Epigallocatechin-3-O-gallate(EGCG) attenuates the hemodynamics stimulated by caffeine through decrease of catecholamines release in human and rats = Epigallocatechin-3-O-gallate(EGCG)가 카테콜라민 분비 감소를 통해서 카페인으로 자극된 혈류운동성의 완화에 대한 연구

다국어 초록 (Multilingual Abstract)

Human study was planned to know the counteractions between caffeine and Epigallocatechin-3-O-gallate (EGCG) on hemodynamics. Caffeine tablets (200 mg) were orally administered to healthy men aged between 25-35 years 30 min after the oral administration of EGCG (100 and 200 mg).
The increase in BP induced by caffeine was inhibited when co-administrated with EGCG. Caffeine increased blood catecholamines (noradrenaline, adrenaline and dopamine) levels, but EGCG inhibited the increase in noradrenaline, adrenaline and dopamine levels induced by caffeine.
On the other hand, it was investigated whether EGCG decreases the elevated heart rate (HR) and systolic perfusion pressure, and ventricular contractility induced by adrenergic agonists in the isolated rat heart. The modified Krebs-Henseleit solution was perfused through a Langendorff apparatus to the isolated hearts of rats. HR, systolic perfusion pressure, and developed maximal rates of contraction (+dP/dtmax) and relaxation (−dP/dtmax) were increased by epinephrine (EP) and isoproterenol (IP). By contrast, EGCG decreased the elevated HR, systolic perfusion pressure, and left ventricular +/−dp/dtmax induced by EP and/or IP.
It is interesting that EGCG could attenuate the hemodynamics stimulated by caffeine through decrease of catecholamine release.

국문 초록 (Abstract)

녹차의 주성분인 Epigallocatechin-3-O-gallate(EGCG)이 caffeine의해 증가된 혈압과 심박동수 및 혈중 카테콜라민(noradrenaline, adrenaline, dopamine) 농도를 rat와 인체에서 각각 측정한 결과 다음과 같은 결과를 얻었다.
1. 인체에 caffeine 투여로 증가된 혈압은 EGCG 100 mg/kg과 caffeine 200 mg/kg 병용투여로 유의하게 억제되었고 심박동수는 EGCG 투여로 약간 억제되는 경향을 보였다.
2. 인체에 caffeine 투여로 증가된 혈중 noradrenaline, adrenaline 농도는 EGCG 100 mg/kg과 caffeine 200 mg/kg 병용투여로, dopamine 농도는 EGCG 100 mg/kg과 caffeine 200 mg/kg 병용투여 및 EGCG 200 mg/kg과 caffeine 200 mg/kg 병용투여로 유의하게 억제되었다.
3. Rat의 적출심장에 epinephrine 투여로 증가된 쥐의 심박동수와 systolic perfusion pressure는 EGCG(5 및 10 μM)에 의해, +dP/dtmax pressure는 EGCG(10 μM)에 의해 유의하게 감소되었다.
4. Rat의 적출심장에 isoproterenol 투여로 증가된 쥐의 심박동수와 systolic perfusion pressure는 EGCG(30 μM)에 의해, −dP/dtmax pressure는 EGCG(5 및 10 μM)에 의해 유의하게 감소되었다.
이는 녹차의 주성분인 EGCG가 caffeine에 의해 증가된 혈압과 심박동수를 catecholamine 방출을 억제하는 결과로 사려된다.

목차 (Table of Contents)

• Introduction 1
• Materials and Methods 4
• Drugs 4
• Human study 4
• Animal study 5
• Procedure 6
• Statistics 7
• Results 8
• 1. Human study 8
• 2. Animal experiments 11
• Discussion 15