The chemistry and engineering of mussel-inspired glue matrix for tissue adhesive and hemostatic

다국어 초록 (Multilingual Abstract)

The present study aimed to develop a bio-adhesive material with excellent hemostatic and rapid woundhealing properties using a catechol moiety (2chloro-30,40-dihydroxyacetophenone) conjugated to poly(dimethyl amino ethyl methacrylate-co-t-butyl methacrylate) [poly(DMA-co-t-BMA)-2-chloro-30,40-dihydroxyacetophenone (CA), C-PDB]. The desired glue matrix was formed by crosslinking between CPDBand chitosan via the Michael addition reaction. During glue matrix formation, chitosan plays a vitalrole as a cross-linker because the amine group of chitosan can easily react with the quinone from CA,based on catechol chemistry under mild alkaline conditions. Lap shear strength tests showed that theformulated glue matrix had a significant adhesive force at around 1.288 MPa and it formed quickly invitro. In addition, when applied on a bleeding site on a rat aorta in vivo, the prepared glue matrixdisplayed strong hemostatic behavior. Hematoxylin and eosin (H&E), Masson’s trichrome, and reticulinstaining of wounds (hepatic tissue) treated with the prepared glue matrix displayed rapid wound healing.
Therefore, the prepared glue matrix is a potential candidate as a hemostatic and wound-healing agent forclinical applications.

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