맨드라미(Celosia cristata) 추출물의 생리활성 및 꽃 색소의 모발 염색성 평가 = Screening on the Physiological Activities of Celosia cristata Extracts and Natural Dyeing Characteristics of Hair Using Flower Pigment

다국어 초록 (Multilingual Abstract)

This study was to examine total contents of Polyphenol and Flavonoid, composition and content of Phenolic Acid and Flavonoid, and antioxidant activity by DPPH with hot water and methanol extracts from flower, flower stalk and stem of Celosia cristata and artificial hair dyeing with flower pigment. The results were as follows:

1. Total Polyphenol content for hot water extract was 385.67 ± 0.76 mg% of flower, 248.33 ± 0.35 mg% of flower stalk, and 93.67 ± 0.24 mg% of stem. And methanol extract was 405.33 ± 0.53 mg% of flower, 224.35 ± 0.77 mg% of flower stalk, and 85.00 ± 0.82 mg% of stem.

2. Total Flavonoid content for hot water extract was 194.50 ± 0.36 mg% of flower which had the highest, 109.30 ± 0.52 mg% of flower stalk, and 19.55 ± 0.23 mg% of stem. And methanol extract was 195.70 ± 0.77 mg% of flower, 115.50 ± 0.87 mg% of flower stalk, and 20.90 ± 0.15 mg% of stem; extract of hot water and methanol are almost same.

3. From hot water and methanol extract for flower, flower stalk and stem of Celosia cristata, we confirm there are 10 types of Phenolic Acids: Protocatechuic Acid, Chlorogenic Acid, 4-Hydroxybenzonic Acid, Caffeic Acid, Isovanillic Acid, p-Coumaric Acid, Ferulic Acid, t-Coumaric Acid, Rosmarinic Acid, and t-Cinnamic Acid, and 4 types of Flavonoid: Rutin, Taxifolin, Quecertin, and Kaempferol, respectively.

4. DPPH radical scavenging activity for hot water extract was 64.85 ± 0.37% of flower which had the highest, 54.69 ± 0.42% of flower stalk, and 8.41 ± 0.22% of stem. And methanol extract was 71.51 ± 0.35% of flower, 36.84 ± 0.51% of flower stalk, and 9.50 ± 0.28% of stem.

5. Dyeing of artificial hair with extracted flower pigment from Celosia cristata was failed when hair did not mordant. In pre-mordanted case, hair color with simultaneous mordant dyeing was light purple visually, and at 40℃, effect of dyeing hair in 30minutes(3% mordanted) was high and dyeing in 60minutes with 2% and 3% of mordant was the highest. And simultaneous mordant in 30minutes and 60minutes at 50℃ was found to very good at 3% of mordant.

6. In fastness of shampoo, dyeing hair in 30minutes and 60minutes at 50℃ are more stable than dyeing in 30minutes and 60minutes at 40℃.
7. In fastness of daylight, dyeing hair in 30minutes and 60minutes at 50℃ are more stable than dyeing in 30minutes and 60minutes at 40℃.

From these results, antioxidant activity of hot water and methanol extracts from flower of Celosia cristata was satisfactory; it maybe useful in the materials of natural antioxidant. But additional connected research between enzyme activity within a cell and interrelation with cosmetics is demanded to use it as cosmetic material.
Also, since flower pigment of Celosia cristata is water soluble, effect of dyeing hair was low. But we anticipate good result if research about mordant or dyeing method continues.

목차 (Table of Contents)

• Ⅰ. 서 론 1
• Ⅱ. 이론적 배경
• 1. 식물 추출물의 생리활성 3
• 2. 페놀계 화합물의 종류 및 특징 4
• 3. 천연염색 5
• 4. 염모제의 종류 및 특성 7
• 5. 매염제의 종류 및 특성 10
• 6. 매염방법의 종류 및 특성 13
• 7. 맨드라미의 특성 13
• Ⅲ. 재료 및 방법
• 1. 실험재료 16
• 2. 시료의 추출 17
• 3. 생리활성 평가 17
• 4. 색소추출 19
• 5. 시료 모발의 탈색시술 20
• 6. 맨드라미 색소 염액 조제 및 모발 염색조건 20
• 7. 염색모발의 견뢰도 측정 22
• 8. 통계분석 24
• Ⅳ. 결과 및 고찰
• 1. 맨드라미 부위별 추출물의 수율 25
• 2. 맨드라미 추출물의 생리활성 26
• 1) 총 Polyphenol 함량 및 Flavonoid 함량 26
• 2) Phenolic Acid 와 Flavonoid의 조성 및 함량 28
• 3) DPPH 라디칼 소거활성 34
• 3. 맨드라미 꽃 색소의 모발염색 특성 36
• 1) 맨드라미 꽃 색소의 모발에 대한 염색 정도 36
• 2) 맨드라미 꽃 색소의 모발에 대한 염색 색도 40
• 3) 염색 후 모발 세정에 따른 견뢰도 45
• 4) 염색 후 일광처리에 따른 견뢰도 47
• Ⅴ. 결 론 49
• 참고문헌 51
• 영문초록 58