TDU-GC/MS를 이용한 한국품종 참당귀 국산 판별 기법 = Geographical origin discrimination of Korean variety, Angelica gigas Nakai by using TDU (Thermal Desorption Unit)-GC/MS

다국어 초록 (Multilingual Abstract)

The objective of this study was to develop a fast and accurate method of variety discrimination and geographical discrimination origin of Korean angelica (Korean variety, Angelica gigas Nakai) by using TDU-GC/MS. Two peaks of decursin and decursinol, which are coumarin derivatives were identified in the range of Total Ion Chromatogram (TIC) RT 26.9-27.2 of the Korean angelica by GC/MS analysis at the time of condensation in a refrigerated condensation system after thermal desorption of sample extracts. In case of Chinese angelica (Chinese variety), ligustilide peak was detected at the RT 17.2. In order to investigate the difference of volatile components according to the geographical origin of Korean variety, the mass spectra were measured by TDU-GC/MS at the range of m/z 40-400 amu. The TIC of domestic cultivation and Chinese cultivation of the Korean variety, Angelica gigas Nakai showed the same tendency as a whole. However, in partial scans of TIC, two peaks detected at 15.54 and 16.05 of RT showed different peak patterns between Korean angelica (Korean variety) cultivated in Korea and in China. The ratio of Peak A (RT 15.54) and B (RT 16.05) was 0.0- 0.2 for domestic cultivation and 0.5-2.8 for Chinese cultivation, confirming the possibility of discriminating origin by comparing the TIC peak pattern of TDU-GC/MS.

참고문헌 (Reference)

1 Seo HY, "Volatile organic compounds of Angelica gigas Nakio, Korean medicinal herb" 21 : 265-273, 2002

2 Choi SH, "Volatile flavor components of Angelica gigas Nakai by the storage conditions" 32 : 513-518, 2000

3 Sekizai H, "Studies on the variation of ligustilide content for cultivating process of Angelica acutiloba var. acutiloba Kitagawa" 38 : 361-362, 1984

4 Lay HL, "Studies on the production and improvement in quality of Angelica acutiloba Kitagawa (1) Effects of manurial elements on the plant growth and yield, extract contents, ligustilide and butylidene phthalide contents of Angelica radix" 46 : 321-327, 1992

5 Ryu KS, "Studies on the coumarin constituents of the root of Angelica gigas Nakai. Isolation of decursionol angelate and assay of decursinol angelate and decursin" 21 : 64-68, 1990

6 Chi HJ, "Studies on essential oils of plants Angelica genus in Korea (1) Essential oils of Angelica gigantis radix" 19 : 239-247, 1988

7 Noro Y, "Pharmacognostical studies of Angelica radix (1) Phthalide content of wild angelica strains" 43 : 55-58, 1989

8 Choi HS, "Constituents of the essential oil of cnidium officinale Makino, a Korean medicinal plant" 17 : 49-53, 2002