리포솜 제제의 Aminolevulinic Acid를 이용한 여드름 치료제 개발 = Absorption of Liposome δ-5-Aminolevulinic Acid to The Cultured Sebocytes and Hair Organs and Its Clinical Effects on Acne

국문 초록 (Abstract)

기존의 ALA와 Lipo-ALA를 비교한 본 연구에서 Lipo-ALA가 배양 피지 세포 및 모낭 기관 배양 피지선으로의 흡수가 잘 되어 PpIX의 발현이 높았고 또한 상대적으로 안정성이 높으며 독성이 낮은 것으로 나타났다. 1% Lipo-ALA를 이용하여 여드름 환자를 치료한 결과 기존의 20% ALA와 비교했을 때 치료 효과에서 큰 차이가 없음을 알 수 있었다. 그러므로 저자들은 리포솜 형태의 ALA를 여드름의 치료에 유용하게 사용할 수 있는 방법 중의 하나라고 생각한다. 또한 Lipo-ALA를 이용한 국소 광역동요법은 비침습적이면서도 미용적인 치료가 가능하며, 반복치료가 가능하다는 점에서 치료의 적용 대상이 점차 확대되고 있으므로 여드름 외에 다른 질환의 치료에 대한 연구 및 광원에 대한 다각적인 연구가 필요하리라 생각한다.

다국어 초록 (Multilingual Abstract)

Photodynamic therapy(PDT) with topical 5-aminolevulinic and(ALA) has been introduced for effective treatment of facial acne. Topical routes for ALA have been explored in photosensitizer delivery system.
We performed this study to evaluate absorption of liposome ALA(Lipo-ALA) into the sebocytes of the cultured sebaceous glands and hair organs. In addition, we evaluated the effectiveness of Lipo-ALA-PDT for facial acne.
We cultured sebocytes which were derived from human sebaceous gland and pilosebaceous hair units which were isolated from occipital scalp. Lipo-ALA and ALA were added to the culture media containing sebocytes and pilosebaceous hair units. And eight patients with mild to moderate acne were treated with one session of topical Lipo-ALA PDT using sun lightand fluorescent lamp, and nineteen patients with mild to moderate acne were treated with three sessions of topical Lipo-ALA PDT with the same light sources. Korean Acne Grading System was used to be evaluated for effectiveness onfacial acne.
Absorption of Lipo-ALA into the cultured sebocytes and hair organs was higher than that of ALA. Of eight patients treated with one session of Lipo-ALA-PDT, three improved, but five were stationary. Of nineteen patients treated with three sessions of Lipo-ALA PDT, twelve improved, but seven were stationary. Lipo-ALA PDT of three sessions was effective in treatment of acne(p<0.05).
Lipo-ALA could be considered to be an effective photosensitizer in photodynamic therapy for facial acne.

목차 (Table of Contents)

• 서론 = 1
• 재료 및 방법 = 3
• 결과 = 8
• 고찰 = 17
• 요약 = 20
• 참고문헌 = 21
• Abstract = 25