코로나 바이러스 돼지유행성설사병 진단법의 비교 = Comparison between the Diagnostic Methods of the Porcine Epidemic Diarrhea Coronavirus

다국어 초록 (Multilingual Abstract)

Porcine epidemic diarrhea (PED) is acute viral enteric disease of swine of all ages by a coronavirus, PEDV. Recently, it has been reported that reliable diagnosis is accomplished successfully by vius isolation, RT-PCR, monoclonal antibody-based immunohistochemistry (IHC) and in situ Hybrydization (ISH). The major objective of this study was to compare the diagnostic methods for the detection of PEDV. The techniques were tested on the intestinal tissues and the formalin-fixed paraffin-embedded intestinal tissues from 28 piglets naturally infected with PEDV. As its results, 100% among tested animals were diagnosed by RT-PCR. PEDV antigens were demonstrated in 43% piglets by IHC. PEDV isolation were demonstrated in 4% by cell culture. Positive enterocytes were distributed over the tip and along the sides of atrophied or fused villi in the jejunum and ileum. However, PEDV RNAs were detected in 68% cases. Positive cells typically had dark brown reaction products in the cytoplasms of enterocytes. Therefore, ISH was more sensitive than IHC for the detection of PEDV. Furthermore, the reaction of ISH was more strong than that of IHC as a result of its testing. These results of this study indicate that RT-PCR and ISH could be used as a effective tool for the detection of PEDV.