A New Rapid Spectrophotometric Quantitative Determination Method for γ-Decalactone and Application in High-throughput Screening for γ-Decalactone Producing Strains

다국어 초록 (Multilingual Abstract)

A new, rapid spectrophotometric quantitativedetermination method for γ-decalactone (GDL) was developedbased on the ferric hydroxamate reaction. Samples addedto a mixture of NH2OH·HCl and NaOH were heated toreact. Then HCl was added. Ferric chloride was added forcoloration, and 75% (v/v) ethanol was added. Absorbancevalues were measured after coloring for 10 min. Theconcentration range of a GDL calibration curve was 0.125-8.14 g/L. A 96-well plate high-throughput screening methodwas established to obtain desirable strains. A total of 215GDL-producing strains were identified from 4,327 samples.
The highest production was 179 mg/L.

참고문헌 (Reference)

1 Cardillo R, "Stereochemistry of the microbial generation of .delta.-decanolide, .gamma.-dodecanolide, and .gamma.-nonanolide from C 18 13-hydroxy, and C 19 14-hydroxy unsaturated fatty acids" 56 : 5237-5239, 1991

2 Shakuntala, SS., "Spectrophotometric determination of traces of platinum with 5-chloro-2-hydroxythiobenzhydrazide after extraction into molten naphthalene" 26 : 95-100, 2010

3 Takeshi U, "Spectrophotometric determination of trace phosphate ions by amplitude-modulated flow analysis coupled with malachite green method" 26 : 797-801, 2010

4 Wang Y, "Spectrophotometric determination of cysteine with gold nanoparticles stabilized with single-stranded oligonucleotides" 26 : 545-549, 2010

5 Feron G, "Prospects for the microbial production of food flavours" 7 : 285-293, 1996

6 Buzzini P, "Production of volatile organic compounds (VOCs) by yeasts isolated from the ascocarps of black (Tuber melanosporum Vitt.) and white (Tuber magnatum Pico)truffles" 184 : 187-193, 2005

7 Gatfield IL., "Production of flavor and aroma compounds by biotechnology" 42 : 110-122, 1989

8 Farbood MI, "Production of Gamma-decalactone"

9 Arctander S., "Perfumes and Flavor Chemicals (Aroma Chemicals)" Montclair Press 23-38, 1969

10 Gervais P, "Osmotic accumulation of γ-decalactone by the yeast Sporidiobolus salmonicolor" 19 : 211-220, 1991

11 Wasserman BP, "Montville TJ, Korwek EL. Food Biotechnology" 42 : 133-146, 1988

12 Nelma G, "Isabel B. Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production by Yarrowia lipolytica" 90 : 1131-1137, 2013

13 Agudo M, "Impact of surfactants on the biotransformation of methyl ricinoleate into γ-decalactone by Yarrowia lipolytica" 29 : 31-36, 2004

14 Adelaide B, "Immobilization of Yarrowia lipolytica for aroma production from castor oil" 169 : 2202-2211, 2013

15 Khattab AA, "Genetic improvement of the β-oxidation pathway in the Yeast Yarrowia lipolytica to increase the production of γ-decalactone" 6 : 206-214, 2012

16 Nelma G, "Fed-batch versus batch cultures of Yarrowia lipolytica for γ-decalactone production from methyl ricinoleate" 34 : 649-654, 2012

17 Lin SJ., "Effect of various fatty acids components of castor oil on the growth and production of γ-decalactone by Sporobolomyces odorus" 82 : 42-45, 1996

18 Aguedo M, "Decalactone production by Yarrowia lipolytica under increased O2 transfer rates" 27 : 1617-1621, 2005

19 Zhong H, "Construction of high yield γ-decalactone strains by protoplast fusion technique" 230 : 102-105, 2011

20 Siek TJ, "Comparison of flavor thresholds of aliphatic lactones with those of fatty acids, esters, aldehydes, alcohols, and ketones" 54 : 1-4, 1971

21 Takashi S, "Collection of indonaphthol blue on a membrane filter for the spectrophotometric determination of ammonia with 1-naphthol and dichloroisocyanurate" 26 : 779-783, 2010

22 Xu Q, "Breeding on high yield γ-decalactone mutants by protoplast UV mutagenesis" 5 : 189-191, 2010

23 Mounier J, "Assessment of the microbial diversity at the surface of Livarot cheese using culture-dependent and independent approaches" 133 : 31-37, 2009

24 Schrader J, "Applied biocatalysis for the synthesis of natural flavour compounds--current industrial processes and future prospects" 26 : 463-472, 2004