Profiling of the dysregulated genes in the extrinsic type of atopic dermatitis : human 34K oligochip microarray study = 外因性 아토피 皮膚炎 患者에서 調節異常 遺傳子의 發現樣相에 關한 分析 : 人間의 34K oligochip microarray 硏究

다국어 초록 (Multilingual Abstract)

Atopic dermatitis (AD) is a chronic inflammatory skin disease associated with eczema distributions, pruritus and dry skin. To gain more insights into the AD pathogenesis, the gene alterations of extrinsic type of AD (ADe) from the patients’ biopsy samples via human 34K-oligochip microarray has been investigated in this study. Significance analysis of microarray (SAM) results showed that 97 genes were up-regulated and 131 genes were down-regulated compared to the normal control subjects with a higher than 2-fold expression change. Several important genes such as cytokines, chemokines, antimicrobial peptides, immune response genes, lipids-related genes, extracellular matrix (ECM) associated genes, and proteolytic enzymes were found to associate with ADe. Interestingly, ECM related genes were mainly detected in the up-regulated patterns, implied that they are closely associated with ADe pathogenesis in the local tissues. RT-PCR results consistently showed that FceRI, fatty acid binding protein 7 (FABP7) and carboxypeptidase A3 (CPA3) were up-regulated while arylsulfatase F (ARSF), amphiregulin (AREG), gap junction protein beta 2 (GJB2), glycolipid transfer protein (GLTP), hyaluronan synthase 3 (HAS3), and histone 4H4 (HIST4H4) detected as down-regulated in ADe patients. The large scale microarray provided information to figure out the AD pathogenesis and its complexity, especially for the extrinsic type of AD. In conclusion, several points have been proposed after conducting the present study: i) ECM and cell adhesion genes can be associated with AD disease and importantly contribute to AD pathogenesis; ii) the various functions of the classified up- or down-regulated genes implied the complexity of AD disease and difficulties for the clinical treating; iii) microarray technique adapting the RT-PCR method can enhance the validity of probing the critical AD-associated factors.

Key Words: Atopic dermatitis; Extrinsic type; Human oligochip microarray; Lesional skin biopsy; Primary cultured fibroblasts; SAM; RT-PCR

목차 (Table of Contents)

• 1.서론-----------------------8
• 2.대상 및 방법----------------11
• 3.결과 및 도해----------------14
• 4.고찰-----------------------48
• 5.참고문헌-------------------54