Study on the tissue-specific alternative splicing patterns of Alzheimer's disease mouse model via transcriptome analysis

목차 (Table of Contents)

• 1. INTRODUCTION 1
• 1.1. Alzheimer's disease 1
• 1.2. Mild cognitive impairment (MCI) 6
• 1.3. Mouse model of Alzheimer's disease 9
• 1.4. The diagnosis of AD 12
• 1.5. Alternative splicing 14
• 1.6. Intron retention 17
• 1.7. View of this study 21
• 2. MATERIALS AND METHODS 24
• 2.1. Animals 24
• 2.2. Immunocytochemistry 24
• 2.3. Total RNA isolation and RT-qPCR 25
• 2.4. Library preparation and RNA sequencing 26
• 2.5. Bioinformatical analysis 26
• 2.6. Statistical analysis 27
• 3. RESULTS 30
• 3.1. Establishment of 5XFAD transgenic (5XFADTg/0) mouse 30
• 3.2. GFAP expression in the Hippocampus and the cerebral cortex of WT and 5XFAD Mice 34
• 3.3. A(1-42) expression and deposition in hippocampus and cerebral cortex of WT and 5XFAD mice 39
• 3.4. GFAP expression in the retina of WT and 5XFAD mice 44
• 3.5. Amyloid beta depositions in the retina of WT and 5XFAD mice 48
• 3.6. Assess visual function of WT and 5XFAD though ERG (electroretinography) 51
• 3.7. Dynamic of transcriptome profiling of 5XFAD mice using RNA-seq technique 56
• 3.8. Experimental validation of IR at WT and 5XFAD genes 60
• 4. DISCUSSION 66
• REFERENCES 69
• 국문초록 74