험 = 7
• III. 결과 및 고찰 = 8
• IV. 결론 = 15
• 參考文獻 = 16
• SUMMARY = 18