Characterization of Ha29, a Specific Gene for Helicoverpa armigeraSingle-nucleocapsid Nucleopolyhedrovirus

다국어 초록 (Multilingual Abstract)

Open reading frame 29 (ha29) is a gene specific for Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearSNPV). Sequence analyses showed that the transcription factor Tfb2 motif, bromodomain and Half-A-TPR (HAT) repeat were present at aa 66-82, 4-76, 55-90 of the Ha29 protein respectively. The product of Ha29 was detected in HearSNPV-infected HzAM1 cells at 3 h post-infection. Western blot analysis using a polyclonal antibody produced by immunizing a rabbit with purified GST-Ha29 fusion protein indicates that Ha29 is an early gene. The size of Ha29 product in infected HzAM1 cells was about 25 kDa, which was larger than the presumed size of 20.4 kDa. Tunicamycin treatment of HearSNPV-infected HzAM1 cells suggested that the Ha29 protein is N-glycosylated.

Fluorescent confocal laser scanning microscope examination, and Western blot analysis of purified budded virus (BVs), occlusion-derived virus (O DVs), cell nuclear and cytoplasmic fraction, showed that the Ha29 protein was localized in the nucleus. Our results suggested that ha29 of HearSNPV encodes a non-structurally functional protein that may be associated with virus gene transcription in Helicoverpa hosts.

참고문헌 (Reference)

1 Virus Res, "sequence analysis and phylogeny of the lef-2 gene of Helicoverpa armigera single nucleocapsid baculovirus" 65 : 1999

2 Pagni, "its status in 2002" 30 : 235-238, 2002

3 King, "in Insect Pests of Cotton" Heliothis/Helicoverpa armigera (ctuidae) : 445-467, 1994

4 and Keller, "a repetitive motif implicated in RNA processing" 23 : 15-16, 1998

5 Haynes, "a conserved sequence found in human" 2603-2603, 1992

6 null, "Virus Genes" 22 : 120-, 2001

7 San Diego, "Transcriptional Regulation in Eukaryotes" Academic Press Cold Spring Harbor Laboratory Press 127 1-155 50, 19902000

8 and Hu, "The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome" za (za): 241-257, 2001

9 Genet, "The role of Brahma and related proteins in transcription and development Characterization of Helicoverpa armigera nucleopolyhedrovirus orf33 that encodes a novel budded virion derived protein" in press 5 : 473-477, 1995

10 Fang, "The open reading frame 94 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel occlusion-derived virion protein" 84 : 2003

11 Lagrange, "The general transcriptional factors of RNA polymerase II" 10 : 2657-2683, 1996

12 Goldman, "Subcellular localization of genes and their products" Cold Spring Harbor Laboratory Press 1998

13 Blissard, "Seventh Report of the International Committee on Taxonomy of Viruses" 2000

14 Jarvis, "Requirements for nuclear localization and supramolecular assembly of a baculovirus polyhedrin protein" 795-185 810, 1991

15 Friesen, "Regulation of baculovirus early gene expression" Plenum Press 1997

16 Long, "Open reading frame 122 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel structural protein of occlusion-derived virions" 84 : 115-121, 2003

17 Chen, "Nucleotide sequence analysis of the polyhedrin gene of Heliothis armigera single nucleocapsid nuclear polyhedosis virus" 12 : 346-353, 1997

18 Schultz, "Identification of signaling domains" 95 : 1998

19 Nakai, "Genome sequence and organization of a nucleopolyhedrovirus isolated from the smaller tea tortrix" 36 : 171-316 783, 2003

20 Wood, "Genetically engineered baculoviruses as agents for pest control" 1991

21 Zhang, "Comparison of the complete genome sequences between C1 and G4 isolates of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus" 333 : 190-199, 2005190-199

22 Chen, "Comparative analysis of the genome sequences of Helicoverpa zea and Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus Structure and ligand of a histone acetyltransferase bromodomain" 83 : 491-399 496, 20021999

23 U, "Cleavage of structural proteins during the assembly of the head of the bacteriophage T4" -227, 1970

24 Wu, "Characterization of a unique OpMNPV-specific early gene not required for viral infection in tissue culture" 227 : 447-227 459, 1997

25 Wang, "Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus" 100 : 179-189, 2004

26 Goldbach, "A novel baculovirus envelope fusion protein with a proprotein convertase cleavage site" 275 : 30-275 41, 2000