Chlorinated aliphatic hydrocarbons (CAHs) represent a major class of pollutants due to their widespread distribution in environments and their toxic and/or carcinogenic properties. Among them, Chloroform (CE) and dichloromethane (DCM) are known to be ...
Chlorinated aliphatic hydrocarbons (CAHs) represent a major class of pollutants due to their widespread distribution in environments and their toxic and/or carcinogenic properties. Among them, Chloroform (CE) and dichloromethane (DCM) are known to be carcinogens in animals and suspected carcinogens in human. Especially CF is resistant to biodegradation in aquifer environments and persists for extended periods of times. In this paper, Methylosinus tichosporium OB3b was used for the treatments of CF and DCM via cometabolic biotransformations. In varying concentrations from 20μM to 80μM, V_max values were calculated as 5.88∼65nmol/(min·mg protein) and 37.1∼65.3nmol/(min·mg protein), respectively, for resting cells, 256∼947 nmol/(min·mg protein) for CF by non-washed cells.