이황화탄소의 요중 대사물질에 관한 연구 = A Study on the Urinary Metabolites of Carbon Disulfide in Rats

다국어 초록 (Multilingual Abstract)

The objectives of this study are developing the optimal analytic methods for detecting urinary metabolites of carbon disulfide(CS₂) and determining which metabolite is more useful biological indicator for CS₂metabolites, For this experiment, we used synthesized CS₂metabolites, 2-thio-thiazolidine-4-carboxylic acid(TTCA) and thiocarbamide. Those were identified by infrared spectroscopy and nuclear magnetic resonance spectroscopy. The maximum absorbances of TTCA and thiocarbamide were 272 nm and 236 nm respectively. Detection by UV detector was more precise and accurate than that by infrared spectrophotometer. Ethyl acetate extraction method was adopted as precleaning method for HPLC analysis of urinary methabolites. Urinary amount of TTCA and thiocarbamide were measured by HPLC after administration of CS₂(350mg/kg, 700mg/kg) into Sprague-Dawley rats intraperitoneally. Excreted amounts of urinary TTCA and thiocarbamide were increased by the doses of CS₂administration. The urinary amount of TTCA was greater than that of thiocarbamide after administration of both doses of CS₂in rats. These results showed that TTCA seened to be more useful biological indicator for CS₂exposure as ACGIH recommended, however further studies are need to simplyfy analytic methods for measuring the urinary metabolites of CS₂and clarify mechanisms of CS₂metabolism.